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Simultaneous Determination of 42 Pesticides and Herbicides in Chicken Eggs by UHPLC–MS/MS and GC–MS Using a QuEChERS-Based Procedure

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Abstract

A quick, easy, cheap, rugged, effective, and safe (QuEChERS)-based method has been validated for the extraction of 42 pesticides and herbicides including organophosphorus pesticides (OPPs), carbamate pesticides (CBs), herbicides (HBs), organochlorine pesticides (OCPs), and synthetic pyrethroid pesticides (PYRs) from chicken eggs. The QuEChERS-based extraction procedure was followed by cleanup steps using C18 and primary secondary amine sorbents. The supernatant was analyzed by ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) and gas chromatography–mass spectrometry (GC–MS). The OPPs, CBs, and HBs were quantified by UHPLC–MS/MS, while the OCPs and PYRs were detected by GC–MS. The limits of quantification ranged from 0.01 to 8.5 μg kg−1, and the analyte recoveries were in the range of 64.9–123.2 %. Furthermore, the repeatabilities (intra-day and inter-day) were good, and linear matrix-matched calibration curves were obtained. Acetochlor was identified in concentrations ranging from 0.27 to 0.44 μg kg−1 in four samples from 80 chicken eggs. The method was successfully demonstrated for the fast and reliable analysis of pesticides and herbicides in chicken egg samples.

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Acknowledgments

This research was supported by the Special Foundation for Young Scientists of Hubei Provincial Centre for Disease Control and Prevention (Y2013W01, Y2013W07) and the Public Sector Program of the National Environmental Protection of the People’s Republic of China (No. 201309046) and the Project of Hubei Collaborative Innovation Center for Security Precaution and Emergency Response Technology (JD2016010201). Meanwhile, we thank Elixigen Corporation (Huntington Beach, California, USA) for helping in proofreading and editing the English of final manuscript.

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Authors and Affiliations

  1. National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430079, China

    Yonggang Li & Meihu Ma

  2. Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430079, China

    Yonggang Li, Ziliang Chen, Rui Zhang, Ping Luo, Yan Zhou & Sheng Wen

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  1. Yonggang Li
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  2. Ziliang Chen
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  3. Rui Zhang
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  5. Yan Zhou
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  7. Meihu Ma
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Correspondence to Sheng Wen or Meihu Ma.

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Li, Y., Chen, Z., Zhang, R. et al. Simultaneous Determination of 42 Pesticides and Herbicides in Chicken Eggs by UHPLC–MS/MS and GC–MS Using a QuEChERS-Based Procedure. Chromatographia 79, 1165–1175 (2016). https://doi.org/10.1007/s10337-016-3132-y

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  • DOI: https://doi.org/10.1007/s10337-016-3132-y

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