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Determination of Free Fatty Acids of Chinese Coriandrum sativum L. Using Benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate as Precolumn Labeling Reagent by LC with Fluorescence Detection

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Abstract

A new fluorescent labeling reagent, benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate (BQETS) was designed and synthesized, and it was successfully applied to the determination of fatty acids with liquid chromatography. BQETS can easily and quickly label fatty acids within 20 min at 90 °C in dimethylformamide with K2CO3 as catalyst. The derivatives exhibit high stability and strong fluorescence with excitation and emission wavelengths of 247 and 401 nm, respectively. The 24 derivatives of fatty acids were completely separated by gradient elution on a Hypersil GOLD C18 column. Excellent linear responses for all fatty acids were observed with correlation coefficients of >0.9991. The method also showed good sensitivity and precision, with limits of detection in the 0.0024–0.0206 μg g−1 range and relative standard deviations ≤9.6 %. This is the first time that BQETS fluorescent probe and its applications for the determination of fatty acids have been reported. Moreover, this is the first report on the comparison of free fatty acids composition in the above-ground part of Coriandrum sativum L. from different habitats in China.

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Acknowledgments

The research was supported by the National Natural Science Foundation of China (Nos. 21275089, 21475075).

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Authors and Affiliations

  1. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People’s Republic of China

    Yanan Sun, Zhongyin Ji & Jinmao You

  2. Shandong Province Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu, People’s Republic of China

    Xia Zhang, Cuihua Song, Zhiwei Sun & Jinmao You

  3. Graduate University of the Chinese Academy of Science, Beijing, People’s Republic of China

    Yanan Sun & Zhongyin Ji

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  1. Yanan Sun
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  2. Xia Zhang
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  3. Zhongyin Ji
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  5. Zhiwei Sun
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Correspondence to Zhiwei Sun.

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Sun, Y., Zhang, X., Ji, Z. et al. Determination of Free Fatty Acids of Chinese Coriandrum sativum L. Using Benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate as Precolumn Labeling Reagent by LC with Fluorescence Detection. Chromatographia 79, 547–559 (2016). https://doi.org/10.1007/s10337-016-3071-7

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