Abstract
To analyze genetic relationships among Colletotrichum gloeosporioides isolates; 34 isolates were collected from strawberries all over Japan, but primarily from Chiba Prefecture, and 20 were isolated from hosts other than strawberry. These isolates were assayed for virulence on strawberries and subjected to a fingerprint (FP) analysis by repetitive extragenic palindromic-PCR. Most of the isolates that were highly virulent to strawberries had relatively similar FPs even though they were isolated from various host plants (strawberry, cyclamen, nipplefruit and Japanese pear) in different regions. A cluster analysis revealed that the highly virulent and the weakly virulent isolates tended to form individual clusters, indicating that the highly virulent strains of C. gloeosporioides, which were genetically close to isolates with similar FPs and distinguishable from the weakly virulent strains, were responsible for strawberry anthracnose in Japan.
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Acknowledgments
This study was part of a project entitled “Development of Quick Diagnostic Methods for Latent Infection by Strawberry Anthracnose and Drug Resistant Pathogens”, funded by the research and development projects for application in promoting new policy of Agriculture Forestry and Fisheries, 2009. Dr. Yoshiaki Chikuo of National Institute of Floricultural Science, National Agriculture and Food Research Organization, Independent Administrative Corporation; Dr. Seiju Ishikawa of Tochigi Prefectural Agricultural Experiment Station; Mr. Mitsutaka Mori of Kagawa Prefectural Experiment Station; Mr. Hiroshi Sakai of Gunma Agricultural Technology Center; Mr. Yoshihiko Hirayama of Nara Prefectural Agricultural Experiment Station and Mr. Masahiro Nao of Ehime Research Institute of Agriculture, Forestry and Fisheries kindly provided us with fungal isolates for this study. We thank them very much.
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Suzuki, T., Tanaka-Miwa, C., Ebihara, Y. et al. Genetic polymorphism and virulence of Colletotrichum gloeosporioides isolated from strawberry (Fragaria × ananassa Duchesne). J Gen Plant Pathol 76, 247–253 (2010). https://doi.org/10.1007/s10327-010-0242-5
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DOI: https://doi.org/10.1007/s10327-010-0242-5