Abstract
There is a high demand for immobilized enzymes in the industry because it is a green technology, it improves the efficiency of the crude enzyme and it minimizes the step of product recovery. Selection of a suitable carrier for enzyme immobilization is crucial. Eggshells are abundantly available as an organic waste. Here, lipase from Thermomyces lanuginosus was immobilized on eggshell to produce butyl butyrate. We evaluated the effect of lipase loading, from 0.5 to 3.0 %, size of eggshells, lower than 1, 1–2 and 2–4 mm, and temperature, from 30 to 50 °C. Results show that the optimum conditions calculated by response surface methodology analysis for butyl butyrate synthesis were 40 % w/v of immobilized lipase loading and 0.2 M of substrate concentration at 38 °C during 24 h. The predicted yield was 92.78 %, and the yield obtained during synthesis was 93.48 %.
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References
Berger RG, Krings U, Zorn H (2010) Biotechnological flavour generation. In: Taylor AJ, Linforth RST (eds) Food flavour technology. Wiley, Oxford, pp 89–126
Bosley JA, Peilow AD (1997) Immobilization of lipases on porous polypropylene: reduction in esterification efficiency at low loading. J Am Oil Chem Soc 74(2):107–111
Bueno PRM, Ferreira De Oliveira T, Caliari M, Castiglioni GL, Soares Junior MS (2014) Selection and optimization of extracellular lipase production using agro-industrial waste. Afr J Biotechnol 13(4):566–573
Chang SF, Chang SW, Yen YH, Shieh CJ (2007) Optimum immobilization of Candida rugosa lipase on Celite by RSM. Appl Clay Sci 37(1–2):67–73
Fernandes MLM, Krieger N, Baron AM, Zamora PP, Ramos LP, Mitchell DA (2004) Hydrolysis and synthesis reactions catalysed by Thermomyces lanuginosa lipase in the AOT/Isooctane reversed micellar system. J Mol Catal B Enzym 30(1):43–49
Js M et al (2014) Immobilization of Thermomyces lanuginosus lipase on mesoporous poly-hydroxybutyrate particles and application in alkyl esters synthesis: isotherm, thermodynamic and mass transfer studies. Chem Eng J 251:392–403
Lorenzoni ASG, Graebin NG, Martins AB, Fernandez-Lafuente R, ZáchiaAyub MA, Rodrigues RC (2012) Optimization of pineapple flavour synthesis by esterification catalysed by immobilized lipase from Rhizomucor miehei. Flavour Fragr J 27(2):196–200
Makkar HP, Sharma OP (1983) Egg shell as a carrier for enzyme immobilization. Biotechnol Bioeng 25(2):595–597
Martins AB, Friedrich JLR, Cavalheiro JC, Garcia-Galan C, Barbosa O, Ayub MAZ, Fernandez-Lafuente R, Rodrigues RC (2013a) Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene–divinylbenzene beads. Bioresour Technol 134:417–422
Martins AB, Friedrich JLR, Rodrigues RC, Garcia-Galan C, Fernandez-Lafuente R, Ayub MAZ (2013b) Optimized butyl butyrate synthesis catalyzed by Thermomyces lanuginosus lipase. Biotechnol Prog 29(6):1416–1421
Monot F, Borzeix F, Bardin M, Vandecasteele J-P (1991) Enzymatic esterification in organic media: role of water and organic solvent in kinetics and yield of butyl butyrate synthesis. Appl Microbiol Biotechnol 35(6):759–765
Peterson ME, Eisenthal R, Danson MJ, Spence A, Daniel RM (2004) A new intrinsic thermal parameter for enzymes reveals true temperature optima. J Biol Chem 279(20):20717–20722
Pinang U. perancang dan penilaian J.P.V.P., 2015. Perangkaan_Ternakan.pdf., p.11. http://www.jpvpp.gov.my/index.php/my/peraturan/statistik-jabatan. Accessed 23 Jan 2016
Santos JC, De Castro HF (2006) Optimization of lipase-catalysed synthesis of butyl butyrate using a factorial design. World J Microbiol Biotechnol 22:1007–1011
Stadelman WJ (2000) Eggs and egg products. In: Francis FJ (ed) Encyclopedia of food science and technology, 2nd ed. Wiley, New York, pp 593–599
Tsai, WT et al (2006) Characterization and adsorption properties of eggshells and eggshell membrane. Bioresour Technol 97(3):488–493
Tsai WT, Hsien KJ, Hsu HC, Lin CM, Lin KY, Chiu CH (2008) Utilization of ground eggshell waste as an adsorbent for the removal of dyes from aqueous solution. Bioresour Technol 99(6):1623–1629
Vemuri G, Banerjee R, Bhattacharyya BC (1998) Immobilization of lipase using egg shell and alginate as carriers: optimization of reaction conditions. Bioprocess Eng 19(2):111–114
Xavier Malcata F et al (1990) Immobilized lipase reactors for modification of fats and oils—a review. J Am Oil Chem Soc 67(12):890–910
Yücel Y (2012) Optimization of immobilization conditions of Thermomyces lanuginosus lipase on olive pomace powder using response surface methodology. Biocatal Agric Biotechnol 1(1):39–44
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This work was supported by Kementerian Pendidikan Malaysia (KPM) Fundamental Research Grant Scheme FRGS (6711374), Transdisciplinary Research Grant Scheme TRGS (6762003) and KPM MyMaster.
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Salleh, S., See, Y.S., Serri, N.A. et al. Synthesis of butyl butyrate in 93 % yield by Thermomyces lanuginosus lipase on waste eggshells. Environ Chem Lett 14, 189–194 (2016). https://doi.org/10.1007/s10311-016-0553-7
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DOI: https://doi.org/10.1007/s10311-016-0553-7