Abstract
An in vitro clonal propagation procedure for mature Tectona grandis (teak) trees is described. Multiple shoots were induced from nodal segments through axillary bud proliferation. A shoot multiplication rate of 6.33 was achieved on Murashige and Skoog's (MS) medium supplemented with 10 µM 6-benzyladenine BA and 1 µM 1-naphthalene acetic acid (NAA) during every subculture cycle of 4 weeks. In vitro raised shoots could be successfully rooted (66.66%) on liquid MS medium supplemented with 15 µM NAA, with 1.60 roots per shoot, every 6 weeks of culture. In vitro hardening was carried out in sand soaked with half-strength MS medium (organic free). The plantlets were acclimatized first in a mist chamber and then in polybags in a mixture of soil, sand, and farmyard manure (1 : 1 : 1 v/v) in a shade house.
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Shirin, F., Rana, P. & Mandal, A. In vitro clonal propagation of mature Tectona grandis through axillary bud proliferation. J For Res 10, 465–469 (2005). https://doi.org/10.1007/s10310-005-0173-8
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DOI: https://doi.org/10.1007/s10310-005-0173-8