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Free radical scavenging and cytotoxic activity of seed extracts of Podophyllum hexandrum

Piégeage des radicaux libres et activité cytotoxique des graines extraites du Podophyllum hexandrum

  • Pharmacognosie
  • Published:
Phytothérapie

Abstract

In the present study, in vitro antioxidant and cytotoxic activities of different extracts of Podophyllum hexandrum seed were investigated. Different polarities crude extracts were prepared through Soxhlet extractor and were used for the determination of antioxidant potential by 2,2′-diphenyl-1-picrylhydrazy (DPPH), reducing power, microsomal lipid peroxidation, and hydroxyl radical scavenging activity. Cytotoxic activity was carried out by the Sulphorhodamine B (SRB) method. The highest phenolic content of 465 ± 5.55 mgGallic Acid Equivalent(GAE) /g was observed in methanol extract followed by ethanol (359 ± 8.52) and aqueous extract (291 ±4.5 mgGAE/g). At a concentration of 700 μg/ml, DPPH radical scavenging activity was found to be highest in methanol extract (86 ±1.5%) followed by ethanol extract (78 ± 1.2), aqueous extract (71 ± 1.5), and ethyl acetate extract (64 ± 1.5%). At a concentration of 300 μg/ml, 76%, 65%, 65%, and 60% inhibition on microsomal lipid peroxidation was observed with methanol, ethanol, aqueous, and ethyl acetate extracts, respectively. Cytotoxicity of the extracts (100 μg/ml) was tested on three human cancer cell lines (MCF-7, HCT-116, and HeLa) using the SRB assay. These results clearly indicate that Podophyllum hexandrum seed extracts possess free radical savaging and cytotoxic activity and can be used against various oxidative stressrelated pathological conditions.

Résumé

Dans la présente étude, les activités cytotoxique et antioxydante in vitro de différents extraits de Podophyllum hexandrum graines ont été étudiées. Différentes polarités des extraits bruts ont été préparées grâce à l’extracteur Soxhlet et ont été utilisées pour la détermination du potentiel antioxydant par 2,2′-diphenyl-1-picrylhydrazy (DPPH), réduisant ainsi la puissance, la peroxydation des lipides microsomiques et activité piégeur de radicaux hydroxyles. L’activité cytotoxique a été réalisée par méthode de SRB. La plus haute teneur en composés phénoliques (PTC) de 465 ± 5,55 mgÉquivalent Acide Gallique (EAG)/g a été observée dans le méthanol extrait suivi par l’éthanol (359 ± 8,52) et extrait aqueux (291 ± 4,5 mgEAG/g). À la concentration de 700 μg/ml, DPPH antiradicalaires plus élevés de l’activité extrait au méthanol (86 ± 1,5 %), suivi par extraction à l’éthanol (78 ± 1,2), de l’extrait aqueux (71 ± 1,5) et extrait de l’acétate d’éthyle (64 ± 1,5 %). À la concentration de 300 μg/ml, 76, 65, 65 et 60 % d’inhibition de la peroxydation des lipides microsomiques ont été observées avec le méthanol, l’éthanol et l’acétate d’éthyle aqueux extraits respectivement. La cytotoxicité des extraits (100 μg/ml) a été testée sur trois lignées cellulaires cancéreuses humaines (MCF-7, HCT-116 et HeLa) à l’aide du sulphorhodamine B assay. Ces résultats indiquent clairement que les espèces Podophyllum hexandrum extraits de semence possèdent des radicaux libres éreintants et une activité cytotoxique et peuvent être employées contre diverses conditions pathologiques liées au stress oxydatif.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, University of Kashmir, Hazratbal, Srinagar, 190006, India

    O. Zargar, K. Bashir Dar, A. H. Bhat, M. Afzal Zargar, A. Masood & A. G. Showkat

  2. Department of Biochemistry, University of Kashmir, Hazratbal, Srinagar, 190006, India

    R. Hamid & A. G. Showkat

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  1. O. Zargar
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  2. R. Hamid
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  3. K. Bashir Dar
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  5. M. Afzal Zargar
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  7. A. G. Showkat
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Correspondence to A. G. Showkat.

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Zargar, O., Hamid, R., Bashir Dar, K. et al. Free radical scavenging and cytotoxic activity of seed extracts of Podophyllum hexandrum . Phytothérapie (2016). https://doi.org/10.1007/s10298-016-1072-1

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  • DOI: https://doi.org/10.1007/s10298-016-1072-1

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