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A new approach for improving epothilone B yield in Sorangium cellulosum by the introduction of vgb epoF genes

  • Biotechnology Methods
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Epothilone B has drawn great attention due to its much stronger anticancer activity and weaker side effects compared with taxol. The relative low yield of epothilone B limited its application. In this study, we report the successful introduction of the vgb gene and the epoF gene into Sorangium cellulosum So ce M4 by electroporation for the first time, which was demonstrated by Southern blot analysis. Results of qRT-PCR, SDS-PAGE and western blot analysis confirmed the transcription and expression of the vgb and epoF genes. LC–MS results showed that the epothilones B, A yields were improved and epothilones D, C yields were decreased. The yields of epothilone B were improved by 57.9 ± 0.3, 62.7 ± 0.8 and 122.4 ± 0.7 % through the introduction of vgb gene, epoF gene and both genes into strain So ce M4, respectively. Our study provides a new approach for improving epothilone B yield in S. cellulosum.

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Abbreviations

VHb:

Vitreoscilla hemoglobin

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Acknowledgments

We gratefully acknowledge financial support from National Natural Science Foundation of China (31500037), Natural Science Foundation of Guangdong Province (2015A030310103), the Science Foundation for the Youth Scholars of Guangdong Academy of Science (No. qnjj201402).

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Authors and Affiliations

  1. State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, 510070, China

    Wei Ye, Weimin Zhang, Yuchan Chen, Haohua Li, Saini Li, Qingling Pan, Guohui Tan & Taomei Liu

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  1. Wei Ye
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  2. Weimin Zhang
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  3. Yuchan Chen
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Correspondence to Weimin Zhang.

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10295_2016_1735_MOESM1_ESM.tif

Fig. S1 The detection of epothilones in different So ce M4 strains by LC–MS/MS: ion peaks at 492.8, and 508.8 were corresponding to epothilones B and D, respectively, and average abundance of each epothilone ion peak was also listed (TIFF 1979 kb)

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Ye, W., Zhang, W., Chen, Y. et al. A new approach for improving epothilone B yield in Sorangium cellulosum by the introduction of vgb epoF genes. J Ind Microbiol Biotechnol 43, 641–650 (2016). https://doi.org/10.1007/s10295-016-1735-9

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  • DOI: https://doi.org/10.1007/s10295-016-1735-9

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