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Butanol production by Clostridium beijerinckii ATCC 55025 from wheat bran

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Wheat bran, a by-product of the wheat milling industry, consists mainly of hemicellulose, starch and protein. In this study, the hydrolysate of wheat bran pretreated with dilute sulfuric acid was used as a substrate to produce ABE (acetone, butanol and ethanol) using Clostridium beijerinckii ATCC 55025. The wheat bran hydrolysate contained 53.1 g/l total reducing sugars, including 21.3 g/l of glucose, 17.4 g/l of xylose and 10.6 g/l of arabinose. C. beijerinckii ATCC 55025 can utilize hexose and pentose simultaneously in the hydrolysate to produce ABE. After 72 h of fermentation, the total ABE in the system was 11.8 g/l, of which acetone, butanol and ethanol were 2.2, 8.8 and 0.8 g/l, respectively. The fermentation resulted in an ABE yield of 0.32 and productivity of 0.16 g l−1 h−1. This study suggests that wheat bran can be a potential renewable resource for ABE fermentation.

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References

  1. Atsumi S, Higashide W, Liao JC (2009) Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. Nat Biotechnol 27:1177–1180

    Article  CAS  PubMed  Google Scholar 

  2. Atsumi S, Hanai T, Liao JC (2008) Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature 451:86–89

    Article  CAS  PubMed  Google Scholar 

  3. Berezina OV, Brandt A, Yarotsky S, Schwarz WH, Zverlov VV (2009) Isolation of a new butanol-producing Clostridium strain: high level of hemicellulosic activity and structure of solventogenesis genes of a new Clostridium saccharobutylicum isolate. Syst Appl Microbiol 32:449–459

    Article  CAS  PubMed  Google Scholar 

  4. Bergmans MEF, Beldman G, Gruppen H, Voragen AGJ (1996) Optimisation of the selective extraction of (glucurono) arabinoxylans from wheat bran: use of barium and calcium hydroxide solution at elevated temperatures. J Cereal Sci 23:235–245

    Article  CAS  Google Scholar 

  5. Bergmeyer HU, Grassel M (1983) Reagents for enzymatic analysis: enzymes-α-amylase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 2, 3rd edn. Verlag Chemie, Germany, pp 151–152

    Google Scholar 

  6. Bramucci MG, Nagarajan V, Sedkova N, Singh M (2009) Solvent tolerant microorganisms and methods of isolation. US Patent 7,541,173

  7. Choteborska P, Palmarola-Adrados B, Galbe M, Zacchi G, Melzoch K, Rychtera M (2004) Processing of wheat bran to sugar solution. J Food Eng 61:561–565

    Article  Google Scholar 

  8. Demain AL (2009) Biosolutions to the energy problem. J Ind Microbiol Biotechnol 36:319–332

    Article  CAS  PubMed  Google Scholar 

  9. Durre P (1998) New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation. Appl Microbiol Biotechnol 49:639–648

    Article  CAS  Google Scholar 

  10. Ezeji T, Blaschek HP (2008) Fermentation of dried distillers’ grains and solubles (DDGS) hydrolysates to solvents and value-added products by solventogenic clostridia. Bioresour Technol 99:5232–5242

    Article  CAS  PubMed  Google Scholar 

  11. Ezeji T, Qureshi N, Blaschek HP (2007) Butanol production from agricultural residues: impact of degradation products on Clostridium beijerinckii growth and butanol fermentation. Biotechnol Bioeng 97:1460–1469

    Article  CAS  PubMed  Google Scholar 

  12. Ezeji TC, Qureshi N, Blaschek HP (2007) Bioproduction of butanol from biomass: from genes to bioreactors. Curr Opin Biotechnol 18:220–227

    Article  CAS  PubMed  Google Scholar 

  13. Ezeji TC, Qureshi N, Blaschek HP (2004) Butanol fermentation research: upstream and downstream manipulations. Chem Rec 4:305–314

    Article  CAS  PubMed  Google Scholar 

  14. Green EM, Bennett GN (1998) Genetic manipulation of acid and solvent formation in Clostridium acetobutylicum ATCC 824. Biotechnol Bioeng 58:215–221

    Article  CAS  PubMed  Google Scholar 

  15. Jain MK, Beacom D, Datta R (1993) Mutant strain of C. acetobutylicum and process for making butanol. US Patent 5,192,673

  16. Jesse TW, Ezeji TC, Qureshi N, Blaschek HP (2002) Production of butanol from starch-based waste packing peanuts and agricultural waste. J Ind Microbiol Biotechnol 29:117–123

    Article  CAS  PubMed  Google Scholar 

  17. Johnson JL, Toth J, Santiwatanakul S, Chen JS (1997) Cultures of “Clostridium acetobutylicum” from various collections comprise Clostridium acetobutylicum, Clostridium beijerinckii, and two other distinct types based on DNA-DNA reassociation. Int J Syst Bacteriol 47:420–424

    Article  CAS  PubMed  Google Scholar 

  18. Jones DT, Woods DR (1986) Acetone-butanol fermentation revisited. Microbiol Rev 50:484–524

    CAS  PubMed  Google Scholar 

  19. Keasling JD, Chou H (2008) Metabolic engineering delivers next-generation biofuels. Nat Biotechnol 26:298–299

    Article  CAS  PubMed  Google Scholar 

  20. Kim Y, Mosier NS, Hendrickson R, Ezeji T, Blaschek H, Dien B, Cotta M, Dale B, Ladisch MR (2008) Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage. Bioresour Technol 99:5165–5176

    Article  CAS  PubMed  Google Scholar 

  21. Larsson S, Palmqvist E, Hahn-Hagerdal B, Tengborg C, Stenberg K, Zacchi G, Nilvebrant NO (1999) The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme Microb Technol 24:151–159

    Article  CAS  Google Scholar 

  22. Lee J, Seo E, Kweon DH, Park K, Jin YS (2009) Fermentation of rice bran and defatted rice bran for butanol production using Clostridium beijerinckii NCIMB 8052. J Microbiol Biotechnol 19:482–490

    Article  CAS  PubMed  Google Scholar 

  23. Ljungdahl LG (2009) A life with acetogens, thermophiles, and cellulolytic anaerobes. Annu Rev Microbiol 63:1–25

    Article  CAS  PubMed  Google Scholar 

  24. Maes C, Delcour JA (2001) Alkaline hydrogen peroxide extraction of wheat bran non-starch polysaccharides. J Cereal Sci 34:29–35

    Article  CAS  Google Scholar 

  25. Martinez A, Rodriguez ME, Wells ML, York SW, Preston JF, Ingram LO (2001) Detoxification of dilute acid hydrolysates of lignocellulose with lime. Biotechnol Prog 17:287–293

    Article  CAS  PubMed  Google Scholar 

  26. Ounine K, Petitdemange H, Raval G, Gay R (1985) Regulation and butanol inhibition of d-xylose and d-glucose uptake in Clostridium acetobutylicum. Appl Environ Microbiol 49:874–878

    CAS  PubMed  Google Scholar 

  27. Papoutsakis ET (2008) Engineering solventogenic clostridia. Curr Opin Biotechnol 19:420–429

    Article  CAS  PubMed  Google Scholar 

  28. Parekh SR, Wayman M (1988) Ethanol and butanol production by fermentation of enzymatically saccharified SO2-prehydrolysed lignocellulosics. Enzyme Microb Technol 10:660–668

    Article  CAS  Google Scholar 

  29. Qureshi N, Blaschek HP (1999) Butanol recovery from model solution/fermentation broth by pervaporation: evaluation of membrane performance. Biomass Bioenerg 17:175–184

    Article  CAS  Google Scholar 

  30. Qureshi N, Blaschek HP (2000) Economics of butanol fermentation using hyper-butanol producing Clostridium beijerinckii BA101. Food Bioprod Process 78:139–144

    Article  Google Scholar 

  31. Qureshi N, Saha BC, Cotta MA (2008) Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii. Part II—Fed-batch fermentation. Biomass Bioenerg 32:176–183

    Article  CAS  Google Scholar 

  32. Qureshi N, Saha BC, Hector RE, Cotta MA (2008) Removal of fermentation inhibitors from alkaline peroxide pretreated and enzymatically hydrolyzed wheat straw: production of butanol from hydrolysate using Clostridium beijerinckii in batch reactors. Biomass Bioenerg 32:1353–1358

    Article  CAS  Google Scholar 

  33. Qureshi N, Ezeji TC, Ebener J, Dien BS, Cotta MA, Blaschek HP (2008) Butanol production by Clostridium beijerinckii. Part I: use of acid and enzyme hydrolyzed corn fiber. Bioresour Technol 99:5915–5922

    Article  CAS  PubMed  Google Scholar 

  34. Qureshi N, Li XL, Hughes S, Saha BC, Cotta MA (2006) Butanol production from corn fiber xylan using Clostridium acetobutylicum. Biotechnol Prog 22:673–680

    Article  CAS  PubMed  Google Scholar 

  35. Qureshi N, Lolas A, Biaschek HP (2001) Soy molasses as fermentation substrate for production of butanol using Clostridium beijerinckii BA101. J Ind Microbiol Biotechnol 26:290–295

    Article  CAS  PubMed  Google Scholar 

  36. Qureshi N, Saha BC, Cotta MA (2007) Butanol production from wheat straw hydrolysate using Clostridium beijerinckii. Bioprocess Biosyst Eng 30:419–427

    Article  CAS  PubMed  Google Scholar 

  37. Ralet MC, Thibault JF, Della Valle G (1990) Influence of extrusion cooking on the physicochemical properties of wheat bran. J Cereal Sci 11:249–259

    Article  CAS  Google Scholar 

  38. Steen EJ, Chan R, Prasad N, Myers S, Petzold CJ, Redding A et al (2008) Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol. Microb Cell Fact 7:36

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by the Key Technologies R & D Program of Shandong Province (2007GG2QT07006), National Science Foundation of China (30970049), Research Project of Shandong Province Soft Science Program (2009RKB161), and State Key Laboratory of Microbial Technology of Shandong University.

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Authors and Affiliations

  1. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People’s Republic of China

    Ziyong Liu, Yu Ying & Fuli Li

  2. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People’s Republic of China

    Cuiqing Ma & Ping Xu

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Yu Ying

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  1. Ziyong Liu
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  2. Yu Ying
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  3. Fuli Li
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Correspondence to Fuli Li.

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Liu, Z., Ying, Y., Li, F. et al. Butanol production by Clostridium beijerinckii ATCC 55025 from wheat bran. J Ind Microbiol Biotechnol 37, 495–501 (2010). https://doi.org/10.1007/s10295-010-0695-8

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  • DOI: https://doi.org/10.1007/s10295-010-0695-8

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