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Taxonomic revision of Lophiostoma and Lophiotrema based on reevaluation of morphological characters and molecular analyses

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Mycoscience

Abstract

Lophiostoma and Lophiotrema share several morphological and ecological features. They have been regarded as closely related genera within the family Lophiostomataceae, but their morphological circumscriptions have been uncertain. To clarify the generic definitions of Lophiostoma and Lophiotrema, we conducted phylogenetic analyses of 29 isolates of these genera based on the SSU and LSU nrDNA sequences, and also reevaluated several key characters previously used for their generic characterization. Our results clearly confirmed that Lophiostoma and Lophiotrema are distinct genera belonging to different families; the ascus shape, including length of the ascus stipe, is a reliable taxonomic indicator to allow discrimination between the genera. In Lophiostoma species, asci are clavate with relatively long stipes [mostly (10–) 15–30 μm in length], whereas in Lophiotrema the asci are cylindrical with short stipes (up to 15 μm long). A new family, Lophiotremataceae, is proposed to accommodate species in the Lophiotrema clade that was distantly placed from the Lophiostomataceae within the Pleosporales. Lophiostoma quadrisporum, collected from twigs of Liriodendron tulipifera, is described as a new species with distinctive 4-spored asci. Lophiotrema vitigenum, which has clavate asci with long stipes, is transferred to Lophiostoma.

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Acknowledgments

This work was partially supported by grants from the Japan Society for the Promotion of Science (JSPS, 22770074), the Sasakawa Scientific Research Grant from the Japan Science Society, and the Hirosaki University Grant for Exploratory Research by Young Scientists (2008–2010). We thank Dr. Vadim A. Mel’nik (Komarov Botanical Institute, Russia) for reviewing the draft manuscript and our reviewers for their valuable comments.

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  1. Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori, 036-8561, Japan

    Kazuyuki Hirayama & Kazuaki Tanaka

  2. The United Graduate School of Agricultural Sciences, Iwate University, 18-8 Ueda 3 chome, Morioka, 020-8550, Japan

    Kazuyuki Hirayama

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  1. Kazuyuki Hirayama
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  2. Kazuaki Tanaka
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Correspondence to Kazuaki Tanaka.

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Hirayama, K., Tanaka, K. Taxonomic revision of Lophiostoma and Lophiotrema based on reevaluation of morphological characters and molecular analyses. Mycoscience 52, 401–412 (2011). https://doi.org/10.1007/s10267-011-0126-3

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  • DOI: https://doi.org/10.1007/s10267-011-0126-3

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