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Influence of porcine-derived collagen matrix on endothelial progenitor cells: an in vitro study

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Abstract

Porcine-derived collagen matrix (PDCM) has been reported as a promising alternative to autogenous soft tissue grafts in periodontal plastic surgery. The aim of this study was to analyze the influence of a novel PDCM on endothelial progenitor cells (EPC) in vitro. EPC were isolated from human peripheral blood, cultured and transferred on the PDCM (mucoderm®). Tissue culture polystyrene surface (TCPS) served as control. Cell viability of EPC on PDCM was measured by a MTT and PrestoBlue® assay. Migration ability was tested using a Boyden migration assay. A ToxiLight® assay was performed to analyze the influence of PDCM on adenylate kinase (ADK) release and apoptosis rate of EPC. Using the MTT assay, EPC cultured on PDCM demonstrated a significantly increased cell viability compared to the control group at days 3, 6 and 12 (p each <0.001). According to the PrestoBlue® assay, EPC showed a significant increase of cell viability compared to the control group at 48, 72, and 96 h (p each <0.001). In the Boyden migration assay, a significantly increased EPC migration ability could be observed after 3–12 days (p each ≤0.001). No significantly increased apoptosis rate of EPC on PDCM could be observed with exception after 96 h (p each >0.05). Overall, our results suggest a good biocompatibility of PDCM without any cytotoxic effects on EPC, which might support a rapid revascularization and therefore a sufficient ingrowth of the PDCM.

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Conflict of interest

The authors declare that there is no conflict of interests.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany

    Andreas Max Pabst, Christian Walter & Maximilian Krüger

  2. Department of Operative Dentistry and Periodontology, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany

    Adrian Kasaj

  3. Department of Prosthetic Dentistry, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany

    Karl-Martin Lehmann

  4. Department of Periodontology, Victor Babes University of Medicine and Pharmacology, Piata E. Murgu 2, 300041, Timisoara, Romania

    Stefan-Ioan Stratul

Authors
  1. Andreas Max Pabst
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  2. Karl-Martin Lehmann
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  3. Christian Walter
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  4. Maximilian Krüger
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  5. Stefan-Ioan Stratul
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  6. Adrian Kasaj
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Correspondence to Adrian Kasaj.

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Pabst, A.M., Lehmann, KM., Walter, C. et al. Influence of porcine-derived collagen matrix on endothelial progenitor cells: an in vitro study. Odontology 104, 19–26 (2016). https://doi.org/10.1007/s10266-014-0186-x

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  • DOI: https://doi.org/10.1007/s10266-014-0186-x

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