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Regulation of RNA metabolism is important for in vitro dedifferentiation of plant cells

  • JPR Symposium
  • Reprogramming of plant cells as adaptive strategies
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Abstract

The characteristic high regenerative ability of plants has been exploited to develop in vitro plant regeneration techniques, which are usually initiated by an in vitro dedifferentiation step induced by artificial phytohormone treatment. Recent advances in plant molecular biological and genetic technologies have revealed the importance of the regulation of RNA metabolism, including the control of rRNA biosynthesis, pre-mRNA splicing, and miRNA-based RNA decay, in successful in vitro dedifferentiation. This review provides a brief overview of current knowledge of the roles of RNA metabolism in the dedifferentiation of plant cells in vitro. In addition, the possibility that plant-specific aspects of RNA metabolism regulation are linked closely to their high regenerative ability is discussed.

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Acknowledgments

I thank Dr. Munetaka Sugiyama (University of Tokyo) for critical discussions and helpful suggestions. I also thank Dr. Dominique Gagliardi and Dr. Heike Lange (Université de Strasbourg) for providing the mtr4 mutants, Dr. Kentaro Nakaminami and Dr. Motoaki Seki (RIKEN Center for Sustainable Resource Science) for providing the xrn mutants, and Dr. Taku Demura (Nara Institute of Science and Technology), Dr. Yuichiro Watanabe (University of Tokyo) and Dr. Hiroyasu Motose (Okayama University) for fruitful discussions. This work was supported in part by a Start-up Grant for Women Researchers from the Nara Institute of Science and Technology and by Grants-in-Aid from the Japan Society for the Promotion of Science (Grant numbers 18870028, 24770052, and 25114520 to M.O.).

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The authors declare that they have no conflict of interest.

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  1. Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara, 630-0192, Japan

    Misato Ohtani

  2. Biomass Engineering Program Cooperation Division, RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045, Japan

    Misato Ohtani

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Ohtani, M. Regulation of RNA metabolism is important for in vitro dedifferentiation of plant cells. J Plant Res 128, 361–369 (2015). https://doi.org/10.1007/s10265-015-0700-4

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