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Identification and Analysis of Muscle-Related Protein Isoforms Expressed in the White Muscle of the Mandarin Fish (Siniperca chuatsi)

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Abstract

To identify muscle-related protein isoforms expressed in the white muscle of the mandarin fish Siniperca chuatsi, we analyzed 5,063 high-quality expressed sequence tags (ESTs) from white muscle cDNA library and predicted the integrity of the clusters annotated to these genes and the physiochemical properties of the putative polypeptides with full length. Up to about 33% of total ESTs were annotated to muscle-related proteins: myosin, actin, tropomyosin/troponin complex, parvalbumin, and Sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCa). Thirty-two isoforms were identified and more than one isoform existed in each of these proteins. Among these isoforms, 14 putative polypeptides were with full length. In addition, about 2% of total ESTs were significantly homologous to “glue” molecules such as alpha-actinins, myosin-binding proteins, myomesin, tropomodulin, cofilin, profilin, twinfilins, coronin-1, and nebulin, which were required for the integrity and maintenance of the muscle sarcomere. The results demonstrated that multiple isoforms of major muscle-related proteins were expressed in S. chuatsi white muscle. The analysis on these isoforms and other proteins sequences will greatly aid our systematic understanding of the high flexibility of mandarin fish white muscle at molecular level and expand the utility of fish systems as models for the muscle genetic control and function.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 30771644; 30972263) and the Nature and Science Foundation of Hunan (09JJ6037). We thank Ying Liu for the technical assistance.

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Authors and Affiliations

  1. Department of Bioengineering & Environmental Science, Changsha University, Changsha, 410003, People’s Republic of China

    Wuying Chu, Tao Meng, Renxue Zhou, Zhen Liu & Jianshe Zhang

  2. Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, People’s Republic of China

    Guoqiang Zhang, Songnian Hu & Linlin Pan

  3. Graduated University of the Chinese Academy of Sciences, Beijing, 100094, People’s Republic of China

    Guoqiang Zhang

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  1. Guoqiang Zhang
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  2. Wuying Chu
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  3. Songnian Hu
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  4. Tao Meng
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  5. Linlin Pan
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  6. Renxue Zhou
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  7. Zhen Liu
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  8. Jianshe Zhang
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Correspondence to Songnian Hu or Jianshe Zhang.

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Guoqing Zhang and Wuying Chu contributed equally to this work.

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Supplemental 1

Supplementary figures (sequence alignments). This file containing alignments of polypeptides putatively deduced from mandrin fish muscle-related genes with other species. (DOC 211 kb)

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Zhang, G., Chu, W., Hu, S. et al. Identification and Analysis of Muscle-Related Protein Isoforms Expressed in the White Muscle of the Mandarin Fish (Siniperca chuatsi). Mar Biotechnol 13, 151–162 (2011). https://doi.org/10.1007/s10126-010-9275-1

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