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Influence of three laser wavelengths on human fibroblasts cell culture

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Abstract

Although experimental studies in vitro and vivo have been numerous, the effect of laser wavelength irradiation on human fibroblast cell culture is poorly understood. This emphasizes the need of additional cellular and molecular research into laser influence with low energy and power. The aim of this study was to assess the influence of three different laser wavelengths on the human skin fibroblasts cell culture. We wanted to evaluate if near infrared lasers had any influence in healing of wounds by stimulating mitochondrial activity of fibroblasts. The cells were irradiated using 830-, 980- and 2,940-nm laser wavelengths. The irradiated cells were incubated and their mitochondrial activity was assessed by the MTT assay at 24, 48 and 72 h. Simultaneously, an apoptosis assay was assessed on the irradiated fibroblasts. It can be concluded that laser light of the near-infrared region (830 and 980 nm) influences fibroblasts mitochondrial activity compared to the 2,940-nm wavelength which produces apoptosis.

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Authors and Affiliations

  1. Department of Implantology and Maxillofacial Surgery, University of Medicine and Pharmacy “Iuliu Hatieganu”, No. 37, Cardinal Iuliu Hossu Street, 400029, Cluj-Napoca, Romania

    Bogdan Crisan & Mihaela Baciut

  2. Oncological Institute “Prof. I. Chiricuta”, No. 2, Ion Creanga Street, 400010, Cluj-Napoca, Romania

    Olga Soritau

  3. Department of Oral Rehabilitation, University of Medicine and Pharmacy “Iuliu Hatieganu”, No. 15, Victor Babes Street, 400012, Cluj-Napoca, Romania

    Radu Campian

  4. Department of Cranio-Maxillofacial Surgery, University of Medicine and Pharmacy “Iuliu Hatieganu”, No. 37, Cardinal Iuliu Hossu Street, 400029, Cluj-Napoca, Romania

    Liana Crisan & Grigore Baciut

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  1. Bogdan Crisan
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  2. Olga Soritau
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  3. Mihaela Baciut
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  4. Radu Campian
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  5. Liana Crisan
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  6. Grigore Baciut
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Correspondence to Bogdan Crisan.

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Crisan, B., Soritau, O., Baciut, M. et al. Influence of three laser wavelengths on human fibroblasts cell culture. Lasers Med Sci 28, 457–463 (2013). https://doi.org/10.1007/s10103-012-1084-5

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  • DOI: https://doi.org/10.1007/s10103-012-1084-5

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