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Effects of exercise after focal cerebral cortex infarction on basal ganglion

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Abstract

Identification of functional molecules in the brain related to improvement of motor dysfunction after stroke will contribute to establish a new treatment strategy for stroke rehabilitation. Hence, monoamine changes in basal ganglion related to motor control were examined in groups with/without voluntary exercise after cerebral infarction. Cerebral infarction was produced by photothrombosis in rats. Voluntary exercise using a running wheel was initiated from 2 days after surgery. Motor performance was measured by the accelerated rotarod test. Monoamine concentrations in striatum were analyzed using HPLC and immunohistochemical staining performed with anti-tyrosine hydroxylase antibody. In behavioral evaluation, the mean latency until falling from the rotating rod in the group with exercise (infarction-EX group) was significantly longer than that in the group without exercise (infarction-CNT group). When concerning the alteration of monoamine concentration between before and 2 days after infarction, dopamine level showed a significant increase 2 days after infarction. Subsequently, dopamine level was significantly decreased in the infarction-EX group at 10 days after infarction; in contrast, both norepinephrine and 5-HT concentrations were significantly higher in the infarction-EX group than in the infarction-CNT group. Furthermore, duration of rotarod test showed a significant inverse correlation with dopamine levels and a significant positive correlation with 5-HT levels. In immunohistochemical analysis, tyrosine hydroxylase immunoreactivity in substantia nigra pars compacta was shown to increase in the infarction-CNT group. In the present study, at least some of the alterations of monoamines associated with the improvement of paralysis in the basal ganglion related to motor control might have been detected.

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Acknowledgments

This study was supported by a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology of Japan [KAKENHI] No. 23700642.

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The authors declare that they have no competing financial interests.

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Authors and Affiliations

  1. Division of Biochemistry, Fujita Memorial Nanakuri Institute, Fujita Health University, 1865 Hisai-isshiki-cho, Tsu, Mie, 514-1296, Japan

    Kenmei Mizutani, Shigeru Sonoda, Kan Shimpo & Takeshi Chihara

  2. Seijoh University Graduate School of Health Care Studies, Aichi, Japan

    Nobuyuki Karasawa, Terumi Takeuchi, Yoko Hasegawa & Kin-ya Kubo

  3. Department of Anatomy, Faculty of Medical Technology, School of Health Sciences, Fujita Health University, Aichi, Japan

    Keiki Yamada

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  1. Kenmei Mizutani
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  2. Shigeru Sonoda
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  3. Nobuyuki Karasawa
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  4. Keiki Yamada
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  5. Kan Shimpo
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  6. Takeshi Chihara
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  7. Terumi Takeuchi
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  8. Yoko Hasegawa
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  9. Kin-ya Kubo
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Correspondence to Kenmei Mizutani.

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Mizutani, K., Sonoda, S., Karasawa, N. et al. Effects of exercise after focal cerebral cortex infarction on basal ganglion. Neurol Sci 34, 861–867 (2013). https://doi.org/10.1007/s10072-012-1137-3

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  • DOI: https://doi.org/10.1007/s10072-012-1137-3

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