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Antioxidant activities of aqueous extracts from three cultivars of guava leaf

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Abstract

In order to obtain basic data required for utilization of guava leaf as a functional substance, the antioxidant activities of aqueous extracts from 3 cultivars of guava leaf (‘Apple color’, ‘Ruby’, and ‘Safeda’) were examined. The total phenolic contents of the aqueous extracts ranged from 257.38 to 293.25 mg/g gallic acid equivalents. DPPH, ABTS, reducing power, ferric reducing antioxidant power (FRAP), ferric thiocyanate (FTC), and malondialdehyde (MDA) assays indicated that the aqueous extract of the ‘Ruby’ cultivar was the most potent radicalscavenger and reducing agent compared to the other 2 cultivars. Therefore, this study verified that aqueous extract from the ‘Ruby’ cultivar possessed strong antioxidant activity that correlated to its high level of phenolics, particularly gallic acid. In conclusion, the aqueous extract of the ‘Ruby’ cultivar of guava leaf may be utilized as an effective source of functional food materials, including natural antioxidants.

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References

  1. Singh A. Chemical and biochemical aspects of activated oxygen: Singlet oxygen, superoxide anion, and related species. Vol. I, pp. 17–28. In: Handbook of Free Radical and Antioxidant in Biomedicine. Miquel J, Quintaanilha AT, Weber H (eds). CRC Press, Inc., Boca Raton, FL, USA (1989)

    Google Scholar 

  2. Aruoma O. Assessment of potential prooxidant and antioxidant actions. J. Am. Oil Chem. Soc. 73: 1617–1625 (1996)

    Article  CAS  Google Scholar 

  3. Sawa T, Akaike T, Maeda H. Tyrosine nitration by peroxynitrite formed from nitric oxide and superoxide generated by xanthine oxidase. J. Biol. Chem. 275: 32467–32474 (2000)

    Article  CAS  Google Scholar 

  4. Choi HR, Choi JS, Han YN, Bae SJ, Chung HY. Peroxynitrite scavenging activity of herb extracts. Phytother. Res. 16: 364–367 (2002)

    Article  CAS  Google Scholar 

  5. Squadrito GL, Pryor WA. Oxidative chemistry of nitric oxide: The roles of superoxide, peroxynitrite, and carbon dioxide. Free Radical Bio. Med. 25: 392–403 (1998)

    Article  CAS  Google Scholar 

  6. Cao G, Sofic E, Prior RL. Antioxidant capacity of tea and common vegetables. J. Agr. Food Chem. 44: 3426–3431 (1996)

    Article  CAS  Google Scholar 

  7. Branen A. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52: 59–63 (1975)

    Article  CAS  Google Scholar 

  8. Joseph B, Priya M. Review on nutritional, medicinal, and pharmacological properties of guava (Psidium Guajava Linn.). Int. J. Pharma. Bio. Sci. 2: 53–69 (2011)

    Google Scholar 

  9. Morton JF. Fruits of Warm Climates. Julia F Morton, Miami, FL, USA. pp. 356–363. (1987)

    Google Scholar 

  10. Oh WK, Lee CH, Lee MS, Bae EY, Sohn CB, Oh H, Kim BY, Ahn JS. Antidiabetic effects of extracts from Psidium guajava. J. Ethnopharmacol. 96: 411–415 (2005)

    Article  Google Scholar 

  11. Okuda T, Yoshida T, Hatano T, Yazaki K, Ikegami Y, Shingu T. Guavins A, C, and D, complex tannins from Psidium guajava. Chem. Pharm. Bull. 35: 443–446 (1987)

    Article  CAS  Google Scholar 

  12. Begum S, Hassan SI, Siddiqui BS, Shaheen F, Nabeel Ghayur M, Gilani AH. Triterpenoids from the leaves of Psidium guajava. Phytochemistry 61: 399–403 (2002)

    Article  CAS  Google Scholar 

  13. Meckes M, Calzada F, Tortoriello J, González JL, Martínez M. Terpenoids isolated from Psidium guajava hexane extract with depressant activity on central nervous system. Phytother. Res. 10: 600–603 (1996)

    Article  CAS  Google Scholar 

  14. Lozoya X, Meckes M, Abou-Zaid M, Tortoriello J, Nozzolillo C, Arnason JT. Quercetin glycosides in Psidium guajava L. leaves and determination of a spasmolytic principle. Arch. Med. Res. 25: 11–15 (1994)

    CAS  Google Scholar 

  15. Tanaka T, Ishida N, Ishimatsu M, Nonaka G, Nishioka I. Tannins and related compounds. CXVI. Six new complex tannins, guajavins, psidinins, and psiguavin from the bark of Psidium guajava L. Chem. Pharm. Bull. 40: 2092–2098 (1992)

    Article  CAS  Google Scholar 

  16. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal. 19: 669–675 (2006)

    Article  CAS  Google Scholar 

  17. Jaiarj P, Khoohaswan P, Wongkrajang Y, Peungvicha P, Suriyawong P, Sumal Saraya ML, Ruangsomboon O. Anticough and antimicrobial activities of Psidium guajava Linn. leaf extract. J. Ethnopharmacol. 67: 203–212 (1999)

    Article  CAS  Google Scholar 

  18. Hsieh CL, Lin YC, Yen GC, Chen HY. Preventive effects of guava (Psidium guajava L.) leaves and its active compounds against α-dicarbonyl compounds-induced blood coagulation. Food Chem. 103: 528–535 (2007)

    Article  CAS  Google Scholar 

  19. Wang H, Du YJ, Song HC. α-Glucosidase and α-amylase inhibitory activities of guava leaves. Food Chem. 123: 6–13 (2010)

    Article  CAS  Google Scholar 

  20. Chen HY, Yen GC. Antioxidant activity and free radical-scavenging capacity of extracts from guava (Psidium guajava L.) leaves. Food Chem. 101: 686–694 (2007)

    Article  CAS  Google Scholar 

  21. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199–1200 (1958)

    Article  CAS  Google Scholar 

  22. Fellegrini N, Ke R, Yang M, Rice-Evans C. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid radical cation decolorization assay. pp. 379–389. In: Methods in Enzymology. Lester P (ed). Academic Press, New York, NY, USA (1999)

    Google Scholar 

  23. Oyaizu M. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44: 307–315 (1986)

    Article  CAS  Google Scholar 

  24. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem. 239: 70–76 (1996)

    Article  CAS  Google Scholar 

  25. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from acacia confusa bark and heartwood. J. Agr. Food Chem. 49: 3420–3424 (2001)

    Article  CAS  Google Scholar 

  26. Jeong CH, Jeong HR, Choi SG, Shim KH, Heo HJ. Neuronal cell protection and antioxidant activities of hot water extract from commercial buckwheat tea. Korean J. Food Preserv. 18: 358–365 (2011)

    Google Scholar 

  27. Pyo YH, Lee TC, Logendra L, Rosen RT. Antioxidant activity and phenolic compounds of Swiss chard (Beta vulgaris subspecies cycla) extracts. Food Chem. 85: 19–26 (2004)

    Article  CAS  Google Scholar 

  28. Awika JM, Rooney LW, Wu X, Prior RL, Cisneros-Zevallos L. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J. Agr. Food Chem. 51: 6657–6662 (2003)

    Article  CAS  Google Scholar 

  29. Sevanian A, Ursini F. Lipid peroxidation in membranes and lowdensity lipoproteins: Similarities and differences. Free Radical Bio. Med. 29: 306–311 (2000)

    Article  CAS  Google Scholar 

  30. Kim DO, Lee KW, Lee HJ, Lee CY. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J. Agr. Food Chem. 50: 3713–3717 (2002)

    Article  CAS  Google Scholar 

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Correspondence to Sun Jin Hur.

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Jeong, C.H., Bae, Y.I., Park, S.J. et al. Antioxidant activities of aqueous extracts from three cultivars of guava leaf. Food Sci Biotechnol 21, 1557–1563 (2012). https://doi.org/10.1007/s10068-012-0207-x

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