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Transcriptional alteration of p53 related processes as a key factor for skeletal muscle characteristics in Sus scrofa

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Molecules and Cells

Abstract

The pig could be a useful model to characterize molecular aspects determining several delicate phenotypes because they have been bred for those characteristics. The Korean native pig (KNP) is a regional breed in Korea that was characterized by relatively high intramuscular fat content and reddish meat color compared to other western breeds such as Yorkshire (YS). YS grew faster and contained more lean muscle than KNP. We compared the KNP to Yorksire to find molecular clues determining muscle characteristics. The comparison of skeletal gene expression profiles between these two breeds showed molecular differences in muscle. We found 82 differentially expressed genes (DEGs) defined by fold change (more than 1.5 fold difference) and statistical significance (within 5% of false discovery rate). Functional analyses of these DEGs indicated up-regulation of most genes involved in cell cycle arrest, down-regulation of most genes involved in cellular differentiation and its inhibition, down-regulation of most genes encoding component of muscular-structural system, and up-regulation of most genes involved in diverse metabolism in KNP. Especially, DEGs in above-mentioned categories included a large number of genes encoding proteins directly or indirectly involved in p53 pathway. Our results indicated a possible role of p53 to determine muscle characteristics between these two breeds.

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Authors and Affiliations

  1. College of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    Seung-Soo Kim, Jung-Rok Kim, Jin-Kyoo Moon & Cheol-Koo Lee

  2. National Institute of Animal Science, Rural Development Administration, Suwon, 441-706, Korea

    Bong-Hwan Choi & Tae-Hun Kim

  3. Department of Animal Science, Chungbuk National University, Cheongju, 361-763, Korea

    Kwan-Suk Kim

  4. School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Korea

    Jong-Joo Kim

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  1. Seung-Soo Kim
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  2. Jung-Rok Kim
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Kim, SS., Kim, JR., Moon, JK. et al. Transcriptional alteration of p53 related processes as a key factor for skeletal muscle characteristics in Sus scrofa . Mol Cells 28, 565–573 (2009). https://doi.org/10.1007/s10059-009-0159-z

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  • DOI: https://doi.org/10.1007/s10059-009-0159-z

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