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Insulin-like growth factor-I induces androgen receptor activation in differentiating C2C12 skeletal muscle cells

  • Published:
Molecules and Cells

Abstract

The modulating effect of IGF-I on the regulation of AR gene expression and activation in skeletal muscle cells remains poorly understood. In this study, the effects of IGF-I treatment on AR induction and activation in the absence of AR ligands were examined. Differentiating C2C12 cells were treated with different concentrations (0–250 ng/ml) of IGF-I or for various periods of time (0–60 min) of 250 ng/ml IGF-I. Treatment of C2C12 cells with IGF-I resulted in a dose- and time-dependent increase in total AR and phosphorylated AR (Ser 213). IGF-I treatment also led to significantly increased AR mRNA expression when compared with the control. The levels of skeletal α-actin and myogenin mRNA, known target genes of AR, were also significantly upregulated after 5 or 10 min of treatment with IGF-I. Confocal images revealed that IGF-I stimulated nuclear localization of AR in the absence of ligands. In addition, an electrophoretic mobility shift assay indicated that IGF-I stimulated the AR DNA binding activity in a time-dependent manner. The present results suggest that IGF-I stimulates the expression and activation of AR by ligand-independent mechanism in differentiating C2C12 mouse skeletal muscle cells.

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Authors and Affiliations

  1. Department of Exercise Science, College of Health Sciences, Ewha Womans University, Seoul, 120-750, Korea

    Hye Jin Kim & Won Jun Lee

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  1. Hye Jin Kim
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  2. Won Jun Lee
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Correspondence to Won Jun Lee.

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Kim, H.J., Lee, W.J. Insulin-like growth factor-I induces androgen receptor activation in differentiating C2C12 skeletal muscle cells. Mol Cells 28, 189–194 (2009). https://doi.org/10.1007/s10059-009-0118-8

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  • DOI: https://doi.org/10.1007/s10059-009-0118-8

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