Skip to main content
SpringerLink
Log in

Isoptericola salitolerans sp. nov., a halotolerant filamentous actinobacterium isolated from a salt lake, China

  • Original Paper
  • Published:
Extremophiles Aims and scope Submit manuscript

Abstract

A novel bacterial strain, TRM F109T, was isolated from hypersaline habitat in Sichuan Province, China. It was a Gram-positive, aerobic, non-motile, halotolerant, filamentous bacterium. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0–98.4 % to the type strains of recognized species of the genus Isoptericola. Chemotaxonomic data also supported the placement of strain TRM F109T within the genus Isoptericola. The low levels of DNA–DNA relatedness between the novel strain and the type strains of recognized species of the genus Isoptericola, in combination with differential phenotypic data, demonstrate that strain TRM F109T represents a novel species of the genus Isoptericola, for which the name Isoptericola salitolerans sp. nov. is proposed. The type strain is TRM F109T (=JCM 15901T=KCTC 19617T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bakalidou A, Kämpfer P, Berchtold M, Kuhnigk T, Wenzel M, König H (2002) Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis. Int J Syst Evol Microbiol 5:1185–1192

    Article  Google Scholar 

  • Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    Article  PubMed  CAS  Google Scholar 

  • Cui XL, Mao PH, Tseng M, Li WJ, Zhang LP, Xu LH, Jiang CL (2001) Streptomonospora salina gen. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51:357–363

    PubMed  CAS  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789

    Article  Google Scholar 

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC

    Google Scholar 

  • Gordon RE, Barnett DA, Handerhan JE, Pang CHN (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63

    Article  Google Scholar 

  • Groth I, Schumann P, Rainey FA, Martin K, Schuetze B, Augsten K (1997) Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133

    Article  PubMed  CAS  Google Scholar 

  • Groth I, Schumann P, Schütze B, Gonzalez JM, Laiz L, Cesareo SJ, Stackebrandt E (2005) Isoptericola hypogeus sp. nov., isolated from the Roman catacomb of Domitilla. Int J Syst Evol Microbiol 55:1715–1719

    Article  PubMed  CAS  Google Scholar 

  • Guan TW, Zhao K, Xiao J, Liu Y, Xia ZF, Zhang XP, Zhang LL (2010) Brevibacterium salitolerans sp. nov., an actinobacterium isolated from a salt lake in Xinjiang, China. Int J Syst Evol Microbiol 60:2991–2995

    Article  PubMed  CAS  Google Scholar 

  • He L, Li W, Huang Y, Wang L, Liu Z, Lanoot B, Vancanneyt M, Swings J (2005) Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55:1939–1944

    Article  PubMed  CAS  Google Scholar 

  • Huang Z, Sheng XF, Zhao F, He LY, Huang J, Wang Q (2012) Isoptericola nanjingensis sp. nov., a mineral-weathering bacterium. Int J Syst Evol Microbiol 62:971–976

    Article  PubMed  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120

    Article  PubMed  CAS  Google Scholar 

  • Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    Article  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233

    Article  CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. IDI Inc., Newark

    Google Scholar 

  • Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477

    PubMed  CAS  Google Scholar 

  • Stackebrandt E, Rainey FA, Ward-Rainey NL (1997) Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491

    Article  Google Scholar 

  • Stackebrandt E, Schumann P, Cui XL (2004) Reclassification of Cellulosimicrobium variabile Bakalidou et al. 2002 as Isoptericola variabilis gen. nov., comb. nov. Int J Syst Evol Microbiol 54:685–688

    Article  PubMed  CAS  Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:226–231

    PubMed  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  • Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJ (2009) Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 59:2025–2032

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  • Tseng M, Liao HC, Chiang WP, Yuan GF (2011) Isoptericola chiayiensis sp. nov., isolated from mangrove soil. Int J Syst Evol Microbiol 61:1667–1670

    Article  PubMed  CAS  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Wu Y, Li WJ, Tian W, Zhang LP, Xu L, Shen Q, Shen B (2010) Isoptericola jiangsuensis sp. nov., a chitin-degrading bacterium. Int J Syst Evol Microbiol 60:904–908

    Article  PubMed  CAS  Google Scholar 

  • Yoon JH, Schumann P, Kang SJ, Jung SY, Oh TK (2006) Isoptericola dokdonensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:2893–2897

    Article  PubMed  CAS  Google Scholar 

  • Zhang YQ, Schumann P, Li WJ, Chen GZ, Tian XP, Stackebrandt E, Xu LH, Jiang CL (2005) Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China. Int J Syst Evol Microbiol 55:1867–1870

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by National Natural Science Foundation of China (Project No. 31060003), Key Research Foundation of Xihua University (Z1220530), Key Project of Office of Education of Sichuan Province, China (Project No. 2013) and Key Project for Department of Science and Technology of Ministry of Education of China (Project No. 209145).

Author information

Authors and Affiliations

  1. School of Bioengineering, Xihua University, Chengdu, 610039, People’s Republic of China

    Tong-Wei Guan & Zhen-Ming Che

  2. Faculty of Resource and Environmental Sciences, Sichuan Agricultural University, Yaan, 625000, People’s Republic of China

    Yun Teng & Xiao-Ping Zhang

  3. The Key Laboratory for Microbial Resources of the Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Ling-Ling Yang

Authors
  1. Tong-Wei Guan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yun Teng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ling-Ling Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiao-Ping Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhen-Ming Che
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tong-Wei Guan.

Additional information

Communicated by A. Oren.

The 16S rRNA gene sequence of strain TRM F109T has been deposited in GenBank under the accession number JX453452.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 83 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guan, TW., Teng, Y., Yang, LL. et al. Isoptericola salitolerans sp. nov., a halotolerant filamentous actinobacterium isolated from a salt lake, China. Extremophiles 17, 471–476 (2013). https://doi.org/10.1007/s00792-013-0531-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00792-013-0531-y

Keywords

Search

Navigation