Antioxidant effects of the VO(IV) hesperidin complex and its role in cancer chemoprevention

  • Susana Beatriz Etcheverry
  • Evelina Gloria Ferrer
  • Luciana Naso
  • Josefina Rivadeneira
  • Victoria Salinas
  • Patricia Ana María Williams
Original Paper

DOI: 10.1007/s00775-007-0332-9

Cite this article as:
Etcheverry, S.B., Ferrer, E.G., Naso, L. et al. J Biol Inorg Chem (2008) 13: 435. doi:10.1007/s00775-007-0332-9

Abstract

Vanadium compounds are known for a variety of pharmacological properties. Many of them display antitumoral and osteogenic effects in several cell lines. Free radicals induce the development of tumoral processes. Natural polyphenols such as flavonoids have antioxidant properties since they scavenge different free radicals. For these reasons it is interesting to investigate the effects of a new complex generated between the vanadyl(IV) cation and the flavonoid hesperidin. The complex has been synthesized and characterized by physicochemical methods. Spectroscopic analysis revealed a 1:1 stoichiometry of ligand:VO and coordination by deprotonated cis-hydroxyl groups to the disaccharide moiety of the ligand. The complex improves the superoxide dismutase (SOD)-like activity of the ligand, but the scavenging of other radicals tested does not change upon complexation. When tested on two tumoral cell lines in culture (one of them derived from a rat osteosarcoma UMR106 and the other from human colon adenocarcinoma Caco-2), the complex enhanced the antiproliferative effects of the free ligand, and this effect correlated with the morphological alterations toward apoptosis. Also, on the osteoblastic cell line the complex stimulated cell proliferation and collagen type I production at low concentrations. At higher doses the complex behaved as a cytotoxic compound for the osteoblasts.

Keywords

Antioxidants Antitumoral Vanadium hesperidin complex Cellular morphology 

Abbreviations

ABTS

2,2′-Azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid diammonium salt)

ALP

Alkaline phosphatase

DMEM

Dulbecco’s modified Eagles medium

DPPH·

1,1-Diphenyl-2-picrylhydrazyl radical

EDTA

Ethylenediaminetetraacetic acid

NADH

Nicotinamide adenine dinucleotide

NBT

Nitroblue tetrazolium

PMS

Phenazine methosulfate

p-NP

p-Nitrophenol

p-NPP

p-Nitrophenylphosphate

SOD

Superoxide dismutase

TEAC

Trolox-equivalent antioxidant coefficient

Trolox

6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

Copyright information

© SBIC 2007

Authors and Affiliations

  • Susana Beatriz Etcheverry
    • 1
    • 2
  • Evelina Gloria Ferrer
    • 1
  • Luciana Naso
    • 1
  • Josefina Rivadeneira
    • 1
    • 2
  • Victoria Salinas
    • 1
  • Patricia Ana María Williams
    • 1
  1. 1.Centro de Química Inorgánica (CEQUINOR/CONICET,UNLP), Facultad de Ciencias ExactasUniversidad Nacional de La PlataLa PlataArgentina
  2. 2.Cátedra de Bioquímica Patológica, Facultad de Ciencias ExactasUniversidad Nacional de La PlataLa PlataArgentina

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