Skip to main content
SpringerLink
Log in

Vanadium derivatives act as growth factor — mimetic compounds upon differentiation and proliferation of osteoblast-like UMR106 cells

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

The effect of different vanadium compounds on proliferation and differentiation was examined in osteoblast-like UMR106 cells. Vanadate increased the cell growth in a biphasic manner, the higher doses inhibiting cell progression. Vanadyl stimulated cell proliferation in a dose-responsive manner. Similar to vanadate, pervanadate increased osteoblast-like cell proliferation in a biphasic manner but no inhibition of growth was observed. Vanadyl and pervanadate were stronger stimulators of cell growth than vanadate. Only vanadate was able to regulate the cell differentiation as measured by cell alkaline phosphatase activity. These results suggest that vanadium derivatives behave like growth factors on osteoblast-like cells and are potential pharmacological tools in the control of cell growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Compston JE: Osteoporosis. Clin Endocrinol 33: 653–682, 1990

    Google Scholar 

  2. Marcus R: Skeletal aging. Understanding the functional and structural bases of osteoporosis. Trends Endocrinol Metab 2: 53–58, 1991

    Google Scholar 

  3. Cooper C, Melton J II: Epidemiology of osteoporosis. Trends Endocrinol Metab. 3: 224–229, 1992

    Google Scholar 

  4. Farley JR, Tarbaux N, Murphy LA, Masuda T, Baylink DJ:In vitro evidence that bone formation may be coupled to bone resorption by release of mitogen(s) from resorbing bone. Metabolism 36: 314–321, 1987

    Google Scholar 

  5. Baylink DJ, Finkelman RD, Mohan S: Growth factors to stimulate bone formation. J Bone Min Res 8, Supp 2: S565-S572, 1993

    Google Scholar 

  6. Centrella M, Canalis E: Local regulators of skeletal growth: a perspective. Endocr Rev 6: 544–551, 1985

    Google Scholar 

  7. Levy JR, Murray E, Manolagas S, Olefky JM: Demonstration of insulin receptors and modulation of alkaline phosphatase activity by insulin in rat osteoblastic cells. Endocrinology 119: 1786–1792, 1986

    Google Scholar 

  8. Mohan S, Baylink DJ: The role of insulin-like growth factor-II in the coupling of bone formation to resorption. In: E.M. Spencer (ed.). Modern Concepts in Insulin-like Growth Factors. Elsevier, New York, 1991, pp. 169–184

    Google Scholar 

  9. Hashizume M, Yamaguchi M: Stimulatory effect of β-alanyl-L-histidinato zinc on cell proliferation is dependent on protein synthesis in osteoblastic MC3T3-E1 cells. Mol Cell Biochem 122: 59–64, 1993

    Google Scholar 

  10. Nielsen FH, Uthus EC: The essentiality and metabolism of vanadium. In: N.D. Chasteen (ed.). Vanadium in Biological Systems. Kluwer Academic Publishers, Netherlands, 1990, pp. 51–62

    Google Scholar 

  11. Canalis E: Effect of sodium vanadate on deoxyribonucleic acid and protein synthesis in cultured rat calvariae. Endocrinology 116: 855–862, 1985

    Google Scholar 

  12. Kato Y, Iwamoto M, Koke T, Suzuki F: Effect of vanadate on cartilage-matrix proteoglycan synthesis in rabbit costal chondrocyte cultures. J Cell Biol 104: 311–319, 1987

    Google Scholar 

  13. Shechter Y, Amir S, Meyerovitch J: The insulin-like metabolic effects of vanadate: possible implications to the future care of diabetes. Diab Nutr Metab 1: 1–5, 1988

    Google Scholar 

  14. Fantus IG, Kadota S, Deragon G, Foster B, Posner BI: Pervanadate [Peroxide(s) of vanadate] mimics insulin action in rat adipocytes via activation of the insulin receptor tyrosine kinase. Biochemistry 28: 8864–8871, 1989

    Google Scholar 

  15. Trudel S, Paquet MR, Grinstein S: Mechanism of vanadate-induced activation of tyrosine phosphorylation and of the respiratory burst in HL60 cells. Role of reduced oxygen metabolites. Biochem J 276: 611–619, 1991

    Google Scholar 

  16. Davidai G, Lee A, Schuartz I, Hazum E: PDGF induces tyrosine phosphorylation in osteoblast-like cells: relevance to mitogenesis. Am J Physiol 263: E205-E209, 1992

    Google Scholar 

  17. Shisheva A, Shechter Y: Mechanism of pervanadate stimulation and potentiation of insulin-activated glucose transport in rat adipocytes. Dissociation from vanadate effect. Endocrinology 133: 1562–1568, 1993

    Google Scholar 

  18. Cortizo AM, Salice VC, Etcheverry SB: Vanadium compounds: their action on alkaline phosphatase activity. Biological Trace Element Research 41: 331–339, 1994

    Google Scholar 

  19. Partridge NC, Alcorn D, Michelangeli VP, Ryan G, Martin TJ: Morphological and biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin. Cancer Res 43: 4308–4314, 1983

    Google Scholar 

  20. Okajima T, Nakamura K, Zhang H, Ling N, Tanabe T, Yasuda T, Rosenfeld RG: Sensitive colorimetric bioassays for insulin-like growth factor (IGF) stimulation of cell proliferation and glucose consumption: use in studies of IGF analogs. Endocrinology 130: 2201–2212, 1992

    Google Scholar 

  21. Bradford M: Rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254, 1976

    Google Scholar 

  22. Stein GS, Lian JB: Molecular mechanism mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. Endocrine Rev 14: 424–442, 1993

    Google Scholar 

  23. Schmid CH, Steiner TH, Froesch ER: Insulin-like growth factors stimulate synthesis of nucleic acids and glycogen in cultured calvaria cells. Calcif Tissue Int 35: 578–585, 1983

    Google Scholar 

  24. Bonewald LF, Schwartz Z, Swain LD, Ramirez V, Poser J, Boyan BD: Stimulation of plasma membrane and matrix vesicle enzyme activity by transforming growth factor-β in osteosarcoma cell cultures. J Cell Physiol 145: 200–206, 1990

    Google Scholar 

  25. Zhang RW, Simmons DJ, Crowther RS, Mohan S, Baylink DJ: Contribution of marrow stroma cells to the regulation of osteoblast proliferation in rats: evidence for the involvement of insulin-like growth-factors. Bone and Mineral 13: 201–215, 1991

    Google Scholar 

  26. Crans DC, Bunch RL, Theisen LA: Interaction of trace levels of vanadium (IV) and vanadium (V) in biological systems. J Am Chem Soc 111: 7597–7607, 1989

    Google Scholar 

  27. Kadota S, Fantus IG, Deragon G, Guyda HJ, Hersh B, Posner BI: Peroxide(s) of vanadium: a novel and potent insulin-mimetic agent which activates the insulin receptor kinases. Biochem Biophys Res Comm 147: 259–263, 1987

    Google Scholar 

  28. Yarden Y, Ullrich A: Growth factor receptor tyrosine kinases. Ann Rev Biochem 57: 443–478, 1988

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, (1900), La Plata, Argentina

    Ana M. Cortizo & Susana B. Etcheverry

  2. Programa QUINOR, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, (1900), La Plata, Argentina

    Susana B. Etcheverry

Authors
  1. Ana M. Cortizo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Susana B. Etcheverry
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cortizo, A.M., Etcheverry, S.B. Vanadium derivatives act as growth factor — mimetic compounds upon differentiation and proliferation of osteoblast-like UMR106 cells. Mol Cell Biochem 145, 97–102 (1995). https://doi.org/10.1007/BF00935481

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00935481

Key words

Search

Navigation