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RETRACTED ARTICLE: Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway

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Journal of Bone and Mineral Metabolism Aims and scope Submit manuscript

This article was retracted on 03 February 2022

A Correction to this article was published on 08 June 2020

This article has been updated

Abstract

Osteosarcoma (OS) is a prevalent cancer that plagues people worldwide. Identifying prognostic markers would be useful in treating human OS. In this study, we aimed to explore the functions of Ras-related protein Rab-31 (RAB31) in OS-cell proliferation, migration, and invasion as well as its roles in the Hedgehog signaling pathway for better understanding of the mechanism. To assess the detailed regulatory mechanism of RAB31 silencing on OS, both RT-qPCR and Western blot analysis were employed to evaluate the expressions of RAB31 as well as the Hedgehog signaling pathway-related genes. Besides, we also investigated the effects of silenced RAB31 both in vitro and in vivo. First, we found that in OS tissues, both mRNA and protein expressions of RAB31 and PCNA had a significant increase. Second, the Hedgehog signaling pathway was detected to play an integral role in OS progression. Finally, after transfection of RAB31-siRNA to reduce the expression of RAB31, the Hedgehog signaling pathway was suppressed, along with cell proliferation, invasion, and migration. Therefore, we conclude that RAB31 plays an important role in OS development and its silencing delays the OS progression via suppression of the Hedgehog signaling pathway.

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Acknowledgements

We thank the reviewers for critical comments.

Author information

Author notes

  1. Qiong Yu and Dong Li contributed equally to this work.

Authors and Affiliations

  1. Department of Hematology and Oncology, The Second Hospital of Jilin University, No. 218, Ziqiang Street, Changchun, 130041, Jilin, People’s Republic of China

    Qiong Yu, Chun-Mei Hu, Yan Sun, Yan Tang & Guang Shi

  2. Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, 130041, People’s Republic of China

    Dong Li

  3. Department of Breast Surgery, The Second Hospital of Jilin University, Changchun, 130041, People’s Republic of China

    Dan Wang

Authors
  1. Qiong Yu
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  2. Dong Li
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  3. Dan Wang
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  4. Chun-Mei Hu
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  5. Yan Sun
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  6. Yan Tang
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  7. Guang Shi
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Corresponding author

Correspondence to Guang Shi.

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The authors declares that they have conflict of interest.

Ethical approval

All aspects of the study are in strict accordance with the Declaration of Helsinki. All of the above specimens were collected with informed consent of patients and all the patients signed informed consent.

Additional information

This article has been retracted. Please see the retraction notice for more detail: https://doi.org/10.1007/s00774-022-01316-8

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Yu, Q., Li, D., Wang, D. et al. RETRACTED ARTICLE: Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway. J Bone Miner Metab 37, 594–606 (2019). https://doi.org/10.1007/s00774-018-0961-9

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  • DOI: https://doi.org/10.1007/s00774-018-0961-9

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