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Ovariectomy-Induced Osteoporosis Evaluated by 1H One- and Two-Dimensional NMR Transverse Relaxometry

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Abstract

The aim of this work is to show that the bones with marrow, treated as a quasi-porous media, can be successfully used to study the effects of ovariectomy-induced osteoporosis. Proton one-dimensional (1D) nuclear magnetic resonance (NMR) T 2-distribution and two-dimensional (2D) T 2–T 2 exchange maps combined with histological images were used to measure the proximal part of the femoris, diaphysis and distal epiphysis of ovariectomized and non-ovariectomized Wistar albino rats. The 1D normalized T 2 distributions showed four peaks which were associated with protons in four major pools: (1) the protons from bounded water to collagenous matrix; (2) fluids in osteocyte lacunae and canaliculi channels; (3) fluids in secondary pores like Haversian and transverse Volkmann canals and (4) soft matter like bone marrow and fluids in primary pores like trabecular bone cavities. The peak’s association and hierarchical structure of pores in femoral bone were supported by a 2D T 2–T 2 exchange map and by a series of dehydration experiments monitored by NMR measurements. The bone marrow narrows the T 2-distributions, increasing resolution, but will not influence significantly the peaks positions; therefore, the NMR relaxometry is a valuable tool to characterize the pore distributions and effects of induced osteoporosis in diverse bones sections.

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Acknowledgments

This work was supported by a grant of Romanian National Authority for Scientific Research, CNCS-UEFISCDI, Project Number PN-II-IDEI-307/2011. R. F. would like to acknowledge Prof. Bernhard Blümich for useful discussions.

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Authors and Affiliations

  1. University of Medicine and Pharmacy, Gh. Marinescu, 38, 540139, Tg-Mures, Romania

    R. S. Şipos, I. Şuş & Z. Pávai

  2. Department of Physics and Chemistry, Technical University of Cluj-Napoca, 28 Memorandumului Street, 400114, Cluj-Napoca, Romania

    R. Fechete, D. Moldovan & D. E. Demco

  3. DWI-Leibniz Institute for Interactive Materials, RWTH-Aachen University, e.V. Forckenbeckstraße 50, 52056, Aachen, Germany

    D. E. Demco

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  1. R. S. Şipos
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Correspondence to R. Fechete.

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Şipos, R.S., Fechete, R., Moldovan, D. et al. Ovariectomy-Induced Osteoporosis Evaluated by 1H One- and Two-Dimensional NMR Transverse Relaxometry. Appl Magn Reson 47, 1419–1437 (2016). https://doi.org/10.1007/s00723-016-0839-8

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  • DOI: https://doi.org/10.1007/s00723-016-0839-8

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