Abstract
In order to corroborate the hypothesis that variations in the rate of spontaneous ultra-weak photon emission (UPE) from germinating seedlings are related to local variations of the lunisolar tidal force, a series of simultaneous tests was performed using the time courses of UPE collected from three plant species—corn, wheat and sunflower—and also from wheat samples whose grains were transported between continents, from Brazil to The Netherlands and vice versa. All tests which were run in parallel showed coincident inflections within the UPE time courses not only between seedlings of the same species but also between the different species. In most cases, the UPE inflections were synchronised with the turning points in the local gravimetric tidal variation. Statistical tests using the local Pearson correlation verified these coincidences in the two time series. The results therefore support the hypothesis of a relationship between UPE emissions and, in the oscillations, the local gravimetric tide. This applies to both the emissions from seedlings of different species and to the seedlings raised from transported grain samples of the same species.
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References
Barlow PW (1998) Gravity and developmental plasticity. Adv Space Res 21(8/9):1097–1102
Barlow PW (2012) The primal integrated realm and the derived interactive realm in relation to biosemiosis, and their link with the ideas of J.W. von Goethe. Comm Integr Biol 5:434–439
Barlow PW (2015) Leaf movements and their relationship with the lunisolar gravitational force. Ann Bot 116:149–187. doi:10.1093/aob/mcv096
Barlow PW, Fisahn J (2012) Lunisolar tidal force and the growth of plant roots, and some other of its effects on plant movements. Ann Bot 110:301–318. doi:10.1093/aob/mcs038
Barlow PW, Mikulecký M, Střeštík J (2010) Tree-stem diameter fluctuates with the lunar tides and perhaps with geomagnetic activity. Protoplasma 247:25–43. doi:10.1007/s00709-010-01-0136-6
Barlow PW, Fisahn J, Yazdanbakhsh N, Moraes TA, Khabarova OV, Gallep CM (2013) Arabidopsis thaliana root elongation growth is sensitive to lunisolar tidal acceleration and may also be weakly correlated with geomagnetic variations. Ann Bot 111:859–872. doi:10.1093/aob/mct089
Bertogna E, Santos SR, Paterniani JES, Conforti E, Gallep CM (2011) Compact, automatic set-up for ultra-weak photon emission measurements in organisms. In: Microwave & Optoelectronics Conference (IMOC), 2011 SBMO/IEEE MTT-S International 449–452. doi:10.1109/IMOC.2011.6169247
Bertogna E, Bezerra J, Conforti E, Gallep CM (2013) Acute stress in seedlings detected by ultra-weak photon emission. J Photochem Photobiol B 118:74–76. doi:10.1016/j.jphotobiol.2012.11.005
Bevington M (2015) Lunar biological effects and the magnetosphere. Pathophysiology 22:211–222. doi:10.1016/j.pathophys.2015.08.005
Binhi VN, Rubin AB (2007) Magnetobiology: the kT paradox and possible solutions. Electromagn Biol Med 26(1):45–62. doi:10.1080/15368370701205677
Brown FA Jr (1970) Hypothesis of environmental timing of the clock. In: Brown FA Jr, Hastings JW, Palmer JD (eds) The biological clock. Two views. Academic Press, New York, London, pp 13–59
Brown FA Jr, Chow CS (1973) Lunar-correlated variations in water uptake by bean seeds. Biol Bull 145:265–278
Carver BF (ed) (2009) Wheat science and trade. Wiley-Blackwell, Ames, Iowa
Chen W, Xing D, Wang J, He Y (2003) Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition. Luminescence 18:19–24. doi:10.1002/bio.695
Colli L, Facchini U, Guidotti G, Dugnani Lonati R, Orsenigo M, Sommariva O (1955) Further measurements on the bioluminescence of the seedlings. Experientia 11:479–481
Dorda G (2010) Quantisierte Zeit und die Vereinheitlichung von Gravitation und Elektromagnetismus. Göttingen, Cuvillier Verlag
Fisahn J, Yazdanbakhsh N, Klingelé E, Barlow P (2012) Arabidopsis thaliana root growth kinetics and lunisolar tidal acceleration. New Phytol 195:346–355. doi:10.1111/j.1469-8137.2012.04162.x
Fisahn J, Klingelé E, Barlow P (2015) Lunar gravity affects leaf movement of Arabidopsis thaliana in the International Space Station. Planta 241:1509–1518. doi:10.1007/s00425-015-2280-x
Gallep CM (2014) Ultraweak, spontaneous photon emission in seedlings: toxicological and chronobiological applications. Luminescence 29:963–968. doi:10.1002/bio.2658
Gallep CM, Moraes TA, dos Santos SR, Barlow PW (2013) Coincidence of biophoton emission by seedlings during simultaneous, transcontinental germination tests. Protoplasma 250:793–796. doi:10.1007/s00709-012-0447-x
Gallep CM, Moraes TA, Cervinková K, Cifra M, Katsumata M, Barlow PW (2014) Lunisolar tidal synchronism with biophoton emission during intercontinental wheat-seedling germination tests. Plant Signal Behav 9,5:e28671. doi:10.4161/psb.28671
Hoson T, Wakabayashi K (2015) Role of the plant cell wall in gravity resistance. Phytochem 112:84–90. doi:10.1016/j.phytochem.2014.08.022
Kamal AHM, Komatsu S (2015) Involvement of reactive oxygen species and mitochondrial proteins in biophoton emission in roots of soybean plants under flooding tress. J Proteome Res 14:2219–2236. doi:10.1021/acs.jproteome.5b00007
Kanokov Z, Schmelzer J, Nasirov A (2010) New mechanism of solution of the kBT-problem in magnetobiology. Open Physics 8(4):667–671. doi:10.2478/s11534-009-0144-3
Komatsu S, Kamal AHM, Makino T, Hossain Z (2014) Ultraweak photon emission and proteomics analyses in soybean under abiotic stress. Biochim Biophys Acta 1844:1208–1218. doi:10.1016/j.bbapap.2014.04.002
Longman IM (1959) Formulas for computing the tidal acceleration due to the moon and the sun. J Geophys Res 64:2351–2355
Luce GG (1971) Body time. Temple Smith, London
Marchant J (1729) Observation botanique. Histoire de l’Académie Royale des Sciences (Paris), 35
McClung CR, Gutierrez RA (2010) Network news: prime time for systems biology of the plant circadian clock. Curr Opin Genet Dev 20:588–598. doi:10.1016/j.gde.2010.08.010
Miyamoto S, Martinez GR, Medeiros MH, Di Mascio P (2014) Singlet molecular oxygen generated by biological hydroperoxides. J Photochem Photobiol B 139:24–33. doi:10.1016/j.jphotobiol.2014.03.028
Moraes TA, Barlow PW, Klingelé E, Gallep CM (2012) Spontaneous ultra-weak light emissions from wheat seedlings are rhythmic and synchronized with the time profile of the local gravimetric tide. Naturwiss 99:465–472. doi:10.1007/s00114-012-0921-5
Morita MT (2010) Directional gravity sensing in gravitropism. Ann Rev Plant Biol 61:705–720. doi:10.1146/annurev.arplant.043008.092042
Neamţu S, Morariu VV (2005) Plant growth in experimental space flight magnetic field conditions. Roman J Biophys 15:41–46
Palmer JD (2005) The clocks controlling the tide-associated rhythms of intertidal animals. BioEssays 22:32–37
Persinger MA (2014) Terrestrial and lunar gravitational forces upon the mass of a cell: relevance to cell function. Int Lett Chem Phys Astron 2:15–21
Spruyt E, Verbelen J-P, de Greef JA (1987) Expression of circaseptan and circannual rhythmicity in the imbibition of dry stored bean seeds. Plant Physiol 84:707–710
Sweeney BM (1969) Rhythmic phenomena in plants. Academic Press, London
Van Wijk EPA, Bosman S, Van Wijk R (2010) Using ultra-weak photon emission to determine the effect of oligomeric proanthocyanidins on oxidative stress of human skin. J Photochem Photobiol B 98:199–206. doi:10.1016/j.jphotobiol.2010.01.003
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for growth stages of cereals. Weed Res 14:415–421
Zakhvataev VE (2015) Tidal variations of radon activity as a possible factor synchronizing biological processes. Biophysics 60:140–156. doi:10.1134/S0006350915010273
Zhang L, Hastings MH, Green EW, Tauber E, Sladek M, Webster SG, Kyriacou CP, Wilcockson DC (2013) Dissociation of circadian and circatidal timekeeping in the marine crustacean Eurydice pulchra. Curr Biol 23:1863–1873. doi:10.1016/j.cub.2013.08.038
Zürcher E, Cantiani MG, Sorbetti-Guerri F, Michel D (1998) Tree stem diameters fluctuate with tide. Nature 392:665–666. doi:10.1038/33570
Acknowledgments
The authors thank Prof. Emile Klingelé for the gift of the Etide program and also Dr J Fisahn for the valuable information. Thanks are also due to the following funding agencies: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) [no. 14/04232-6, no. 04/10146-3] for partial support to CMG in Brazil and in Leiden/NL and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (CsF no. 230827/2012-8) for the partial support to RCRB in The Netherlands.
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Gallep, C.M., Barlow, P.W., Burgos, R.C.R. et al. Simultaneous and intercontinental tests show synchronism between the local gravimetric tide and the ultra-weak photon emission in seedlings of different plant species. Protoplasma 254, 315–325 (2017). https://doi.org/10.1007/s00709-016-0947-1
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DOI: https://doi.org/10.1007/s00709-016-0947-1