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Identification of cleavage of NS5A of C-strain classical swine fever virus

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Abstract

NS5A is a multifunctional non-structural protein of classical swine fever virus (CSFV) that plays an important role in viral replication, but how it exerts its functions is unknown. Here, we report the cleavage of NS5A of the vaccine C-strain, resulting in two truncated forms (b and c). Further experiments using calpain- and caspase-family-specific inhibitors, followed by a caspase-6-specific shRNAs and inhibitor, showed that the cleavage of C-strain NS5A to produce truncated form c is mediated by caspase-6, mapping to 272DTTD275, while the cleavage producing truncated form b is probably mediated by another unknown protease. shRNA-mediated downregulation of caspase-6 and blocking of enzyme activity in ST cells significantly impaired genome replication and virus production, indicating that NS5A cleavage is required for CSFV replication.

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Acknowledgments

This work was supported by two projects of National Natural Science Foundation of China: Key Project (31130052) to CT and Ordinary Project (31572528) to WG.

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Authors and Affiliations

  1. College of Veterinary Medicine, Jilin University, Changchun, 130062, China

    Jinxin Xie

  2. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, 666 Liuying West Road, Changchun, 130122, China

    Jinxin Xie, Huancheng Guo, Wenjie Gong, Daliang Jiang, Li Zhang, Junjie Jia & Changchun Tu

  3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China

    Changchun Tu

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  1. Jinxin Xie
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  2. Huancheng Guo
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Correspondence to Changchun Tu.

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Xie, J., Guo, H., Gong, W. et al. Identification of cleavage of NS5A of C-strain classical swine fever virus. Arch Virol 162, 391–400 (2017). https://doi.org/10.1007/s00705-016-3117-z

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  • DOI: https://doi.org/10.1007/s00705-016-3117-z

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