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Screening of cellular proteins that interact with the classical swine fever virus non-structural protein 5A by yeast two-hybrid analysis

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Abstract

Classical swine fever virus (CSFV), the pathogen of classical swine fever (CSF), causes severe hemorrhagic fever and vascular necrosis in domestic pigs and wild boar. A large number of evidence has proven that non-structural 5A (NS5A) is not only a very important part of viral replication complex, but also can regulate host cell’s function; however, the underlying mechanisms remain poorly understood. In the current study, aiming to find more clues in understanding the molecular mechanisms of CSFV NS5A’s function, the yeast two-hybrid (Y2H) system was adopted to screen for CSFV NS5A interactive proteins in the cDNA library of the swine umbilical vein endothelial cell (SUVEC). Alignment with the NCBI database revealed 16 interactive proteins: DDX5, PSMC3, NAV1, PHF5A, GNB2L1, CSDE1, HSPA8, BRMS1, PPP2R3C, AIP, TMED10, POLR1C, TMEM70, METAP2, CHORDC1 and COPS6. These proteins are mostly related to gene transcription, protein folding, protein degradation and metabolism. The interactions detected by the Y2H system should be considered as preliminary results. Since identifying novel pathways and host targets, which play essential roles during infection, may provide potential targets for therapeutic development. The finding of proteins obtained from the SUVEC cDNA library that interact with the CSFV NS5A protein provide valuable information for better understanding the interactions between this viral protein and the host target proteins.

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Acknowledgements

The research was supported by China National Science Funds (No. 31172339).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Chengcheng Zhang, Kai Kang, Heng Chen, Lei Xu & Yanming Zhang

  2. College of Animal Science & Technology, Henan University of Science and Technology, Luoyang, 471023, Henan, China

    Lei He

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  1. Chengcheng Zhang
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  2. Lei He
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  3. Kai Kang
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  4. Heng Chen
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Correspondence to Yanming Zhang.

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Corresponding editor: Shahid Jameel

[Zhang C, He L, Kang K, Chen H, Xu L and Zhang Y 2014 Screening of cellular proteins that interact with the classical swine fever virus non-structural protein 5A by yeast two-hybrid analysis. J. Biosci. 39 1–12] DOI 10.1007/s12038-013-9411-y

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Zhang, C., He, L., Kang, K. et al. Screening of cellular proteins that interact with the classical swine fever virus non-structural protein 5A by yeast two-hybrid analysis. J Biosci 39, 63–74 (2014). https://doi.org/10.1007/s12038-013-9411-y

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  • DOI: https://doi.org/10.1007/s12038-013-9411-y

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