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Complete genome sequence of the Pectobacterium carotovorum subsp. carotovorum virulent bacteriophage PM1

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Abstract

PM1, a novel virulent bacteriophage that infects Pectobacterium carotovorum subsp. carotovorum, was isolated. Its morphological features were examined by electron microscopy, which indicated that this phage belongs to the family Myoviridae. It has a 55,098-bp genome, including a 2,665-bp terminal repeat. A total of 63 open reading frames (ORFs) were predicted, but only 20 ORFs possessed homology with functional proteins. There is one tRNA coding region, and the GC-content of the genome is 44.9 %. Most ORFs in bacteriophage PM1 showed high homology to enterobacteria phage ΦEcoM-GJ1 and Erwinia phage νB EamM-Y2. Like these bacteriophages, PM1 encodes an RNA polymerase, which is a hallmark of T7-like phages. There is no integrase or repressor, suggesting that PM1 is a virulent bacteriophage.

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Acknowledgments

This work was supported by the Rural Development Administration (RDA) fund (PJ008639) and Post-Doc Fellowship to JL and DL from RDA.

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Authors and Affiliations

  1. Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea

    Jeong-A Lim, Dong Hwan Lee & Sunggi Heu

  2. Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Seoul National University, Seoul, 151-921, Korea

    Hakdong Shin & Sangryeol Ryu

  3. Department of Integrative Plant Science, Chung-Ang University, Anseong, 456-756, Korea

    Sang-Wook Han

  4. Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea

    Ju-Hoon Lee

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  1. Jeong-A Lim
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  2. Hakdong Shin
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  3. Dong Hwan Lee
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  5. Ju-Hoon Lee
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  7. Sunggi Heu
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Corresponding author

Correspondence to Sunggi Heu.

Additional information

The nucleotide sequence data reported are available in the GenBank database under the accession number KF534715.

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Lim, JA., Shin, H., Lee, D. et al. Complete genome sequence of the Pectobacterium carotovorum subsp. carotovorum virulent bacteriophage PM1. Arch Virol 159, 2185–2187 (2014). https://doi.org/10.1007/s00705-014-2005-7

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  • DOI: https://doi.org/10.1007/s00705-014-2005-7

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