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Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) genotypes I and II in Thailand

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Abstract

To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Thailand, 279 ORF5 sequences of PRRSV collected during 2010–2011 from 102 swine herds in five swine-producing areas were analyzed. The co-existence of European (EU) and North American (NA) genotypes was observed in 98 % of herds investigated and was evident at the pig level. Both genotypes have evolved separately with a temporal influence on strain development. Novel introductions influence the genetic diversity of the NA genotype. Although Thai EU and NA isolates develop their own clusters that are separate from those of other countries, there was no geographic influence on strain development within Thailand.

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References

  1. Damrongwatanapokin S, Arsayuth K, Kongkrong C, Pachariyanonn S, Pinyochon W, Tantaswasdi U (1996) Serological studies and isolation of porcine reproductive and respiratory syndrome (PRRS) virus in Thailand. J Thai Vet Med Assoc 47:19–30

    Google Scholar 

  2. Feng Y, Zhao T, Nguyen T, Inui K, Ma Y, Nguyen TH, Nguyen VC, Liu D, Bui QA, To LT, Wang C, Tian K, Gao GF (2008) Porcine respiratory and reproductive syndrome virus variants, Vietnam and China, 2007. Emerg Infect Dis 14:1774–1776

    Article  PubMed  CAS  Google Scholar 

  3. Forsberg R, Storgaard T, Nielsen HS, Oleksiewicz MB, Cordioli P, Sala G, Hein J, Botner A (2002) The genetic diversity of European type PRRSV is similar to that of the North American type but is geographically skewed within Europe. Virology 299:38–47

    Article  PubMed  CAS  Google Scholar 

  4. Forsberg R (2005) Divergence time of porcine reproductive and respiratory syndrome virus subtypes. Mol Biol Evol 22:2131–2134

    Article  PubMed  CAS  Google Scholar 

  5. Goldberg TL, Lowe JF, Milburn SM, Firkins LD (2003) Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection. Virology 317:197–207

    Article  PubMed  CAS  Google Scholar 

  6. Gonin P, Pirzadeh B, Gagnon CA, Dea S (1999) Seroneutralization of porcine reproductive and respiratory syndrome virus correlates with antibody response to the GP5 major envelope glycoprotein. J Vet Diagn Invest 11:20–26

    Article  PubMed  CAS  Google Scholar 

  7. Greiser-Wilke I, Fiebig K, Drexler C, grosse Beilage E (2010) Genetic diversity of Porcine reproductive and respiratory syndrome virus (PRRSV) in selected herds in a pig-dense region of North-Western Germany. Vet Microbiol 143:213–223

    Google Scholar 

  8. Indik S, Valicek L, Klein D, Klanova J (2000) Variations in the major envelope glycoprotein GP5 of Czech strains of porcine reproductive and respiratory syndrome virus. J Gen Virol 81:2497–2502

    PubMed  CAS  Google Scholar 

  9. Indik S, Schmoll F, Sipos W, Klein D (2005) Genetic variability of PRRS virus in Austria: consequences for molecular diagnostics and viral quantification. Vet Microbiol 107:171–178

    Article  PubMed  CAS  Google Scholar 

  10. Johnson CR, Griggs TF, Gnanandarajah J, Murtaugh MP (2011) Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses. J Gen Virol 92:1107–1116

    Article  PubMed  CAS  Google Scholar 

  11. Kim SH, Roh IS, Choi EJ, Lee C, Lee CH, Lee KH, Lee KK, Song YK, Lee OS, Park CK (2010) A molecular analysis of European porcine reproductive and respiratory syndrome virus isolated in South Korea. Vet Microbiol 143:394–400

    Article  PubMed  CAS  Google Scholar 

  12. Lager KM, Mengeling WL, Brockmeier SL (1997) Homologous challenge of porcine reproductive and respiratory syndrome virus immunity in pregnant swine. Vet Microbiol 58:113–125

    Article  PubMed  CAS  Google Scholar 

  13. Lee C, Kim H, Kang B, Yeom M, Han S, Moon H, Park S, Song D, Park B (2010) Prevalence and phylogenetic analysis of the isolated type I porcine reproductive and respiratory syndrome virus from 2007 to 2008 in Korea. Virus Genes 40:225–230

    Article  PubMed  CAS  Google Scholar 

  14. Mengeling WL, Lager KM, Vorwald AC, Clouser DF (2003) Comparative safety and efficacy of attenuated single-strain and multi-strain vaccines for porcine reproductive and respiratory syndrome. Vet Microbiol 93:25–38

    Article  PubMed  CAS  Google Scholar 

  15. Meulenberg JJ, Petersen den Besten A, de Kluyver E, van Nieuwstadt A, Wensvoort G, Moormann RJ (1997) Molecular characterization of Lelystad virus. Vet Microbiol 55:197–202

    Article  PubMed  CAS  Google Scholar 

  16. Murtaugh MP, Faaberg KS, Laber J, Elam M, Kapur V (1998) Genetic variation in the PRRS virus. Adv Exp Med Biol 440:787–794

    Article  PubMed  CAS  Google Scholar 

  17. Nei M, Gojobori T (1986) Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol 3:418–426

    PubMed  CAS  Google Scholar 

  18. Nelsen CJ, Murtaugh MP, Faaberg KS (1999) Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents. J Virol 73:270–280

    PubMed  CAS  Google Scholar 

  19. Nilubol D, Platt KB, Halbur PG, Torremorell M, Harris DL (2004) The effect of a killed porcine reproductive and respiratory syndrome virus (PRRSV) vaccine treatment on virus shedding in previously PRRSV infected pigs. Vet Microbiol 102:11–18

    Article  PubMed  CAS  Google Scholar 

  20. Ostrowski M, Galeota JA, Jar AM, Platt KB, Osorio FA, Lopez OJ (2002) Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol 76:4241–4250

    Article  PubMed  CAS  Google Scholar 

  21. Pesch S, Meyer C, Ohlinger VF (2005) New insights into the genetic diversity of European porcine reproductive and respiratory syndrome virus (PRRSV). Vet Microbiol 107:31–48

    Article  PubMed  CAS  Google Scholar 

  22. Plagemann PG, Rowland RR, Faaberg KS (2002) The primary neutralization epitope of porcine respiratory and reproductive syndrome virus strain VR-2332 is located in the middle of the GP5 ectodomain. Arch Virol 147:2327–2347

    Article  PubMed  CAS  Google Scholar 

  23. Plagemann PG (2004) GP5 ectodomain epitope of porcine reproductive and respiratory syndrome virus, strain Lelystad virus. Virus Res 102:225–230

    Article  PubMed  CAS  Google Scholar 

  24. Simonsen M, Mailund T, Pedersen CNS (2008) Rapid Neighbour Joining. In: In proceeding of the 8th Workshop in Algorithms in Bioinformatics (WABI). Springer Verlag, pp 113-122

  25. Stadejek T, Stankevicius A, Storgaard T, Oleksiewicz MB, Belak S, Drew TW, Pejsak Z (2002) Identification of radically different variants of porcine reproductive and respiratory syndrome virus in Eastern Europe: towards a common ancestor for European and American viruses. J Gen Virol 83:1861–1873

    PubMed  CAS  Google Scholar 

  26. Stadejek T, Oleksiewicz MB, Potapchuk D, Podgorska K (2006) Porcine reproductive and respiratory syndrome virus strains of exceptional diversity in eastern Europe support the definition of new genetic subtypes. J Gen Virol 87:1835–1841

    Article  PubMed  CAS  Google Scholar 

  27. Stadejek T, Oleksiewicz MB, Scherbakov AV, Timina AM, Krabbe JS, Chabros K, Potapchuk D (2008) Definition of subtypes in the European genotype of porcine reproductive and respiratory syndrome virus: nucleocapsid characteristics and geographical distribution in Europe. Arch Virol 153:1479–1488

    Article  PubMed  CAS  Google Scholar 

  28. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  29. Thanawongnuwech R, Amonsin A, Tatsanakit A, Damrongwatanapokin S (2004) Genetics and geographical variation of porcine reproductive and respiratory syndrome virus (PRRSV) in Thailand. Vet Microbiol 101:9–21

    Article  PubMed  CAS  Google Scholar 

  30. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  31. Tun HM, Shi M, Wong CL, Ayudhya SN, Amonsin A, Thanawonguwech R, Leung FC (2011) Genetic diversity and multiple introductions of porcine reproductive and respiratory syndrome viruses in Thailand. Virol J 8:164

    Article  PubMed  Google Scholar 

  32. Weiland E, Wieczorek-Krohmer M, Kohl D, Conzelmann KK, Weiland F (1999) Monoclonal antibodies to the GP5 of porcine reproductive and respiratory syndrome virus are more effective in virus neutralization than monoclonal antibodies to the GP4. Vet Microbiol 66:171–186

    Article  PubMed  CAS  Google Scholar 

  33. Wissink EH, van Wijk HA, Kroese MV, Weiland E, Meulenberg JJ, Rottier PJ, van Rijn PA (2003) The major envelope protein, GP5, of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain. J Gen Virol 84:1535–1543

    Article  PubMed  CAS  Google Scholar 

  34. Wu WH, Fang Y, Farwell R, Steffen-Bien M, Rowland RR, Christopher-Hennings J, Nelson EA (2001) A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Virology 287:183–191

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank Dr. Erin Strait for reviewing the manuscript. The authors are grateful to the Thailand Research Fund (project number MRG5080323 and IUG5080001) and the Government budget year 2011–2012 for funding this research.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Dachrit Nilubol, Thitima Tripipat & Tawatchai Hoonsuwan

  2. Genome Institute, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand

    Pavita Tipsombatboon & Jittima Piriyapongsa

Authors
  1. Dachrit Nilubol
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  2. Thitima Tripipat
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  3. Tawatchai Hoonsuwan
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  4. Pavita Tipsombatboon
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  5. Jittima Piriyapongsa
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Corresponding author

Correspondence to Dachrit Nilubol.

Electronic supplementary material

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Supplementary material 1 (JPEG 160 kb)

Supplementary material 2 (DOCX 12 kb)

705_2012_1573_MOESM3_ESM.pdf

Supplementary material 3 Comparison of the amino acid substitutions that are found in glycoprotein 5 of the European genotype and reference isolates. The functional domains, which include the decoy and primary neutralizing epitopes, are indicated in boxes

705_2012_1573_MOESM4_ESM.pdf

Supplementary material 4 Comparison of the amino acid substitutions that are found in glycoprotein 5 of the North American genotype and reference isolates. The functional domains, which include the decoy and primary neutralizing epitopes, are indicated in boxes

Supplementary material 5 (JPEG 334 kb)

Supplementary material 6 (JPEG 192 kb)

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Nilubol, D., Tripipat, T., Hoonsuwan, T. et al. Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) genotypes I and II in Thailand. Arch Virol 158, 943–953 (2013). https://doi.org/10.1007/s00705-012-1573-7

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  • DOI: https://doi.org/10.1007/s00705-012-1573-7

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