Skip to main content
SpringerLink
Log in

Prevalence and phylogenetic analysis of the isolated type I porcine reproductive and respiratory syndrome virus from 2007 to 2008 in Korea

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

The first Korean strain of porcine reproductive and respiratory syndrome virus (PRRSV) was isolated in 1997, and it exhibited high similarity to strain VR-2332 (type II PRRSV; North American type). Recently, however, infection with type I PRRSV (European type) has also been reported in Korea. To date, preliminary data about type I PRRSV prevalence in Korea have not been reported. Here, using reverse transcriptase (RT)-PCR, we analyzed 383 archived field samples from 101 pig farms in Korea that were collected from 2007 to 2008. We identified 155 samples from 68 farms that were positive for PRRSV. Fifty-one samples (51/155; 32.9%) and 20 farms (20/68; 29.4%) were type I PRRSV-positive/type II PRRSV-negative. Furthermore, we tried to isolate the type I PRRSV from positive samples and seven type I PRRSV were isolated using PAM. The phylogenetic analysis using the type I PRRSV isolates (7 isolates) was performed based on open reading frame (ORF)5 (accession numbers GU325642 to GU325648) and ORF7 (accession numbers GU325635 to GU325641). In the phylogenetic study, seven type I PRRSV isolates were closely related with panEuropean based on ORF7, while they were genetically distinct from Lelystad virus and made a unique clade based on ORF5. The results of this study demonstrate that infection with type I PRRSV is not uncommon in Korean pig farms, which suggests that diagnosis and control of type I PRRSV should be considered in Korea. A new approach to vaccination against, and epidemiological analysis of, Korean PRRSV is urgently needed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. V.G. Andreyev, R.D. Wesley, W.L. Mengeling, A.C. Vorwald, K.M. Lager, Arch. Virol. 142, 993–1001 (1997)

    Article  CAS  PubMed  Google Scholar 

  2. E.M. Bautista, S.M. Goyal, J.E. Collins, J. Vet. Diagn. Invest. 5, 612–614 (1993)

    CAS  PubMed  Google Scholar 

  3. D.A. Benfield, E. Nelson, J.E. Collins, L. Harris, S.M. Goyal, D. Robison, W.T. Christianson, R.B. Morrison, D. Gorcyca, D. Chladek, J. Vet. Diagn. Invest. 4, 127–133 (1992)

    CAS  PubMed  Google Scholar 

  4. D. Cavanagh, Arch. Virol. 142, 629–633 (1997)

    CAS  PubMed  Google Scholar 

  5. S.H. Cha, E.J. Choi, J.H. Park, S.R. Yoon, J.Y. Song, J.H. Kwon, H.J. Song, K.J. Yoon, Vet. Microbiol. 117, 248–257 (2006)

    Article  CAS  PubMed  Google Scholar 

  6. J.E. Collins, D.A. Benfield, W.T. Christianson, L. Harris, J.C. Hennings, D.P. Shaw, S.M. Goyal, S. McCullough, R.B. Morrison, H.S. Joo, D. Gorcyca, D. Chladek, J. Vet. Diagn. Invest. 4, 117–126 (1992)

    CAS  PubMed  Google Scholar 

  7. C.A. Gagnon, S. Dea, Can. J. Vet. Res. 62, 110–116 (1998)

    CAS  PubMed  Google Scholar 

  8. T.L. Goldberg, E.C. Hahn, R.M. Weigel, G. Scherba, J. Gen. Virol. 81, 171–179 (2000)

    CAS  PubMed  Google Scholar 

  9. P.G. Halbur, P.S. Paul, M.L. Frey, J. Landgraf, K. Eernisse, X.J. Meng, J.J. Andrews, M.A. Lum, J.A. Rathje, Vet. Pathol. 33, 159–170 (1996)

    Article  CAS  PubMed  Google Scholar 

  10. S. Indik, L. Valicek, D. Klein, J. Klanova, J. Gen. Virol. 81, 2497–2502 (2000)

    CAS  PubMed  Google Scholar 

  11. S.Y. Kang, S.I. Yun, H.S. Park, C.K. Park, H.S. Choi, Y.M. Lee, Virus Res. 104, 165–179 (2004)

    Article  CAS  PubMed  Google Scholar 

  12. V. Kapur, M.R. Elam, T.M. Pawlovich, M.P. Murtaugh, J. Gen. Virol. 77, 1271–1276 (1996)

    Article  CAS  PubMed  Google Scholar 

  13. H.K. Kim, J.S. Yang, H.J. Moon, S.J. Park, Y. Luo, C.S. Lee, D.S. Song, B.K. Kang, S.K. Ann, C.H. Jun, B.K. Park, J. Vet. Sci. 10, 121–130 (2009)

    Article  PubMed  Google Scholar 

  14. J. Kwang, H.S. Kim, H.S. Joo, J. Vet. Diagn. Invest. 6, 293–296 (1994)

    CAS  PubMed  Google Scholar 

  15. K.G. Madsen, C.M. Hansen, E.S. Madsen, B. Strandbygaard, A. Botner, K.J. Sorensen, Arch. Virol. 143, 1683–1700 (1998)

    Article  CAS  PubMed  Google Scholar 

  16. H. Mardassi, S. Mounir, S. Dea, J. Gen. Virol. 75, 681–685 (1994)

    Article  CAS  PubMed  Google Scholar 

  17. X.J. Meng, Vet. Microbiol. 74, 309–329 (2000)

    Article  CAS  PubMed  Google Scholar 

  18. X.J. Meng, P.S. Paul, P.G. Halbur, J. Gen. Virol. 75, 1795–1801 (1994)

    Article  CAS  PubMed  Google Scholar 

  19. X.J. Meng, P.S. Paul, P.G. Halbur, M.A. Lum, Arch. Virol. 140, 745–755 (1995)

    Article  CAS  PubMed  Google Scholar 

  20. X.J. Meng, P.S. Paul, P.G. Halbur, I. Morozov, J. Gen. Virol. 76, 3181–3188 (1995)

    Article  CAS  PubMed  Google Scholar 

  21. I. Morozov, X.J. Meng, P.S. Paul, Arch. Virol. 140, 1313–1319 (1995)

    Article  CAS  PubMed  Google Scholar 

  22. M.P. Murtaugh, M.R. Elam, L.T. Kakach, Arch. Virol. 140, 1451–1460 (1995)

    Article  CAS  PubMed  Google Scholar 

  23. C.J. Nelsen, M.P. Murtaugh, K.S. Faaberg, J. Virol. 73, 270–280 (1999)

    CAS  PubMed  Google Scholar 

  24. B. Pirzadeh, C.A. Gagnon, S. Dea, Can. J. Vet. Res. 62, 170–177 (1998)

    CAS  PubMed  Google Scholar 

  25. M.B. Oleksiewicz, A. Botner, K.G. Madsen, T. Storgaard, Vet. Microbiol. 64, 7–22 (1998)

    Article  CAS  PubMed  Google Scholar 

  26. C. Prieto, A. Vazquez, J.I. Nunez, E. Alvarez, I. Simarro, J.M. Castro, Vet. J. 180, 363–370 (2009)

    Article  CAS  PubMed  Google Scholar 

  27. T. Stadejek, M.B. Oleksiewicz, D. Potapchuk, K. Podgorska, J. Gen. Virol. 87, 1835–1841 (2006)

    Article  CAS  PubMed  Google Scholar 

  28. T. Stadejek, M.B. Oleksiewicz, A.V. Scherbakov, A.M. Timina, J.S. Krabbe, K. Chabros, D. Potapchuk, Arch. Virol. 153, 1479–1488 (2008)

    Article  CAS  PubMed  Google Scholar 

  29. P. Suarez, R. Zardoya, M.J. Martin, C. Prieto, J. Dopazo, A. Solana, J.M. Castro, Virus Res. 42, 159–165 (1996)

    Article  CAS  PubMed  Google Scholar 

  30. P.A. van Woensel, K. Liefkens, S. Demaret, Vet. Rec. 142, 510–512 (1998)

    PubMed  Google Scholar 

  31. G. Wensvoort, C. Terpstra, J.M.A. Pol, E.A. Terlaak, M. Bloemraad, E.P. Dekluyver, C. Kragten, L. Vanbuiten, A. Denbesten, F. Wagenaar, J.M. Broekhuijsen, P. Moonen, T. Zetstra, E.A. Deboer, H.J. Tibben, M.F. Dejong, P. Vantveld, G.J.R. Groenland, J.A. Vangennep, M.T. Voets, J.H.M. Verheijden, J. Braamskamp, Vet. Q. 13, 121–130 (1991)

    CAS  PubMed  Google Scholar 

  32. E.H.J. Wissink, H.A.R. van Wijk, M.V. Kroese, E. Weiland, J.J.M. Meulenberg, P.J.M. Rottier, P.A. van Rijn, J. Gen. Virol. 84, 1535–1543 (2003)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

Author information

Authors and Affiliations

  1. Chung-Aang University School of Medicine, Seoul, 156-756, Republic of Korea

    Chulseung Lee

  2. Department of Veterinary Medicine, Virology Laboratory, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, 151-742, Republic of Korea

    Hyekwon Kim & Bongkyun Park

  3. Research Unit, Green Cross Veterinary Products, 227-5, Kugal-dong, Kiheung-gu, Yongin, 449-903, Republic of Korea

    Bokyu Kang, Minjoo Yeom, Sangyoon Han, Hyoungjoon Moon, Seongjun Park & Daesub Song

  4. Optifarm Solution, Inc., 48 Songnam-Ri Seonggo-UB, Chunan City, Chungnam Province, Republic of Korea

    Hyunil Kim

Authors
  1. Chulseung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyekwon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bokyu Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Minjoo Yeom
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sangyoon Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hyoungjoon Moon
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Seongjun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hyunil Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Daesub Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Bongkyun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Daesub Song or Bongkyun Park.

Additional information

Hyekwon Kim and Bokyu Kang contributed equally to this work.

Chulseung Lee, Hyekwon Kim, and Bokyu Kang are co-first authors.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, C., Kim, H., Kang, B. et al. Prevalence and phylogenetic analysis of the isolated type I porcine reproductive and respiratory syndrome virus from 2007 to 2008 in Korea. Virus Genes 40, 225–230 (2010). https://doi.org/10.1007/s11262-009-0433-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-009-0433-3

Keywords

Search

Navigation