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Complete sequence of a cryptic virus from hemp (Cannabis sativa)

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Abstract

Hemp (Cannabis sativa) was found to be a useful propagation host for hop latent virus, a carlavirus. However, when virus preparations were analysed by electron microscopy, along with the expected filamentous particles, spherical particles with a diameter of around 34 nm were found. RNA from virus preparations was purified, and cDNA was prepared and cloned. Sequence information was used to search databases, and the greatest similarity was found with Primula malacoides virus 1, a putative new member of the genus Partitivirus. The full sequences of RNA 1 and RNA 2 of this new hemp cryptic virus were obtained.

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Acknowledgments

Hemp varieties were a gift from A. Dornbusch, KWS Einbeck. The work was carried out under license 4581695 from Bundesinstitut für Arzneimittel und Medizinprodukte, Bundesopiumstelle. We thank Dr. Jutta Gabler (JKI Quedlinburg) for the mycological analysis of the plant material, and Elke Zimmermann (JKI Quedlinburg) for electron microscopy. The authors acknowledge funding by the Czech Science Foundation (GACR P501/10/J018) to J.M. and by the German Research Foundation to J.S. and G.S.

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Authors and Affiliations

  1. Institute for Biosafety of Genetically Modified Plants, Julius Kühn-Institute, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Angelika Ziegler & Jörg Schubert

  2. Department of Biophysics, Heinrich-Heine-University, Build. 26.12, U1, Universitätsstr. 1, 40225, Düsseldorf, Germany

    Gerhard Steger

  3. Biol. Centre AS CR v.v.i., Institute of Plant Molecular Biology, 37005, České Budějovice, Czech Republic

    Jaroslav Matoušek

Authors
  1. Angelika Ziegler
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  2. Jaroslav Matoušek
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  3. Gerhard Steger
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  4. Jörg Schubert
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Corresponding author

Correspondence to Angelika Ziegler.

Additional information

The sequences reported in this paper have been submitted to the GenBank database and have been assigned the accession numbers JN196536 and JN196537.

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Ziegler, A., Matoušek, J., Steger, G. et al. Complete sequence of a cryptic virus from hemp (Cannabis sativa). Arch Virol 157, 383–385 (2012). https://doi.org/10.1007/s00705-011-1168-8

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  • DOI: https://doi.org/10.1007/s00705-011-1168-8

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