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Caspase- and p38-MAPK-dependent induction of apoptosis in A549 lung cancer cells by Newcastle disease virus

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Abstract

Newcastle disease virus (NDV) has a potential oncolytic effect due to its ability to induce apoptosis in tumor cells. However, previous studies have indicated discrepancies regarding the apoptosis signaling pathways induced by NDV in tumor cells. Here, we show that NDV infection induces simultaneous activation of intrinsic and extrinsic death pathways in A549 human lung cancer cells. In contrast, endoplasmic reticulum (ER) stress is not activated in NDV-induced apoptosis. We demonstrate for the first time that mitogen-activated protein kinase (MAPK) pathways are activated in NDV-infected A549 cells, and p38 MAPK is involved in NDV-induced cell death. Together, our findings provide novel insights into the underlying mechanisms by which NDV induces apoptosis in tumor cells.

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Acknowledgments

We are grateful to Prof. Bu Zhigao and Dr. Jia Lijun for helpful discussions. This work was supported by grants from National Key Laboratory of Veterinary Biotechnology of China (SKLVBF200903), Earmarked Fund for Modern Agro-industry Technology Research System, Department of Science and Technology of Zhejiang Province (2009R100310) and Natural Science Foundation of Zhejiang Province (Y2080323).

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Authors and Affiliations

  1. National Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Harbin, 150001, Heilongjiang, China

    Jianchun Bian, Xiangang Kong, Huairan Liu & Songshu Meng

  2. School of Veterinary Medicine, Yangzhou University, 48 East, Wenhui Road, Yangzhou, 225009, Jiangsu, China

    Jianchun Bian, Fei Chen, Maozhi Hu, Xiaorong Zhang, Xinan Jiao, Yantao Wu & Songshu Meng

  3. Department of Respiratory Medicine, The Second Affiliated Hospital of Zhejiang University, Hangzhou, 310058, Zhejiang, China

    Kai Wang

  4. Institute for Health Sciences, Chinese Academy of Sciences, Shanghai, 200025, China

    Baoxue Ge

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  1. Jianchun Bian
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  2. Kai Wang
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Correspondence to Yantao Wu or Songshu Meng.

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J. Bian and K. Wang contributed equally to this work.

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Bian, J., Wang, K., Kong, X. et al. Caspase- and p38-MAPK-dependent induction of apoptosis in A549 lung cancer cells by Newcastle disease virus. Arch Virol 156, 1335–1344 (2011). https://doi.org/10.1007/s00705-011-0987-y

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  • DOI: https://doi.org/10.1007/s00705-011-0987-y

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