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Cerebral amyloid angiopathy in streptozotocin rat model of sporadic Alzheimer’s disease: a long-term follow up study

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Abstract

Cerebral amyloid angiopathy is manifested as accumulation of amyloid β (Aβ) peptide in the wall of meningeal and cerebral arteries, arterioles and capillaries and is frequently found postmortem in sporadic Alzheimer’s disease (sAD) patients. It is difficult to assess when and how cerebral amyloid angiopathy develops and progresses in humans in vivo, which is why animal AD models are used. Streptozotocin-intracerebroventricularly (STZ-icv) treated rats have been recently proposed as the model of sAD which develops insulin resistant brain state preceding Aβ pathology development. Vascular Aβ deposits in the brain of STZ-icv-treated rats (3 months old at the time of icv treatment) were visualized by Thioflavine-S staining, Congo red staining and Aβ immunohistochemistry. Thioflavine-S and Congo red staining revealed diffuse congophilic deposits in the wall of meningeal and cortical blood vessels both 6 and 9 months after the STZ-icv treatment. Preliminary Aβ1-42 and Aβ1-16 immunohistochemistry experiments showed positive staining in blood vessels 3 and 9 months after the STZ-icv treatment, respectively. Results suggest that cerebral amyloid angiopathy observed 6 and 9 months after the STZ-icv treatment seems to be a continuation and progression of the amyloid pathology observed already 3 months following the STZ-icv treatment in this non-transgenic sAD animal model.

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Acknowledgments

Supported by Deutscher Akademischer Austausch Dienst (project No. A/04/20017), and the Croatian Ministry of Science, Education and Sport (project No. 0108253). Special thanks to Mrs Bozica Hrzan for technical assistance.

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Authors and Affiliations

  1. Department of Pharmacology and Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Salata 11, HR 10 000, Zagreb, Croatia

    Melita Salkovic-Petrisic & Jelena Osmanovic-Barilar

  2. Department of Molecular and Cellular Mechanisms of Neurodegeneration, Paul Flechsig Institute for Brain Research, University Leipzig, Jahnallee 59, 04109, Leipzig, Germany

    Martina K. Brückner & Thomas Arendt

  3. Department of Pathology, University Clinic, University of Heidelberg, Im Neuenheimer Feld 220/221, 69120, Heidelberg, Germany

    Siegfried Hoyer

  4. Clinic and Policlinic of Psychiatry, Psychosomatic and Psychotherapy Department, Clinical Neurochemistry, University of Wuerzburg, Fuechsleinstr. 15, 97080, Wuerzburg, Germany

    Peter Riederer

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  1. Melita Salkovic-Petrisic
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  3. Martina K. Brückner
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  4. Siegfried Hoyer
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Correspondence to Melita Salkovic-Petrisic.

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Salkovic-Petrisic, M., Osmanovic-Barilar, J., Brückner, M.K. et al. Cerebral amyloid angiopathy in streptozotocin rat model of sporadic Alzheimer’s disease: a long-term follow up study. J Neural Transm 118, 765–772 (2011). https://doi.org/10.1007/s00702-011-0651-4

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  • DOI: https://doi.org/10.1007/s00702-011-0651-4

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