Abstract
Based on three chloroplast markers (psbA-trnH, trnL-trnF, and trnT-trnL), we identified eight haplotypes in 23 samples of Litsea coreana collected from seven provinces across southern and southwestern China. The Hap6 was the most widely distributed haplotype. The genetic diversity was highest in Sichuan Province and its adjacent regions. Therefore, these regions should be areas of priority for conservation of L. coreana in the future. In addition, our molecular dating analyses indicated that the uplift of the Tibetan Plateau and past climate changes accounted for the diversification of L. coreana in China. Finally, we found that the chemical components of L. coreana were significantly affected by both collection time and postharvest handling methods.
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Acknowledgments
This research was supported by the National Natural Science Foundation of P. R. China (No. 81072995, No. 81274188). The authors are very grateful to Dr. Jin-feng Chen (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for his valuable scripts to phylogeny construction.
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F. Han and L. Xu contributed equally to this work.
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606_2014_1031_MOESM1_ESM.doc
Supplementary material 1 (DOC 91 kb) Supplementary Doc 1 BI tree based on three chloroplast markers psbA-trnH, trnL-trnF, and trnT-trnL, with eight sequences from Monimiaceae, Hernandiaceae, and Gomortegaceae as outgroups
606_2014_1031_MOESM2_ESM.pdf
Supplementary material 2 (PDF 11 kb) GenBank accession numbers for the psbA-trnH, trnL-trnF, and trnT-trnL markers from Lauraceae
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Han, F., Xu, L., Peng, Y. et al. The pattern of genetic diversity within Litsea coreana (Lauraceae) in China: an implication for conservation. Plant Syst Evol 300, 2229–2238 (2014). https://doi.org/10.1007/s00606-014-1031-y
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DOI: https://doi.org/10.1007/s00606-014-1031-y