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Genetic relationship among wild, landraces and cultivars of hazelnut (Corylus avellana) from Portugal revealed through ISSR and AFLP markers

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Abstract

The genus Corylus, a member of the birch family Betulaceae, includes several species that are widely distributed throughout temperate regions of the Northern Hemisphere. This study assesses the genetic diversity in 26 international cultivars and 32 accessions of Corylus avellana L. from Portugal: 13 wild genotypes and 19 landraces. The genetic relationships among the 58 hazelnuts (Corylus avellana L.) were analyzed using inter simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) markers. Eighteen ISSR primers and seven AFLP primer pairs generated a total of 570 unambiguous and repeatable bands, respectively, from which 541 (95.03 %) were polymorphic for both markers. Genetic similarity index values ranged from 0.239 for wild types and cultivars to 0.143 for landraces and wild types. The genetic relationships were presented as a Neighbor-Joining method dendrogram and a two-dimensional principal coordinate analysis (PCoA) plot. The Neighbor-Joining dendrogram showed three main clusters, and the PCoA analysis has shown to be congruent with the hierarchical analysis. Bayesian analysis clustered all individuals into three groups showing a good separation among wild genotypes, landraces and cultivars. The genetic diversity found on wild genotypes and Portuguese landraces may provide relevant information for the diversity conservation and it will be useful in breeding programs and to identify local selections for preservation.

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Acknowledgments

This work was supported by the PhD grant SFRH/BD/40686/2007 from the Portuguese Foundation for Science and Technology (FCT).

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Authors and Affiliations

  1. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801, Vila Real, Portugal

    Sandra Martins & Valdemar Carnide

  2. Biotechnology and Genetic Resources Research Unit, National Institute for Agrarian and Veterinarian Research I.P., Avenida da República, Quinta do Marquês, 2784-505, Oeiras, Portugal

    Fernanda Simões & José Matos

  3. Centre for the Research and Technology of Agro-Environment and Biological Sciences, University of Trás-os-Montes and Alto Douro, 5001-801, Vila Real, Portugal

    Ana Paula Silva

  4. Centre of Genomics and Biotechnology, Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, 5001-801, Vila Real, Portugal

    Valdemar Carnide

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  1. Sandra Martins
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  4. Ana Paula Silva
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Correspondence to Sandra Martins.

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Martins, S., Simões, F., Matos, J. et al. Genetic relationship among wild, landraces and cultivars of hazelnut (Corylus avellana) from Portugal revealed through ISSR and AFLP markers. Plant Syst Evol 300, 1035–1046 (2014). https://doi.org/10.1007/s00606-013-0942-3

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