Skip to main content
SpringerLink
Log in

Molecular cytogenetic analysis of Siberian Larix species by fluorescence in situ hybridization

  • Short Communication
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Karyotypes of three Larix species (L. sibirica, L. gmelinii, and L. cajanderi) were analyzed using fluorescence in situ hybridization (FISH) with 45S and 5S ribosomal RNA gene probes and 4′,6-diamidino-2-phenylindole (DAPI) staining. Two major 45S ribosomal DNA (rDNA) loci (per haploid genome) have been observed in the intercalary regions of two metacentric chromosomes, III and IV, of L. sibirica; in addition to them, minor nucleolus organizing regions (NORs) were mapped in pericentromeric regions of chromosomes I, II, VI, and XII. Two closely related species, L. gmelinii and L. cajanderi, showed similar hybridization patterns; both species possessed an additional major locus of 45S rDNA in the distal region of the long arm of submetacentric chromosome VII that is absent in L. sibirica. Only one locus of the 5S rDNA was found in all larch species we studied; it was located in the distal region of the chromosome III short arm, which also carried the major NOR in the opposite arm. This chromosome containing major loci of the two ribosomal RNA gene families can serve as a marker of the genus Larix. The intra- and interspecific karyotype diversity in the genus Larix is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Abbreviations

FISH:

Fluorescence in situ hybridization

DAPI:

4′,6-Diamidino-2-phenylindole

SSC:

Saline-sodium citrate

PBS:

Phosphate-buffered saline

NOR:

Nucleolus organizing region

rDNA:

Ribosomal DNA

Mbp:

Million base pairs

References

  • Abaimov AP (2010) Geographical distribution and genetics of Siberian larch species. In: Osawa A, Zyryanova OA, Matsuura Y, Kajimoto T, Wein RW (eds.) Permafrost Ecosystems: Siberian Larch Forests. Ser.: Ecological Studies, 209, pp. 41–58

  • Abaimov AP, Koropachinsky IYu (1984) Gmelin and Cajander larches. Novosibirsk, Nauka, p 120

  • Bobrov EG (1972) History and systematics of Larix. Nauka, Leningrad (in Russian)

    Google Scholar 

  • Bogunic F, Siljak-Yakovlev S, Muratovic E, Ballian D (2011) Different karyotype patterns among allopatric Pinus nigra (Pinaceae) populations revealed by molecular cytogenetics. Plant Biol 13:194–200

    Article  PubMed  CAS  Google Scholar 

  • Cai Q, Zhang DM, Liu ZL, Wang XR (2006) Chromosomal localization of 5S and 18S rDNA in five species of subgenus Strobus and their implications for genome evolution of Pinus. Ann Bot 97(5):715–722

    Article  PubMed  CAS  Google Scholar 

  • Dylis NV (1961) Larch of East Siberia and Far East. USSR Academy of Sciences, Moscow (in Russian)

    Google Scholar 

  • Farjon A (2001) World checklist and bibliography of Conifers. Royal Botanic Gardens, Kew

    Google Scholar 

  • Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7(7):1869–1885

    Article  PubMed  CAS  Google Scholar 

  • Gerlach WL, Dyer TA (1980) Sequence organization of the repeated units in the nucleus of wheat which contains 5S-rRNA genes. Nucleic Acids Res 8(21):4851–4865

    Article  PubMed  CAS  Google Scholar 

  • Hizume M (1988) Karyomorphological studies in the family Pinaceae. Mem Fac Educ Ehime University. Ser III. Natural Sci 8(2):1–108

    Google Scholar 

  • Hizume M, Tanaka A (1990) Fluorescent chromosome bandings in two American larches, Larix occidentalis and L. laricina. Kromosomo II 58:1979–1987

    Google Scholar 

  • Hizume M, Tominaga K, Kondo K, Gu Z, Yue Z (1993) Fluorescent chromosome banding in six taxa of Eurasian Larix, Pinaceae. Kromosomo II 69:2342–2354

    Google Scholar 

  • Hizume M, Kuzukawa Y, Konda K, Yang Q, Hong D, Tanaka R (1995) Localization of rDNAs and fluorescent bandings in chromosomes of Larix potaninii var. macrocarpa collected in Sichuan, China. Kromosomo II 78:2689–2694

    Google Scholar 

  • Hizume M, Kondo K, Zhang S, Hong D (1998) Fluorescence chromosome banding in a Chinese larch. Larix chinensis Beissn. Chromosome Sci 2(2):95–98

    Google Scholar 

  • Hizume M, Shibata F, Maruyama Y, Kondo T (2001) Cloning of DNA sequences localized on proximal fluorescent chromosome bands by microdissection in Pinus densiflora Sieb. & Zucc. Chromosoma 110:345–351

    Article  PubMed  CAS  Google Scholar 

  • Hizume M, Shibata F, Matsumoto A et al (2002) Tandem repeat DNA localizing on the proximal DAPI bands of chromosomes in Larix, Pinaceae. Genome 45:777–783

    Article  PubMed  CAS  Google Scholar 

  • Islam-Faridi NM, Nelson CD, Kubisiak TL et al. (2003) Loblolly pine Karyotype using FISH and DAPI positive banding. Proc. of the 27th Southern Forest Tree Improv. Conf. Stillwater, Oklahoma, pp. 184–188

  • Karvonen P, Karjalainen M, Savolainen O (1993) Ribosomal RNA genes in Scots pine (Pinus sylvestris L): chromosomal organization and structure. Genetica 88(1):59–68

    Article  CAS  Google Scholar 

  • Liu Z, Zhang D, Hong D, Wang X (2003) Chromosomal localization of 5S and 18S–5.8S-25S ribosomal DNA sites in five Asian pines using fluorescence in situ hybridization. Theor Appl Genet 106:198–204

    PubMed  CAS  Google Scholar 

  • Liu B, Zhang SG, Zhang Y, Lan TY, Qi LW, Song WQ (2006) Molecular cytogenetic analysis of four Larix species by bicolor fluorescence in situ hybridization and DAPI banding. Int J Plant Sci 167:367–372

    Article  Google Scholar 

  • Liu B, Qi L, Chen R, Song W (2007) Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii. Biol Res 40:23–28

    Article  PubMed  CAS  Google Scholar 

  • Lubaretz O, Fuchs J, Ahne R, Meister A, Schubert I (1996) Karyotyping of three Pinaceae species via fluorescent in situ hybridization and computer-aided chromosome analysis. Theor Appl Genet 92:411–416

    Article  Google Scholar 

  • Muratova EN (1991) Karyological studies in Larix sibirica (Pinaceae) from different parts of its area. Botanicheskyi zhurnal 76:1586–1595 (in Russian)

    Google Scholar 

  • Muratova EN (1994) Chromosome polymorphism in the natural populations of Gmelin larch, Larix gmelinii (Rupr.) Rupr. Cytologija i Genetika 28:14–22 (in Russian)

    Google Scholar 

  • Muratova EN, Sedelnikova TS, Pimenov AV, Karpjuk TV, Sizikh OA, Kvitko (Goryachkina) OV (2007) Karyological analysis of larch species from Siberia and the Far East of Russia. For Sci Tech 3:89–94

    Google Scholar 

  • Nkongolo KK, Klimaszewska K (1995) Cytological and molecular relationships between Larix decidua, L. leptolepis and Larix x eurolepis: identification of species-specific chromosoms and synchronization of mitotic cells. Theor Appl Genet 90:827–834

    Article  CAS  Google Scholar 

  • Ohri D, Khoshoo TN (1986) Genome size in gymnosperms. Plant Syst Evol 153:119–132

    Article  Google Scholar 

  • Patschke W (1913) Über die extratropische ostasiatischen Coniferen und ihre Bedeutung für die pflanzengeographische Gliederung von Ostasien. Bot Jahrb 48:651–655

    Google Scholar 

  • Polezhaeva MA, Lascoux M, Semerikov VL (2010) Cytoplasmic DNA variation and biogeography of Larix Mill. in Northeast Asia. Mol Ecol 19:1239–1252

    Article  PubMed  Google Scholar 

  • Ribeiro T, Barão A, Viegas W, Morais-Cecíli L (2008) Molecular cytogenetics of forest trees. Cytogenet Genome Res 120(3–4):220–227

    Article  PubMed  CAS  Google Scholar 

  • Semerikov VL, Polezhaeva MA (2007) Mitochondrial DNA variation pattern in larches of eastern Siberia and the Far East. Russ J Genet 43:646–652

    Article  CAS  Google Scholar 

  • Shibata F, Hizume M (2008) Comparative FISH karyotype analysis of 11 Picea species. Cytologia 73(2):203–211

    Article  Google Scholar 

  • Simak M (1966) Karyotype analysis of Larix griffithiana Carr. Hereditas 56:137–141

    Article  Google Scholar 

  • Sukachev VN (1924) To the history of larch development. In: The forest issues. Moscow-Leningrad, Novaya derevnya. pp. 12–44 (in Russian)

  • Wei X, Wang X (2003) Phylogenetic split of Larix: evidence from paternally inherited cpDNA trnT-trnF region. Plant Syst Evol 239:67–77

    Article  Google Scholar 

  • Zhang SG, Yang WH, Han SY, Han BT, Li MX, Qi LW (2010) Cytogenetic analysis of reciprocal hybrids and their parents between Larix leptolepis and Larix gmelinii: implications for identifying hybrids. Tree Genet Genomes 6(3):405–412

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Aleksander P. Isaev Ph.D. (Institute for Biological Problems of Cryolithozone SB RAS, Yakutsk), Alexey P. Barchenkov Ph.D. (V.N. Sukachev Institute of Forest SB RAS, Krasnoyarsk), and Candagdorj Jamiyansuren Ph.D. (Institute of Botany, Mongolian Academy of Sciences, Ulaanbaatar) for supplying the seed samples. This work was supported by grant no. 76 from the Integration Program of the Siberian Branch of the Russian Academy of Sciences and the Russian Foundation for Basic Research (Projects No. 11-04-00063 and 10-04-90780).

Author information

Authors and Affiliations

  1. V. N. Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Academgorodok Street 50/28, Krasnoyarsk, 660036, Russia

    Olga V. Goryachkina & Elena N. Muratova

  2. V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, Moscow, 119991, Russia

    Ekaterina D. Badaeva & Alexandr V. Zelenin

Authors
  1. Olga V. Goryachkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ekaterina D. Badaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elena N. Muratova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alexandr V. Zelenin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Olga V. Goryachkina.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goryachkina, O.V., Badaeva, E.D., Muratova, E.N. et al. Molecular cytogenetic analysis of Siberian Larix species by fluorescence in situ hybridization. Plant Syst Evol 299, 471–479 (2013). https://doi.org/10.1007/s00606-012-0737-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-012-0737-y

Keywords

Search

Navigation