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Banding and FISH in three species of Vernonia, subsection Macrocephalae (Asteraceae, Vernonieae)

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Abstract

To complement our knowledge about the karyotypes of the genus Vernonia Schreb., different techniques of chromosome banding, including AgNOR, triple staining with fluorochromes CMA/DA/DAPI (CDD), and fluorescence in situ hybridization (FISH) for the 45S rDNA probe, were applied to three species of subsection Macrocephalae. Vernonia bardanoides was collected from an area of cerrado (savanna) vegetation in Itirapina, São Paulo State, Brazil, and V. linearifolia and V. tomentella were collected from areas of rocky, open altitudinal vegetation in Joaquim Felicio and Diamantina, respectively, in Minas Gerais State. All species showed two terminal CMA+ and NOR bands. FISH indicated two terminal 45S rDNA sites in V. linearifolia and V. tomentella, and six in V. bardanoides.

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Acknowledgments

V.M.O was supported by a scholarship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and E.R.F.M. by a research fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). This work was funded by FAPESP (grant no. 04/13165-9).

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  1. Departamento de Botânica, Instituto de Biologia, CP6109, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil

    V. M. de Oliveira, J. Semir & E. R. Forni Martins

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  1. V. M. de Oliveira
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  2. J. Semir
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  3. E. R. Forni Martins
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Correspondence to E. R. Forni Martins.

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de Oliveira, V.M., Semir, J. & Forni Martins, E.R. Banding and FISH in three species of Vernonia, subsection Macrocephalae (Asteraceae, Vernonieae). Plant Syst Evol 298, 969–974 (2012). https://doi.org/10.1007/s00606-012-0606-8

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