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Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles for enrichment of phosphopeptides prior to their determination by MALDI-TOF mass spectrometry

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Abstract

Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were synthesized from resol, ZrO(NO3)2, ferric acetylacetonate, and triblock copolymer F127. The method involves a multi-component co-assembly strategy associated with direct carbonization. The resulting carbon material is shown to be useful as a metal oxide affinity chromatography (MOAC) material for enrichment of phosphopeptides owing to its large mesoporous (4.8 nm) surface area (442 m2 g−1), large pore volume (0.37 cm3 g−1) and excellent hydrophilicity. The metallic iron and ferric oxide particles modified on the mesoporous carbon exert a magnetic force and, in combination with the metallic zirconia, is a viable MOAC material for enrichment of low-abundance phosphopeptides. Because of metal chelation between metallic nanoparticles and the phosphate groups of phosphopeptides, the zirconia/magnetic mesoporous carbon displays high selectivity even at a phosphopeptide/nonphosphopeptide molar ratio of 1:500. As little as 1.5 fmol of phosphopeptides become detectable. The MOAC was successfully applied to the identification by MALDI-TOF MS of phosphopeptides in human serum and nonfat milk.

Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were prepared and successfully applied to the enrichment of low-abundance phosphopeptides from human serum and nonfat milk by MALDI-TOF mass spectrometry.

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Acknowledgements

This work was financially supported by the State Key Program of National Natural Science of China (21235005), the National Key Scientific Instrument and Equipment Development Project (2012YQ120044), the National Natural Science Foundation of China (21475044), the model Project of the Research and Application of the Common Key Technology about Chemical Reagent of Basis Scientific Research (2015BAK44B00) and foundation of Shanghai Research Institute of Criminal Science and Technology.

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Authors and Affiliations

  1. Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Lei Zhang, Yangyang Gan, Haofan Sun, Bohao Yu, Weibing Zhang & Lingyi Zhang

  2. China National Quality Supervision and Testing Center for Cu-Pb-Zn and Products, 1991 CuiHu Five Road, Tongling, Anhui, 244000, China

    Xiaofeng Jin

  3. Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Research Institute of Criminal Science and Technology, Shanghai Public Security Bureau, 803 ZhongShan North One Road, Shanghai, 200042, China

    Runsheng Zhang

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  1. Lei Zhang
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  2. Yangyang Gan
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  3. Haofan Sun
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  4. Bohao Yu
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  5. Xiaofeng Jin
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  6. Runsheng Zhang
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  7. Weibing Zhang
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  8. Lingyi Zhang
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Correspondence to Weibing Zhang or Lingyi Zhang.

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Zhang, L., Gan, Y., Sun, H. et al. Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles for enrichment of phosphopeptides prior to their determination by MALDI-TOF mass spectrometry. Microchim Acta 184, 547–555 (2017). https://doi.org/10.1007/s00604-016-2046-6

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