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One-step solvothermal preparation of silver-ZnO hybrid nanorods for use in enzymatic and direct electron-transfer based biosensing of glucose

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Abstract

This article describes an electrochemical glucose biosensor that is based on the immobilization of the enzyme glucose oxidase (GOx) on a glassy carbon electrode that was modified with silver-ZnO hybrid nanorods (HNRs). The HNRs containing different fractions of silver were synthesized by a one-step solvothermal method. Transmission electron microscopy, energy dispersive spectroscopy and X-ray diffraction confirmed the formation of these HNRs. FTIR spectroscopy and cyclic voltammetry demonstrated that the enzyme on the HNRs retained its native structure and bioactivity. The HNRs containing 11.26 wt% silver produced the best electrochemical response and direct electron transfer between enzyme and electrode surface. The sensor responds to glucose with two linear ranges (from 0.01 to 0.1 mM and from 0.1 to 1.5 mM). The sensitivity is 18.7 mA∙ M−1 ∙ cm−2, and the detection limit is 5 μM (at an SNR of 3). The biosensor is selective, acceptably stable, and well reproducible. It was successfully applied to the quantitation of glucose in human serum, and results were within −8.1 % and +6.5 % of data obtained with a reference method.

An electrochemical glucose biosensor was fabricated by immobilizing glucose oxidase (GOx) on silver-ZnO hybrid nanorods (Ag-ZnO HNRs), which were synthesized by a simple and low-cost one-step solvothermal route from Zn(NO3)2, AgNO3, ethanol and NaOH.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (21575125, 21475116, and 21275124), The Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD), University Natural Science Foundation of Jiangsu Province (13KJB150039) and the Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1410). We thank also The Testing Center of Yangzhou University for all the characterizations.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Environmental Material and Environmental Engineering, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, People’s Republic of China

    Juan Li, Mimi Lu, Zining Tan, Youbao Xu, Yongcai Zhang, Xiaoya Hu & Zhanjun Yang

Authors
  1. Juan Li
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  2. Mimi Lu
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  3. Zining Tan
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  4. Youbao Xu
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  5. Yongcai Zhang
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  6. Xiaoya Hu
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  7. Zhanjun Yang
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Corresponding authors

Correspondence to Xiaoya Hu or Zhanjun Yang.

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The author(s) declare that they have no competing interests

Additional information

Zhanjun Yang is the first corresponding author.

Highlights

• Silver-ZnO hybrid nanorods (Silver-ZnO HNRs) were synthesized by a facile method.

• Novel electrochemical glucose biosensor is presented based on silver-ZnO HNRs.

• The glucose biosensor showed excellent analytical performance.

• These silver-ZnO HNRs provide a promising matrix for biosensing applications.

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Li, J., Lu, M., Tan, Z. et al. One-step solvothermal preparation of silver-ZnO hybrid nanorods for use in enzymatic and direct electron-transfer based biosensing of glucose. Microchim Acta 183, 1705–1712 (2016). https://doi.org/10.1007/s00604-016-1800-0

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  • DOI: https://doi.org/10.1007/s00604-016-1800-0

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