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Glucose biosensor based on a platinum electrode modified with rhodium nanoparticles and with glucose oxidase immobilized on gold nanoparticles

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Abstract

We have developed an enzymatic glucose biosensor that is based on a flat platinum electrode which was covered with electrophoretically deposited rhodium (Rh) nanoparticles and then sintered to form a large surface area. The biosensor was obtained by depositing glucose oxidase (GOx), Nafion, and gold nanoparticles (AuNPs) on the Rh electrode. The electrical potential and the fractions of Nafion and GOx were optimized. The resulting biosensor has a very high sensitivity (68.1 μA mM−1 cm−2) and good linearity in the range from 0.05 to 15 mM (r = 0.989). The limit of detection is as low as 0.03 mM (at an SNR of 3). The glucose biosensor also is quite selective and is not interfered by electroactive substances including ascorbic acid, uric acid and acetaminophen. The lifespan is up to 90 days. It was applied to the determination of glucose in blood serum, and the results compare very well with those obtained with a clinical analyzer.

An enzymatic glucose biosensor was prepared based on rhodium nanoparticle modified Pt electrode and glucose oxidase immobilized in gold nanoparticles and Nafion composite film. The electrode showed a good response to glucose. The sensor was applied to the determination of glucose in blood serum.

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Acknowledgments

The research was supported by National Natural Science Foundation of China (Grant No. 30600143, 81171416), National Key Technology R&D Program of China (No. 2012BAI16B02), Zhejiang Provincial Natural Science Foundation of China (Grant No. Y13H180019).

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Authors and Affiliations

  1. Department of Biosystems Engineering, Zhejiang University, Hangzhou, 310058, China

    Xishan Guo & Jinming Jian

  2. Biosensor National Special Laboratory, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, 310027, China

    Bo Liang & Xuesong Ye

  3. Linyi University, Linyi, 276005, Shandong province, China

    Yelei Zhang

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  1. Xishan Guo
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  2. Bo Liang
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Correspondence to Yelei Zhang or Xuesong Ye.

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Guo, X., Liang, B., Jian, J. et al. Glucose biosensor based on a platinum electrode modified with rhodium nanoparticles and with glucose oxidase immobilized on gold nanoparticles. Microchim Acta 181, 519–525 (2014). https://doi.org/10.1007/s00604-013-1143-z

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  • DOI: https://doi.org/10.1007/s00604-013-1143-z

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