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Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats

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Abstract

Lead acetate (LA) is a toxic compound that has detrimental effects on the male reproductive system, such as decreased testicular size and function, low androgen hormone concentration, and altered testicular histology. While Nigella sativa (NS) has been shown to possess a handful of therapeutic benefits, few studies have shown its effect on damage caused by lead acetate toxicity in the male reproductive system. In this study, 75 Sprague-Dawley rats were divided into 3 groups of 25 rats, which were further subdivided into 5 subgroups each. Group 1 (negative control) was given distilled water, group 2 (positive control (PC)) administered 10 mg/kg of lead acetate (LA) orally/daily, while groups 3 (T1), 4 (T2), and 5 (T3) were given LA 10 mg/kg and graded concentrations (100, 150, and 200 mg/kg) each of NS. One group each, comprising of 25 rats, was euthanized at days 30, 60, and 90 for collection of blood plasma, epididymis, and organ tissues fixed in 10 % buffered formalin at each time interval. The right caudal epididymis was homogenized and used for the determination of spermiogram. Plasma was used for the determination of testosterone (TS), follicle stimulating hormone (FSH), and luteinizing hormone (LH) and estradiol (EST) using radioimmunoassay kits. There was reduced number of spermatogenic cells and epididymal sperm reserves in the PC group in comparison to the treatment groups. The level of TS was lower (p < 0.05) in the PC group at 90 days, while FSH was lower (p < 0.05) in T3 at 30 days and LH was higher (p < 0.05) in T1 at 90 days. The concentration of EST was lower (p < 0.05) in the PC, T1, and T2 at all time points, while the T3 group had the higher EST concentration that was similar to the control group. There was a decreased level of superoxide dismutase (SOD) and total glutathione (GSH) in the PC group and an increased GSH level in the T3 group. Sperm concentration, viability, and motility were adversely affected by LA, while concurrent treatment with NS significantly (p < 0.05) improved these parameters. This study showed the detrimental effects of LA on spermatogenesis, TS levels, and antioxidant defenses; however, these adverse effects were alleviated by oral NS administration.

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References

  • Abba Y, HassimH HH, Noordin MM (2015) Antioxidant vitamins, oxidant injuries and diseases. PJSRR 1:58–66

    Google Scholar 

  • Abbasnezhad A, Hayatdavoudi P, Niazmand S, Mahmoudabady M (2015) The effects of hydroalcoholic extract of Nigella sativa seed on oxidative stress in hippocampus of STZ-induced diabetic rats. Avicenna J Phytomed, 1–7

  • Ahamed M, Siddiqui M (2007) Low level lead exposure and oxidative stress: current opinions. CCA 383:57–64

    CAS  Google Scholar 

  • Ali B, Blunden G (2003) Pharmacological and toxicological properties of Nigella sativa. Phytother Res 17:299–305

    Article  CAS  PubMed  Google Scholar 

  • Allouche L, Hamadouche M, Touabti A (2009) Chronic effects of low lead levels on sperm quality, gonadotropins and testosterone in albino rats. Exp Toxicol Pathol 61:503–510

    Article  CAS  PubMed  Google Scholar 

  • Allouche L, Hamadouche M, Touabti A, Khennouf S (2011) Effect of long-term exposure to low or moderate lead concentrations on growth, lipid profile and liver function in albino rats. Adv Biol Res 5:339–347

    CAS  Google Scholar 

  • Al-Zahrani S, Mohany M, Kandeal S, Badr G (2012) Thymoquinone and vitamin E supplementation improve the reproductive characteristics of heat stressed male mice. J Med Plant Res 6:493–499

    CAS  Google Scholar 

  • Awadalla EA (2012) Ameliorative effect of the crude oil of the Nigella sativa on oxidative stress induced in rat testes by cisplatin treatment. Biomedicine and Preventive Nutrition 2:265–268

    Article  Google Scholar 

  • Bashandy AS (2007) Effect of fixed oil of Nigella sativa on male fertility in normal and hyperlipidemic rats. Int J Pharmacol 3:27–33

    Article  CAS  Google Scholar 

  • Bharali M (2013) Effect of acute lead acetate exposure on liver of mice. Journal of Global Biosciences 2:121–125

    Google Scholar 

  • Dalia M (2010) Effect of using pectin on lead toxicity. J Am Sci 6:541–554

    Google Scholar 

  • Dorostghoal M, Dezfoolian A, Sorooshnia F (2011) Effects of maternal lead acetate exposure during lactation on postnatal development of testis in offspring Wistar rats. Iran J Basic Med Sci 14:122–131

    Google Scholar 

  • Elgawish RAR, Abdelrazek HM (2014) Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats. Toxicology Reports 1:795–801

    Article  CAS  Google Scholar 

  • Elkhateeb A, El Khishin I, Megahed O, Mazen F (2015) Effect of Nigella sativa Linn oil on tramadol-induced hepato-and nephrotoxicity in adult male albino rats. Toxicology Reports 2:512–519

    Article  CAS  Google Scholar 

  • El-Tohamy M, El-Nattat W (2010) Effect of antioxidant on lead-induced oxidative damage and reproductive dysfunction in male rabbits. J Am Sci 6:613–622

    Google Scholar 

  • Gali-Muhtasib H, El-Najjar N, Schneider-Stock R (2006) The medicinal potential of black seed (Nigella sativa) and its components. Adv Phytomed 2:133–153

    Article  CAS  Google Scholar 

  • Hala M (2011) Protective effect of Nigella sativa, linseed and celery oils against testicular toxicity induced by sodium valproate in male rats. J Am Sci 7

  • Ibrahim NM, Eweis EA, El-Beltagi HS, Abdel-Mobdy YE (2012) Effect of lead acetate toxicity on experimental male albino rat. Asian Pac J Trop Biomed 2:41–46

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jahromy MH, Jalili M, Mohajer AJ, Poor FK, Dara SM (2014) Effects of Nigella sativa seed extract on perphenzine-induced muscle rigidity in male mice. World J Neurosci 2014

  • Jesse FFA, Abba Y, Tijjani A, Sadiq MA, Konto M, Adamu L, Wahid AH, MohdAzmi ML, Eric LTC, Ab Rahman MF (2016) Gonado-hypophyseal lesions and reproductive hormonal changes in Brucella melitensis-infected mice and its lipopolysaccharides (LPSs). Comp Clin Path 25:31–36

    Article  CAS  Google Scholar 

  • Juma FT, Hayfaa M (2011) A: the effects of Nigella sativa oil administration on some physiological and histological values of reproductive aspects of rats. Iraqi J Vet Med 35:52–60

    Google Scholar 

  • Krishnan N, Muthukrishnan S (2012) Effect of Nigella sativa seed extract on carbon tetrachloride-induced hepatotoxicity in rats. Acute Med 2:107–113

    Article  Google Scholar 

  • Mahdavi R, Heshmati J, Namazi N (2015) Effects of black seeds (Nigella sativa) on male infertility: a systematic review. J Herb Med 5:133–139

    Article  Google Scholar 

  • Makhlouf MMM, Eldien HMS, Zagloul DAM, Abu Dief EE, Abd ElHaliem NG (2008) The effect of the lead acetate on testicular structure and protective effect of vitamin E in adult albino rat. Egypt J Histol 31:406–418

  • Mann T, Lutwak-Mann C (2012) Male reproductive function and semen: themes and trends in physiology, biochemistry and investigative andrology. Springer Science & Business Media

  • Meyer O, Blom L, Sondergaard D (1982) The influence of minerals and protein on the nephrocalinosis potential for rats of semi synthesis diets. Lab Anim 16(3):271–273

    Article  CAS  PubMed  Google Scholar 

  • Murugesan M, Ragunath M, Prabu T, Nadanasabapathi S, Sakthivel M, Manju V (2012a) Protective role of black cumin (Nigella sativa) on isoproterenol induced myocardial infarction in rats. Int J Pharmacol and Clin Sci 1:45–53

    Google Scholar 

  • Murugesan M, Ragunath M, Prabu T, Nadanasabapathi S, Sakthivel M, Manju V (2012b) Protective role of black cumin (Nigella sativa) on isoproterenol induced myocardial infarction in rats. Int J Pharmacol Clin Sci 1:45–53

    Google Scholar 

  • Mustafa H N, Hussein AM (2015) Does allicin combined with vitamin B-complex have superior potentials than α-tocopherol alone in ameliorating lead acetate-induced Purkinje cell alterations in rats? An immunohistochemical and ultrastructural study. Folia Morphol

  • Papanikolaou NC, Hatzidaki EG, Belivanis S, Tzanakakis GN, Tsatsakis AM (2005) Lead toxicity update. A brief review. Med Sci Monit 11:RA329–RA336

    CAS  PubMed  Google Scholar 

  • Paul F (2009) Laboratory animal anesthesia. Academic press is an imprint of Elsevier. Newcastle University, pp. 208

  • Salem ML (2005) Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol 5:1749–1770

    Article  CAS  PubMed  Google Scholar 

  • Sharma R, Garu U (2011) Effects of lead toxicity on developing testes in Swiss mice. Univ J Environ Res Technol 1:390–398

    CAS  Google Scholar 

  • Weisbroth SH, Flatt RE Kraus AL (Eds). (2013) The biology of the laboratory rabbit. Academic Press

  • Yokoi K, Uthus EO, Nielsen FH (2003) Nickel deficiency diminishes sperm quantity and movement in rats. Biol Trace Elem Res 93(1–3):141–153

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We want to acknowledge the Ministry of Higher Education (MOHE), Malaysia, through its Fundamental Research Grant scheme for the financial support of this research.

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All authors contributed equally to this work.

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Authors and Affiliations

  1. Department of Community Health, College of Health and Medical Techniques, Al-Furat Al-Awsat Technical University, Babil, Iraq

    M. A. Assi

  2. Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    M. A. Assi, M. N. M. Hezmee & M. A. Rajion

  3. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Y. Abba & M. S. M. Yusof

  4. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    H. Wahid

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  1. M. A. Assi
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  6. M. S. M. Yusof
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Correspondence to M. N. M. Hezmee.

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Ethical statement

The animal experimental protocol used in this study was approved by the Institutional Animal Care and Use Committee (IACUC) with reference number: UPM/IACUC/AUP-R047/2015, in accordance with the standard guidelines on usage and care of laboratory animals.

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The authors declare that they have no conflict of interest.

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Assi, M.A., Hezmee, M.N.M., Abba, Y. et al. Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats. Comp Clin Pathol 26, 87–97 (2017). https://doi.org/10.1007/s00580-016-2349-3

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