Skip to main content

Advertisement

SpringerLink
Log in

Protective effects of olive leaf extract against reproductive toxicity of the lead acetate in rats

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

Lead acetate (PbAc) is one of the toxic metals in the environment which causes many effects on different organs of the body. And due to the importance of the olive tree, with its healthy and protective elements against many diseases, the leaf extract of this tree was chosen in our study. Therefore, the aim of this study was to investigate the role of olive leaf (Olea europea L.) extract (OLE) against PbAc-induced sperm toxicity, sex hormone changes, oxidative stress, and histopathological changes in rats. Twenty male Wistar rats were divided into four groups (group 1, as control; group 2, OLE; group 3, PbAc; group 4, PbAc+OLE). In the PbAc group, the body weight, testis and epididymis weights, sexual hormones, sperm characteristics, GR, GPx, GST, GSH, SOD, and CAT were significantly decreased, and the sperm abnormality and TBARS level were significant increase when compared with control and OLE groups. Also, numerous damages to testicular tissue were observed in the PbAc group when compared to the control group, while the treatment with OLE in the fourth group led to improvement of sex hormones, semen characteristics, oxidative stress, and testicular tissue damage caused by PbAc. It can be concluded that OLE has a protective and ameliorative effects against PbAc-induced oxidative stress, apoptosis and alterations in testicular tissue, and sperm quality in rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

References

  • Abd HH, Ahmed HA, Mutar TF (2020) Moringa oleifera leaves extract modulates toxicity, sperms alterations, oxidative stress, and testicular damage induced by tramadol in male rats. Toxicol Res (Camb)

  • Abdelhamid FM, Mahgoub HA, Ateya AI (2020) Ameliorative effect of curcumin against lead acetate–induced hemato-biochemical alterations, hepatotoxicity, and testicular oxidative damage in rats. Environ Sci Pollut Res 1–16

  • Abugomaa A, Elbadawy M (2020) Olive leaf extract modulates glycerol-induced kidney and liver damage in rats. Environ Sci Pollut Res 1–12

  • Al-Attar AM, Abu Zeid IM (2013) Effect of tea (Camellia sinensis) and olive (Olea europaea L.) leaves extracts on male mice exposed to diazinon. Biomed Res Int 2013:

  • Alirezaei M, Kheradmand A, Heydari R, Tanideh N, Neamati S, Rashidipour M (2012) Oleuropein protects against ethanol-induced oxidative stress and modulates sperm quality in the rat testis. Med J Nutrition Metab 5:205–211

    Google Scholar 

  • Almakhatreh M, Mutar TF, Tousson E (2020) Therapeutic role of rosemary extract against vepesid induced kidney injury. Proliferation and Apoptosis in Male Rats 3:16–25

    Google Scholar 

  • Almeer RS, Abdel Moneim AE (2018) Evaluation of the protective effect of olive leaf extract on cisplatin-induced testicular damage in rats. Oxid Med Cell Longev 2018

  • Al-Megrin WA, Alkhuriji AF, Yousef AOS et al (2020) Antagonistic efficacy of luteolin against lead acetate exposure-associated with hepatotoxicity is mediated via antioxidant, anti-inflammatory, and anti-apoptotic activities. Antioxidants 9:10

    Article  Google Scholar 

  • Al-Quraishy S, Othman MS, Dkhil MA, Moneim AEA (2017) Olive (Olea europaea) leaf methanolic extract prevents HCl/ethanol-induced gastritis in rats by attenuating inflammation and augmenting antioxidant enzyme activities. Biomed Pharmacother 91:338–349

    Article  CAS  Google Scholar 

  • Ansar S, AlGhosoon HT, Hamed S (2015) Evaluation of protective effect of rutin on lead acetate-induced testicular toxicity in Wistar rats. Toxin Rev 34:195–199

    Article  CAS  Google Scholar 

  • Ashkanani M, Farhadi B, Ghanbarzadeh E, Akbari H (2020) Study on the protective effect of hydroalcoholic olive leaf extract (oleuropein) on the testis and sperm parameters in adult male NMRI mice exposed to Mancozeb. Gene Reports 21:100870

    Article  Google Scholar 

  • Ashraf U, Kanu AS, Mo Z, Hussain S, Anjum SA, Khan I, Abbas RN, Tang X (2015) Lead toxicity in rice: effects, mechanisms, and mitigation strategies—a mini review. Environ Sci Pollut Res 22:18318–18332

    Article  CAS  Google Scholar 

  • Castejón ML, Montoya T, Alarcón-de-la-Lastra C, Sánchez-Hidalgo M (2020) Potential Protective role exerted by secoiridoids from Olea europaea L. in cancer, cardiovascular, neurodegenerative, aging-related, and immunoinflammatory diseases. Antioxidants 9:149

    Article  Google Scholar 

  • Cazarin CBB, da Silva JK, Colomeu TC, Batista ÂG, Meletti LMM, Paschoal JAR, Bogusz Junior S, Braga PAC, Reyes FGR, Augusto F, de Meirelles LR, Zollner RL, Maróstica Júnior MR (2015) Intake of Passiflora edulis leaf extract improves antioxidant and anti-inflammatory status in rats with 2, 4, 6-trinitrobenzenesulphonic acid induced colitis. J Funct Foods 17:575–586

    Article  CAS  Google Scholar 

  • Chiaino E, Micucci M, Cosconati S, Novellino E, Budriesi R, Chiarini A, Frosini M (2020) Olive leaves and hibiscus flowers extracts-based preparation protect brain from oxidative stress-induced injury. Antioxidants 9:806

    Article  CAS  Google Scholar 

  • De Cicco P, Maisto M, Tenore GC, Ianaro A (2020) Olive leaf extract, from Olea europaea L., reduces palmitate-induced inflammation via regulation of murine macrophages polarization. Nutrients 12:3663

    Article  Google Scholar 

  • Dkhil MA, Moneim AEA, Al-Quraishy S (2016) Indigofera oblongifolia ameliorates lead acetate-induced testicular oxidative damage and apoptosis in a rat model. Biol Trace Elem Res 173:354–361

    Article  CAS  Google Scholar 

  • Efentakis P, Iliodromitis EK, Mikros E, Papachristodoulou A, Dagres N, Skaltsounis AL, Andreadou I (2015) Effects of the olive tree leaf constituents on myocardial oxidative damage and atherosclerosis. Planta Med 81:648–654

    Article  CAS  Google Scholar 

  • Eldaim A, Tousson E, El Sayed IET, Awd WM (2019) Ameliorative effects of Saussurea lappa root aqueous extract against Ethephon-induced reproductive toxicity in male rats. Environ Toxicol 34:150–159

    Article  Google Scholar 

  • Elgawish RAR, Abdelrazek HMA (2014) Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats. Toxicol Rep 1:795–801

    Article  CAS  Google Scholar 

  • Fouad AA, Foda NT, Diab IH, Badr el Dine FMM, Balah MIF (2020) Evaluation of possible molecular toxicity induced by occupational exposure to lead and concomitant effect of smoking. Environ Sci Pollut Res 27:411–423

    Article  CAS  Google Scholar 

  • Gholami R, Zahedi SM (2019) Reproductive behavior and water use efficiency of olive trees (Olea europaea L. cv Konservolia) under deficit irrigation and mulching. Erwerbs-Obstbau 61:331–336

    Article  Google Scholar 

  • Guo J, Wu W, Sheng M et al (2013) Amygdalin inhibits renal fibrosis in chronic kidney disease. Mol Med Rep 7:1453–1457

    Article  CAS  Google Scholar 

  • Hari Priya P, Reddy PS (2012) Effect of restraint stress on lead-induced male reproductive toxicity in rats. J Exp Zool Part A Ecol Genet Physiol 317:455–465

    Article  CAS  Google Scholar 

  • Ibrahim NM, Eweis EA, El-Beltagi HS, Abdel-Mobdy YE (2012) Effect of lead acetate toxicity on experimental male albino rat. Asian Pac J Trop Biomed 2:41–46

    Article  CAS  Google Scholar 

  • Ileriturk M, Benzer F, Aksu EH, et al (2020) Chrysin protects against testicular toxicity caused by lead acetate in rats with its antioxidant, anti-inflammatory, and antiapoptotic properties. J Food Biochem e13593

  • Irakli M, Chatzopoulou P, Ekateriniadou L (2018) Optimization of ultrasound-assisted extraction of phenolic compounds: oleuropein, phenolic acids, phenolic alcohols and flavonoids from olive leaves and evaluation of its antioxidant activities. Ind Crop Prod 124:382–388

    Article  CAS  Google Scholar 

  • Kandeil MA, Abd Eltwab MA, Safwat GM (2020) Antioxidant role of vitamin C in alleviating the reproductive toxicity of lead acetate in male rats. J Adv Vet Res 10:9–12

    Google Scholar 

  • Lee-Huang S, Zhang L, Huang PL et al (2003) Anti-HIV activity of olive leaf extract (OLE) and modulation of host cell gene expression by HIV-1 infection and OLE treatment. Biochem Biophys Res Commun 307:1029–1037

    Article  CAS  Google Scholar 

  • Leskovec J, Rezar V, Nemec Svete A et al (2019) Antioxidative effects of olive polyphenols compared to vitamin e in piglets fed a diet rich in n-3 pufa. Animals 9:161

    Article  Google Scholar 

  • Lockyer S, Yaqoob P, Spencer JPE, Rowland I (2012) Olive leaf phenolics and cardiovascular risk reduction: physiological effects and mechanisms of action. Nutr Aging 1:125–140

    Article  Google Scholar 

  • López-Miranda J, Pérez-Jiménez F, Ros E, de Caterina R, Badimón L, Covas MI, Escrich E, Ordovás JM, Soriguer F, Abiá R, Alarcón de la Lastra C, Battino M, Corella D, Chamorro-Quirós J, Delgado-Lista J, Giugliano D, Esposito K, Estruch R, Fernandez-Real JM, Gaforio JJ, la Vecchia C, Lairon D, López-Segura F, Mata P, Menéndez JA, Muriana FJ, Osada J, Panagiotakos DB, Paniagua JA, Pérez-Martinez P, Perona J, Peinado MA, Pineda-Priego M, Poulsen HE, Quiles JL, Ramírez-Tortosa MC, Ruano J, Serra-Majem L, Solá R, Solanas M, Solfrizzi V, de la Torre-Fornell R, Trichopoulou A, Uceda M, Villalba-Montoro JM, Villar-Ortiz JR, Visioli F, Yiannakouris N (2010) Olive oil and health: summary of the II international conference on olive oil and health consensus report, Jaén and Córdoba (Spain) 2008. Nutr Metab Cardiovasc Dis 20:284–294

    Article  Google Scholar 

  • Mao X, Xia B, Zheng M, Zhou Z (2019) Assessment of the anti-inflammatory, analgesic and sedative effects of oleuropein from Olea europaea L. Cell Mol Biol 65:52–55

    Article  Google Scholar 

  • Meyer PA, Brown MJ, Falk H (2008) Global approach to reducing lead exposure and poisoning. Mutat Res Rev Mutat Res 659:166–175

  • Mokhtari M, Zanboori M (2011) The effects of lead acetate on sexual behavior and the level of testosterone in adult male rats. Int J Fertil Steril 5:13

    Google Scholar 

  • Mutar TF, Salem SB, Tousson E et al (2019) Ameliorative effects of vitamin B17 on the kidney against Ehrlich ascites carcinoma induced renal toxicity in mice. Environ Toxicol 35:1–10. https://doi.org/10.1002/tox.22888

    Article  CAS  Google Scholar 

  • Olmez E, Vural K, Gok S, Ozturk Z, Kayalar H, Ayhan S, Var A (2015) Olive leaf extract improves the atherogenic lipid profile in rats Fed a high cholesterol diet. Phyther Res 29:1652–1657

    Article  CAS  Google Scholar 

  • Park J-H, Jung J-H, Yang J-Y, Kim H-S (2013) Olive leaf down-regulates the oxidative stress and immune dysregulation in streptozotocin-induced diabetic mice. Nutr Res 33:942–951

    Article  CAS  Google Scholar 

  • Patra RC, Swarup D, Dwivedi SK (2001) Antioxidant effects of α tocopherol, ascorbic acid and L-methionine on lead induced oxidative stress to the liver, kidney and brain in rats. Toxicology 162:81–88

    Article  CAS  Google Scholar 

  • Patra RC, Rautray AK, Swarup D (2011) Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int 2011

  • Ponce-Canchihuamán JC, Pérez-Méndez O, Hernández-Muñoz R, Torres-Durán PV, Juárez-Oropeza MA (2010) Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney. Lipids Health Dis 9:35

    Article  Google Scholar 

  • Rahman A, Al-Awadi AA, Khan KM (2018) Lead affects vitamin D metabolism in rats. Nutrients 10:264

    Article  Google Scholar 

  • Rerknimitr P, Kantikosum K, Chottawornsak N, Tangkijngamvong N, Kerr SJ, Prueksapanich P, Sithisarankul P, Kumtornrut C, Asawanonda P, Sutheparuk S, Panchaprateep R (2019) Chronic occupational exposure to lead leads to significant mucocutaneous changes in lead factory workers. J Eur Acad Dermatology Venereol 33:1993–2000

    Article  CAS  Google Scholar 

  • Rostamzadeh A, Amini-Khoei H, Korani MJM, Rahimi-Madiseh M (2020) Comparison effects of olive leaf extract and oleuropein compounds on male reproductive function in cyclophosphamide exposed mice. Heliyon 6:e03785

    Article  Google Scholar 

  • Sarbishegi M, Gorgich EAC, Khajavi O et al (2018) The neuroprotective effects of hydro-alcoholic extract of olive (Olea europaea L.) leaf on rotenone-induced Parkinson’s disease in rat. Metab Brain Dis 33:79–88

    Article  CAS  Google Scholar 

  • Shan G, Tang T, Zhang X (2009) The protective effect of ascorbic acid and thiamine supplementation against damage caused by lead in the testes of mice. J Huazhong Univ Sci Technol Medical Sci 29:68–72

    Article  CAS  Google Scholar 

  • Sofuoglu SC, Yayla B, Kavcar P, Ates D, Turgut C, Sofuoglu A (2013) Olive tree, Olea europaea L., leaves as a bioindicator of atmospheric PCB contamination. Environ Sci Pollut Res 20:6178–6183

    Article  CAS  Google Scholar 

  • Soliman GA, Saeedan AS, Abdel-Rahman RF, Ogaly HA, Abd-Elsalam RM, Abdel-Kader MS (2019) Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: molecular and biochemical study. Saudi Pharm J 27:326–340

    Article  Google Scholar 

  • Taamalli A, Feriani A, Lozano-Sanchez J, Ghazouani L, el Mufti A, Allagui MS, Segura-Carretero A, Mhamdi R, Arráez-Roman D (2020) Potential hepatoprotective activity of super critical carbon dioxide olive leaf extracts against CCl4-induced liver damage. Foods 9:804

    Article  CAS  Google Scholar 

  • Tappel AL, Zalkin H (1959) Inhibition of lipide peroxidation in mitochondria by vitamin E. Arch Biochem Biophys 80:333–336

    Article  CAS  Google Scholar 

  • Toscano CD, Guilarte TR (2005) Lead neurotoxicity: from exposure to molecular effects. Brain Res Rev 49:529–554

  • Tousson E, El-Moghazy M, Massoud A, Akel A (2012) Histopathological and immunohistochemical changes in the testes of rabbits after injection with the growth promoter boldenone. Reprod Sci 19:253–259

    Article  CAS  Google Scholar 

  • Tousson E, Bayomy MF, Ahmed AA (2018) Rosemary extract modulates fertility potential, DNA fragmentation, injury, KI67 and P53 alterations induced by etoposide in rat testes. Biomed Pharmacother 98:769–774

    Article  CAS  Google Scholar 

  • Tousson E, Hafez E, Abo Gazia MM, Salem SB, Mutar TF (2020) Hepatic ameliorative role of vitamin B17 against Ehrlich ascites carcinoma–induced liver toxicity. Environ Sci Pollut Res 27:9236–9246. https://doi.org/10.1007/s11356-019-06528-6

    Article  CAS  Google Scholar 

  • Udefa AL, Amama EA, Archibong EA et al (2020) Antioxidant, anti-inflammatory and anti-apoptotic effects of hydro-ethanolic extract of Cyperus esculentus L.(tigernut) on lead acetate-induced testicular dysfunction in Wistar rats. Biomed Pharmacother 129:110491

    Article  CAS  Google Scholar 

  • Varmaghany S, Rahimi S, Torshizi MK, et al (2013) Effect of olive leaves on ascites incidence, hematological parameters and growth performance in broilers reared under standard and cold temperature conditions. Anim Feed Sci Technol 185:60–69

  • Vezza T, Rodríguez-Nogales A, Algieri F et al (2019) The metabolic and vascular protective effects of olive (Olea europaea L.) leaf extract in diet-induced obesity in mice are related to the amelioration of gut microbiota dysbiosis and to its immunomodulatory properties. Pharmacol Res 150:104487

    Article  CAS  Google Scholar 

  • Wei J, Wang S, Pei D, Qu L, Li Y, Chen J, di D, Gao K (2018) Antibacterial activity of hydroxytyrosol acetate from olive leaves (Olea Europaea L.). Nat Prod Res 32:1967–1970

    Article  CAS  Google Scholar 

  • Wulandari L, Retnaningtyas Y, Lukman H (2016) Analysis of flavonoid in medicinal plant extract using infrared spectroscopy and chemometrics. J Anal Methods Chem 2016

  • Zhang H, Hao F, Liu R (2013a) Interactions of lead (II) acetate with the enzyme lysozyme: a spectroscopic investigation. J Lumin 142:144–149

    Article  CAS  Google Scholar 

  • Zhang Y, Li Q, Liu X, Zhu H, Song A, Jiao J (2013b) Antioxidant and micronutrient-rich milk formula reduces lead poisoning and related oxidative damage in lead-exposed mice. Food Chem Toxicol 57:201–208

    Article  CAS  Google Scholar 

  • Zhang H, Liu Y, Zhang R, Liu R, Chen Y (2014) Binding mode investigations on the interaction of lead (II) acetate with human chorionic gonadotropin. J Phys Chem B 118:9644–9650

    Article  CAS  Google Scholar 

  • Žugčić T, Abdelkebir R, Alcantara C, Collado MC, García-Pérez JV, Meléndez-Martínez AJ, Režek Jambrak A, Lorenzo JM, Barba FJ (2019) From extraction of valuable compounds to health promoting benefits of olive leaves through bioaccessibility, bioavailability and impact on gut microbiota. Trends Food Sci Technol 83:63–77

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Environmental Studies, Faculty of Education For Pure Sciences, Al-Anbar University, Ramadi, Iraq

    Harith Abdulrhman Ahmed & Huda Abdullah Ali

  2. Department of Medical Laboratory Techniques, Al-maarif University College, Al-Anbar, Ramadi, Iraq

    Thulfiqar Fawwaz Mutar

Authors
  1. Harith Abdulrhman Ahmed
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huda Abdullah Ali
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thulfiqar Fawwaz Mutar
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors, Harith A. Ahmed, Huda A. Ali, and Thulfiqar F. Mutar, contributed to the study conception, design, material preparation, data collection, analysis, and approved the final manuscript.

Corresponding author

Correspondence to Thulfiqar Fawwaz Mutar.

Ethics declarations

Ethics approval and consent to participate

All rats were handled by the standard guide for the care and use of laboratory animals. The approval of the Institutional Animal Care and Use Committee for the experiment design was performed at the College of Veterinary Medicine, University of Baghdad, Iraq.

Consent for publication

Not applicable

Conflict of interest

The authors declare no competing interests.

Additional information

Responsible Editor: Mohamed M. Abdel-Daim

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ahmed, H.A., Ali, H.A. & Mutar, T.F. Protective effects of olive leaf extract against reproductive toxicity of the lead acetate in rats. Environ Sci Pollut Res 28, 63102–63110 (2021). https://doi.org/10.1007/s11356-021-15240-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-021-15240-3

Keywords

Search

Navigation