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Biebersteinia multifida DC a potential savior against testicular torsion-induced reperfusion injury

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Abstract

The study aimed to investigate the potential therapeutic effect of the methanolic extract of Biebersteinia multifida DC on ischemia–reperfusion injury induced by testicular torsion/detorsion in rats. Forty adult male Sprague-Dawley rats were randomly divided into four groups. Sham operation group, torsion/detorsion group plus saline, and torsion/detorsion groups plus 75 and 150 mg/kg doses of DC extract (administered intraperitoneally 15 min before detorsion). Testicular ischemia was induced via keeping the left testis under 720° counterclockwise torsion for 2 h; afterwards, detorsion was performed. All rats were sacrificed 4 h after detorsion and bilateral testes were removed for histological examinations. The testes in sham operation group had normal structure. Contralateral testicular tissue had mild injury in torsion/detorsion (T/D) group, while it was approximately normal in DC-treated group. In the T/D group, most of the testes demonstrated severe lesions in ipsilateral testes. In contrast, ipsilateral twisted testicular tissue in DC-treated group showed mild to moderate injuries. Similar histopathological results were obtained in both utilized therapeutic doses of DC (75 and 150 mg/kg). B. multifida is a rich source of phenolic antioxidant components that can be potentially used as a free radical scavenger in testicular damages caused by reperfusion injury, although more detailed studies are warranted.

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Acknowledgments

This research was financially supported by grants of Shiraz University Research Council.

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Authors and Affiliations

  1. Department of Anatomic Sciences, Anatomy and Embryology Division, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Younes Kamali & Soghra Gholami

  2. Department of Clinical Sciences, Veterinary Surgery Division, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Alireza Raayat Jahromi

  3. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Fatemeh Namazi

  4. Veterinary Undergraduate Student, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Amiarsalan Khorsand

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  1. Younes Kamali
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  2. Soghra Gholami
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  3. Alireza Raayat Jahromi
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  4. Fatemeh Namazi
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  5. Amiarsalan Khorsand
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Correspondence to Soghra Gholami.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed

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The authors declare that they have no conflict of interest.

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Kamali, Y., Gholami, S., Raayat Jahromi, A. et al. Biebersteinia multifida DC a potential savior against testicular torsion-induced reperfusion injury. Comp Clin Pathol 25, 1001–1005 (2016). https://doi.org/10.1007/s00580-016-2296-z

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  • DOI: https://doi.org/10.1007/s00580-016-2296-z

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