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Expression of metallothionein protein relating to proliferative cell index in malignant feline mammary tumors using high throughput tissue microarray technique

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Abstract

The present study aimed to examine the relationship between proliferation markers by detecting Ki-67 and proliferative cell nuclear antigen (PCNA) indices, and metallothionein expression, in different histological groups and nuclear grades of malignant feline mammary tumors by using immunohistochemistry and tissue microarray technique. Seventy feline mammary carcinoma (FMC) biopsy specimens were collected to perform tissue microarray (TMA) slide. The immunohistochemical staining was performed on the TMA slides against pancytokeratin (CK19), PCNA, Ki-67 clone MIB, and metallothionein 1 (MT-1) antibodies. The PCNA index showed no significant differences among histological types and nuclear grades, whereas the Ki-67 index showed statistical difference among histological types and malignancy grades (p < 0.05). The pattern of cytoplasmic MT expression was observed in glandular carcinoma cells. The percentage ± SD of MT expression was 44.13 ± 30.24 in tubular adenocarcinomas, 39.29 ± 26.21 in papillary adenocarcinomas, 22.66 ± 17.81 in cribriform carcinomas, and 38.12 ± 30.33 in solid carcinomas. The percentage ± SD of MT expression was 32.68 ± 27.59 in grade I, 37.34 ± 26.37 in grade II, and 43.54 ± 32.97 in grade III. Our study showed that the expression of MT increased with nuclear grade rather than histological subtype. It is suggested that the role of MT in carcinogenesis and tumor progression could be linked to involvement of MT in processes of cell proliferation and differentiation of feline mammary carcinomas.

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Acknowledgments

This research has been supported by the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University (CU-57-001-HR). We would like to thank Prof. Banchob Sripa, Experimental Pathology Unit, Department of Pathology, Faculty of Medicine, and Tropical Disease Research Laboratory, Khon Kaen University for kindly providing tissue microarray equipment. We would like to thank Mr. Supradit Wangnaitham and Mr. Suwit Balthaisong for technical assistance.

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Authors and Affiliations

  1. Companion Animal Cancer Research Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand

    Anudep Rungsipipat

  2. Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Panchan Sitthicharoenchai, Phimonrat Marlow & Perapux Prutthithaworn

  3. Department of Veterinary Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand

    Sirikachon Tangkawattana

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  1. Anudep Rungsipipat
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  2. Panchan Sitthicharoenchai
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  4. Perapux Prutthithaworn
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  5. Sirikachon Tangkawattana
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Correspondence to Anudep Rungsipipat.

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Rungsipipat, A., Sitthicharoenchai, P., Marlow, P. et al. Expression of metallothionein protein relating to proliferative cell index in malignant feline mammary tumors using high throughput tissue microarray technique. Comp Clin Pathol 25, 449–457 (2016). https://doi.org/10.1007/s00580-015-2208-7

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  • DOI: https://doi.org/10.1007/s00580-015-2208-7

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