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Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26

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Abstract

Ganoderma, as a Chinese traditional medicine, has multiple bioactivities. However, industrial production was limited due to low yield during Ganoderma fermentation. In this work, sucrose was found to greatly enhance intracellular polysaccharide (IPS) content and specific extracellular polysaccharide (EPS) production rate. The mechanism was studied by analyzing the activities of enzymes related to polysaccharide biosynthesis. The results revealed that sucrose regulated the activities of phosphoglucomutase and phosphoglucose isomerase. When glucose and sucrose mixture was used as carbon source, biomass, polysaccharide and ganoderic acids (GAs) production was greatly enhanced. A sucrose fed-batch strategy was developed in 10-L bioreactor, and was scaled up to 300-L bioreactor. The biomass, EPS and IPS production was 25.5, 2.9 and 4.8 g/L, respectively, which was the highest biomass and IPS production in pilot scale. This study provides information for further understanding the regulation mechanism of Ganoderma polysaccharide biosynthesis. It demonstrates that sucrose fed-batch is a useful strategy for enhancing Ganoderma biomass, polysaccharide and GAs production.

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Acknowledgments

This work was supported by post-doctoral station of Hangzhou Wahaha Group Co., Ltd. Financial support from the Post-doctor Foundation of Zhejiang Province, and sponsored by K. C. Wong Magna Fund in Ningbo University, Ningbo University School Research Fund (XYL15017),  Ningbo Agricultural Science and Technology Program (2014C10057) and Ningbo Natural Science Foundation (2014A610201).

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Authors and Affiliations

  1. School of Marine Sciences, Key Laboratory of Applied Marine Biotechnology (Ministry of Education), Ningbo University, Ningbo, 315211, China

    Zhen-hua Wei & Lianliang Liu

  2. Zhejiang Provincial Key Laboratory of Food Biotechnology, Institute of Bioengineering, Hangzhou Wahaha Group Co., Ltd., Hangzhou, 310018, China

    Zhen-hua Wei, Xiao-feng Guo, Yan-jun Li, Bao-chao Hou, Qiu-ling Fan & Kai-xiang Wang

  3. Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, 310027, China

    Yingdi Luo

  4. State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Jian-Jiang Zhong

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  1. Zhen-hua Wei
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  2. Lianliang Liu
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Correspondence to Lianliang Liu.

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Wei, Zh., Liu, L., Guo, Xf. et al. Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26. Bioprocess Biosyst Eng 39, 37–44 (2016). https://doi.org/10.1007/s00449-015-1480-x

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  • DOI: https://doi.org/10.1007/s00449-015-1480-x

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