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Dynamic changes of bacterial community under bioremediation with Sphingobium sp. LY-6 in buprofezin-contaminated soil

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Abstract

Buprofezin is a commonly used chemical with satisfactory biological activity against sucking insect pests, but its disposal can cause serious environmental problems. To study the feasibility of remedying contamination by buprofezin, microcosm experiments were carried out to study the effects of various concentrations of buprofezin and Sphingobium sp. LY-6 on soil bacterial communities in soils collected from vegetable fields. In this experiment, the results showed that buprofezin was effectively degraded by Sphingobium sp. LY-6 in incubation soils. Comparing to non-incubated soils, the cumulative degradation ratio of buprofezin was significantly increased, up to the extent of 85 and 51 %, in the initial concentration of 10 and 100 mg kg−1. The abundance and community structure of the bacterial communities were analysed by real-time PCR (qPCR) and terminal-restriction fragment length polymorphism (T-RFLP). The findings suggest that buprofezin had a negative effect on soil bacterial community, and decreases in bacterial abundance were observed in the later part of the incubation period. The bacterial community structure and diversity shifted significantly at each sampling time. In conclusion, the buprofezin-degrading strain LY-6 played a major role in the bioremediation of the buprofezin-contaminated soil and influenced the dynamics and structure of the bacterial community, demonstrating the great potential of exogenous microorganisms for soil remediation.

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Abbreviations

T-RFLP:

Terminal-restriction fragment length polymorphism

qPCR:

Quantitative real-time PCR

PCA:

Principal component analysis

HPLC:

High-performance liquid chromatography

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Acknowledgments

This work was supported by grants from the Chinese National Natural Science Foundation (31100083), the Foundation for Young Talents in College of Anhui Province, Provincial Natural Science Foundation of Anhui (1508085MC49) and Scholar Backbone Supporting Plan of Huaibei Normal University.

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Authors and Affiliations

  1. Department of Bioengineering, College of Life Sciences, Huaibei Normal University, 235000, Huaibei, Anhui Province, People’s Republic of China

    Yuan Liu, Qianqian Hou, Wanru Liu, Yawen Meng & Guangli Wang

  2. Institute of Resource, Ecosystem and Environment of Agriculture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu Province, People’s Republic of China

    Yuan Liu

Authors
  1. Yuan Liu
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  2. Qianqian Hou
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  3. Wanru Liu
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  4. Yawen Meng
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  5. Guangli Wang
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Correspondence to Guangli Wang.

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Liu, Y., Hou, Q., Liu, W. et al. Dynamic changes of bacterial community under bioremediation with Sphingobium sp. LY-6 in buprofezin-contaminated soil. Bioprocess Biosyst Eng 38, 1485–1493 (2015). https://doi.org/10.1007/s00449-015-1391-x

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  • DOI: https://doi.org/10.1007/s00449-015-1391-x

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