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Effects of Endosulfan on the Populations of Cultivable Microorganisms and the Diversity of Bacterial Community Structure in Brunisolic Soil

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Abstract

Endosulfan, an organochlorine pesticide, has been applied ubiquitously worldwide. However, endosulfan has been identified as a type of persistent organic pollutants (POPs), and its ecotoxicity has drawn attentions from scientists. The present study was implemented to examine the effects of endosulfan on the diversity and structure of soil microorganism communities. A control treatment and three concentrations (0.1, 1.0, and 10.0 mg/kg) were set up in laboratory experiments and sampled on days 7, 14, 21, and 28. The results revealed that the populations of bacteria and actinomycetes decreased significantly after 1.0 and 10.0 mg/kg treatments and that the soil microbial biomass carbon (MBC) was increased by endosulfan compared with the control. Terminal restriction fragment length polymorphism (T-RFLP) results revealed that the soil bacterial diversity was decreased by endosulfan and that the soil microbial community structure became unstable after endosulfan application. Moreover, the results of a 16S rRNA clone library revealed that the phyla Proteobacteria, Actinobacteria, Bacteroidetes, Spirochaetes, and Firmicutes showed an obvious advantage and closely relative. In conclusion, the results of the present study indicated that 0.1–10.0 mg/kg endosulfan showed obvious influences on the diversity and structure of the soil microbial community.

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Acknowledgements

The present study was supported by grants from the National Key Research and Development Plan [No. 2016YFD0800202] and the National Natural Science Foundation of China [Nos. 21377075 and 41671320].

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Authors and Affiliations

  1. College of Resources and Environment, Shandong Agricultural University, 61 Daizong Road, Taian, 271018, People’s Republic of China

    Xi Sun, Lusheng Zhu, Jinhua Wang, Jun Wang, Zhongkun Du & Pengpeng Guo

  2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, 8 Anwaidayangfang, Chaoyang District, Beijing, 100012, People’s Republic of China

    Benying Su

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  1. Xi Sun
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Sun, X., Zhu, L., Wang, J. et al. Effects of Endosulfan on the Populations of Cultivable Microorganisms and the Diversity of Bacterial Community Structure in Brunisolic Soil. Water Air Soil Pollut 228, 169 (2017). https://doi.org/10.1007/s11270-017-3357-x

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