Abstract
Biohydrogen production through water–gas shift (WGS) reaction by a biocatalyst was conducted in batch fermentation. The isolated photosynthetic bacterium Rhodopseudomonas palustris PT was able to utilize carbon monoxide and simultaneously produce hydrogen. Light exposure was provided as an indispensable requirement for the first stage of bacterial growth, but throughout the hydrogen production stage, the energy requirement was met through the WGS reaction. At ambient pressure and temperature, the effect of various sodium acetate concentrations in presence of CO-rich syngas on cell growth, carbon monoxide consumption, and biohydrogen production was also investigated. Maximal efficiency of hydrogen production in response to carbon monoxide consumption was recorded at 86 % and the highest concentration of hydrogen at 33.5 mmol/l was achieved with sodium acetate concentration of 1.5 g/l. The obtained results proved that the local isolate; R. palustris PT, was able to utilize CO-rich syngas and generate biohydrogen via WGS reaction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Demirbas A (2007) Progress and recent trends in biofuels. Prog Energy Combust Sci 33:1
Speight J (2008) Synthetic fuels handbook: properties, process and performance. McGraw-Hill Handbooks, New York
Steynberg AP, Espinoza RL, Jager B, Vosloo AC (1999) High temperature Fischer–Tropsch synthesis in commercial practice. Appl Catal A 186:41
Ni M, Leung DYC, Leung MKH, Sumathy K (2006) An overview of hydrogen production from biomass. Fuel Process Technol 87:461
Maness PC, Weaver PF (2002) Hydrogen production from a carbon-monoxide oxidation pathway in Rubrivivax gelatinosus. Int J Hydrogen Energy 27:1407
Mérida W, Maness PC, Brown RC, Levin DB (2004) Enhanced hydrogen production from indirectly heated, gasified biomass, and removal of carbon gas emissions using a novel biological gas reformer. Int J Hydrogen Energy 29:283
Grady JL, Chen GJ (1998) Bioconversion of waste biomass to useful products. US Patent 5,821,111, 1998 Google Patents
Jung GY, Kim JR, Park JY, Park S (2002) Hydrogen production by a new chemoheterotrophic bacterium Citrobacter sp. Y19. Int J Hydrogen Energy 27:601
Mohammadi M, Najafpour GD, Younesi H, Lahijani P, Uzir MH, Mohamed AR (2011) Bioconversion of synthesis gas to second generation biofuels: a review. Renew Sustain Energy Rev 15:4255
Younesi H, Najafpour G, Ku Ismail KS, Mohamed AR, Kamaruddin AH (2008) Biohydrogen production in a continuous stirred tank bioreactor from synthesis gas by anaerobic photosynthetic bacterium: Rhodospirillum rubrum. Bioresour Technol 99:2612
Henstra AM, Sipma J, Rinzema A, Stams AJM (2007) Microbiology of synthesis gas fermentation for biofuel production. Curr Opin Biotechnol 18:200
Pfennig N (1969) Rhodospirillum tenue sp. n., a new species of the purple nonsulfur bacteria. J Bacteriol 99:619
Najafpour G, Younesi H (2007) Bioconversion of synthesis gas to hydrogen using a light-dependent photosynthetic bacterium, Rhodospirillum rubrum. World J Microbiol Biotechnol 23:275
Giraud E, Fardoux J, Fourrier N, Hannibal L, Genty B, Bouyer P, Dreyfus B, Verméglio A (2002) Bacteriophytochrome controls photosystem synthesis in anoxygenic bacteria. Nature 417:202
Nath K, Das D (2009) Effect of light intensity and initial pH during hydrogen production by an integrated dark and photofermentation process. Int J Hydrogen Energy 34:7497
Lengeler JW, Drews G, Schlegel HG (1999) Biology of the Prokaryotes: Georg Thieme Verlag
Bailey JE, Ollis DF (1986) Biochemical engineering fundamentals. McGraw-Hill, New York
Parolini DN, Carcano S (2010) A model for cell growth in batch bioreactors. http://mox.polimi.it/it/progetti/pubblicazioni/tesi/carcano.pdf
Koku H, Eroğlu İ, Gündüz U, Yücel M, Türker L (2003) Kinetics of biological hydrogen production by the photosynthetic bacterium Rhodobacter sphaeroides OU 001. Int J Hydrogen Energy 28:381
Najafpour G, Younesi H, Mohamed AR (2004) Effect of organic substrate on hydrogen production from synthesis gas using Rhodospirillum rubrum, in batch culture. Biochem Eng J 21:123
Ferry JG (1995) CO dehydrogenase. Annu Rev Microbiol 49:305
Jung GY, Jung HO, Kim JR, Ahn Y, Park S (1999) Isolation and characterization of Rhodopseudomonas palustris P4 which utilizes CO with the production of H2. Biotechnol Lett 21:525
Najafpour G, Younesi H (2003) Effect of culture’s initial pH on hydrogen production from synthesis gas using Rhodospirillum rubrum. Inzynieria Mineralna. J Polish Mineral Eng Soc IV (2/11):29
Najafpour G, Younesi H, Mohamed AR (2006) A survey on various carbon sources for biological hydrogen production via the water-gas reaction using a photosynthetic bacterium Rhodospirillum rubrum. Energy Sources Part A 28:1013
Lee HT, Park J, Park S (2002) Growth of Rhodopseudomonas palustris under phototrophic and non-phototrophic conditions and its CO-dependent H2 production. Biotechnol Lett 24:91
Jung YG, Kim RJ, Jung OH, Park JY, Park S (1999) A new chemoheterotrophic bacterium catalyzing water-gas shift reaction. Biotechnol Lett 21:869
Acknowledgments
The authors gratefully acknowledge Iran National Science Foundation for partial financial support (Grant No.: 88000833) of this study which was conducted in Noshirvani University of Technology.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Pakpour, F., Najafpour, G., Tabatabaei, M. et al. Biohydrogen production from CO-rich syngas via a locally isolated Rhodopseudomonas palustris PT. Bioprocess Biosyst Eng 37, 923–930 (2014). https://doi.org/10.1007/s00449-013-1064-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-013-1064-6