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Synthesis of porcine pCLCA2 protein during late differentiation of keratinocytes of epidermis and hair follicle inner root sheath

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Abstract

Despite the discovery of the widely expressed CLCA (chloride channel regulators, calcium-activated) proteins more than 15 years ago, their seemingly diverse functions are still poorly understood. With the recent generation of porcine animal models for cystic fibrosis (CF), members of the porcine CLCA family are becoming of interest as possible modulators of the disease in the pig. Here, we characterize pCLCA2, the porcine ortholog of the human hCLCA2 and the murine mCLCA5, which are the only CLCA members expressed in the skin. Immunohistochemical studies with a specific antibody against pCLCA2 have revealed a highly restricted pCLCA2 protein expression in the skin. The protein is strictly co-localized with filaggrin and trichohyalin in the granular layer of the epidermis and the inner root sheath of the hair follicles, respectively. No differences have been observed between the expression patterns of wild-type pigs and CF transmembrane conductance regulator -/- pigs. We speculate that pCLCA2 plays an as yet undefined role in the structural integrity of the skin or, possibly, in specialized functions of the epidermis, including barrier or defense mechanisms.

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Acknowledgements

We are grateful to Jana Enders and Ursula Kobalz for technical support, to Astrid Bethe, Institute of Microbiology and Epizootics, Berlin, for kind help with the confocal laser scanning microscopic analyses, and to Heinz Kutzner, Friedrichshafen for supportive ideas.

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Authors and Affiliations

  1. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Straße 15, 14163, Berlin, Germany

    Stephanie Plog, Lars Mundhenk & Achim D. Gruber

  2. German Cancer Research Center, Genetics of Skin Carcinogenesis, A110, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany

    Lutz Langbein

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  1. Stephanie Plog
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  2. Lars Mundhenk
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  3. Lutz Langbein
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  4. Achim D. Gruber
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Correspondence to Achim D. Gruber.

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This study was supported by the German Research Foundation (Deutsche Forschungsgemeinschaft MU 3015/1-1), the Mukoviszidose Institut - gemeinnützige Gesellschaft für Forschung und Therapieentwicklung, and in part by the Wilhelm Sander-Stiftung, Munich (2007.133 to L.L.).

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Plog, S., Mundhenk, L., Langbein, L. et al. Synthesis of porcine pCLCA2 protein during late differentiation of keratinocytes of epidermis and hair follicle inner root sheath. Cell Tissue Res 350, 445–453 (2012). https://doi.org/10.1007/s00441-012-1482-9

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