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Cluster II che genes of Pseudomonas syringae pv. tabaci 6605, orthologs of cluster I in Pseudomonas aeruginosa, are required for chemotaxis and virulence

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Abstract

Pseudomonas syringae pv. tabaci 6605 (Pta6605) is a causal agent of wildfire disease in host tobacco plants and is highly motile. Pta6605 has multiple clusters of chemotaxis genes including cheA, a gene encoding a histidine kinase, cheY, a gene encoding a response regulator, mcp, a gene for a methyl-accepting chemotaxis protein, as well as flagellar and pili biogenesis genes. However, only two major chemotaxis gene clusters, cluster I and cluster II, possess cheA and cheY. Deletion mutants of cheA or cheY were constructed to evaluate their possible role in Pta6605 chemotaxis and virulence. Motility tests and a chemotaxis assay to known attractant demonstrated that cheA2 and cheY2 mutants were unable to swarm and to perform chemotaxis, whereas cheA1 and cheY1 mutants retained chemotaxis ability almost equal to that of the wild-type (WT) strain. Although WT and cheY1 mutants of Pta6605 caused severe disease symptoms on host tobacco leaves, the cheA2 and cheY2 mutants did not, and symptom development with cheA1 depended on the inoculation method. These results indicate that chemotaxis genes located in cluster II are required for optimal chemotaxis and host plant infection by Pta6605 and that cluster I may partially contribute to these phenotypes.

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Acknowledgements

The first author was supported by the Japanese Government through a MONBUKAGAKUSHO (MEXT) Scholarship during her study at Okayama University. The Pseudomonas syringae pv. tabaci 6605 isolate was kindly provided by the Leaf Tobacco Research Laboratory of Japan Tobacco Inc. We thank Prof. J. Kato and Dr. S. Oku, Hiroshima University, for the technical advice on the chemotaxis test. This work was supported in part by Grants-in-Aid for Scientific Research (Nos. 26660035 and 19H02956) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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  1. Fumiko Taguchi

    Present address: Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

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  1. Graduate School of Environmental and Life Science, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, Japan

    Stephany Angelia Tumewu, Fumiko Taguchi, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda & Yuki Ichinose

  2. Faculty of Agriculture, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, Japan

    Yujiro Ogawa, Takumi Okamoto, Yuka Sugihara & Hajime Yamada

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  1. Stephany Angelia Tumewu
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Tumewu, S.A., Ogawa, Y., Okamoto, T. et al. Cluster II che genes of Pseudomonas syringae pv. tabaci 6605, orthologs of cluster I in Pseudomonas aeruginosa, are required for chemotaxis and virulence. Mol Genet Genomics 296, 299–312 (2021). https://doi.org/10.1007/s00438-020-01745-y

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