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Chromosomal evolutionary dynamics of four multigene families in Coreidae and Pentatomidae (Heteroptera) true bugs

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Abstract

Previous chromosome mapping of multigene families in Pentatomomorpha (Heteroptera) insects, which was restricted to the major rDNA, revealed remarkable conservation of number of clusters and chromosomal positions. Aiming to understand the chromosomal organization and evolutionary patterns of multigene families in karyotypes of Heteroptera, we performed a chromosomal mapping using four distinct multigene families in representatives of Coreidae (ten species) and Pentatomidae (five species). A single pair of the major rDNA cluster (18S rDNA probe) and a single pair of the minor rDNA cluster (5S rDNA probe), both terminally located were primarily observed, being, in most species, located in distinct chromosomes. However, some alternative patterns were also observed. In species in which the U2 snDNA and H4 gene clusters were mapped, they were mainly located in one autosomal pair each, wherein the H4 gene cluster was located in different positions. Our data suggest that the karyotype diversity reported in Coreidae is not reflected in the distribution diversity of multigene families. This contrasts with the data for Pentatomidae, with a conserved gross karyotype but a discrete diversity in the location of the clusters of multigene families, indicating genome dynamics for these markers. The findings are discussed to shed light on the possible causes for the conservation or variation observed and to assist in understanding the chromosomal evolutionary trends in the group.

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Acknowledgments

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Process Number 2014/11763-8), Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). We are also grateful to the anonymous reviewers for valuable comments. DCCM and JAMF were the recipients of a research productivity fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (Process Numbers 304758/2014-0, 311403/2012-3, respectively) and VBB was a recipient of PNPD scholarship.

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Authors and Affiliations

  1. Departamento de Biologia, Instituto de Biociências/IB, UNESP-Universidade Estadual Paulista, Rio Claro, 13506-900, Brazil

    Vanessa Bellini Bardella & Diogo Cavalcanti Cabral-de-Mello

  2. Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Belém, Brazil

    José Antônio Marin Fernandes

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  1. Vanessa Bellini Bardella
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  2. José Antônio Marin Fernandes
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  3. Diogo Cavalcanti Cabral-de-Mello
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Correspondence to Diogo Cavalcanti Cabral-de-Mello.

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Funding

This study was founded by Fundação de Amparo a Pesquisa do Estado de São Paulo-FAPESP (Process Number 2014/11763-8) and Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior-CAPES.

Conflict of interest

Vanessa Bellini Bardella declares that she has no conflict of interest. José Antônio Marin Fernandes declares that he has no conflict of interest. Diogo Cavalcanti Cabral-de-Mello declares that he has no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Communicated by Q. Xia.

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Bardella, V.B., Fernandes, J.A.M. & Cabral-de-Mello, D.C. Chromosomal evolutionary dynamics of four multigene families in Coreidae and Pentatomidae (Heteroptera) true bugs. Mol Genet Genomics 291, 1919–1925 (2016). https://doi.org/10.1007/s00438-016-1229-5

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  • DOI: https://doi.org/10.1007/s00438-016-1229-5

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