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Worm expulsion of Gymnophalloides seoi from C57BL/6 mice: role of metacercarial exosomes in upregulating TLR2 and MUC2 expression in intestinal tissues

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Abstract

Gymnophalloides seoi worms were rapidly expelled from C57BL/6 mice within days 3–6 post-infection probably due to operation of mucosal innate immunity. To understand better the mucosal immunity related to worm expulsion from the host, we isolated exosomes of G. seoi metacercariae and investigated their role in induction of mRNA and protein expression of several Toll-like receptors and mucin-related factors in vitro. G. seoi-secreted exosomes were collected using differential ultracentrifugation, and cellular internalization of the exosomes into HT-29 intestinal epithelial cells was visualized by confocal microscopy. The expression of TLR2 and MUC2 in HT-29 cells was up-regulated in stimulation with the exosomes. We suggest that G. seoi-secreted exosomes offer a new point of view in the mechanism of worm expulsion from the host through enhancement of TLR2 and MUC2 expression.

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Funding

This study was funded by project no. 2011-0024699 from National Research Foundation of Korea (NRFK).

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Authors and Affiliations

  1. Korea Association of Health Promotion, Institute of Parasitic Diseases, Seoul, 07649, South Korea

    Hyemi Song, Bong-Kwang Jung, Jaeeun Cho & Jong-Yil Chai

  2. Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul, 03080, South Korea

    Jong-Yil Chai

Authors
  1. Hyemi Song
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  2. Bong-Kwang Jung
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  3. Jaeeun Cho
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  4. Jong-Yil Chai
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Corresponding author

Correspondence to Jong-Yil Chai.

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All animal experiments were approved by the Committee on the Ethics of Animal Experiments at Seoul National University (permit no. SNU-121010-4-1).

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The authors declare that they have no conflict of interest.

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Section Editor: Xing-Quan Zhu

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Song, H., Jung, BK., Cho, J. et al. Worm expulsion of Gymnophalloides seoi from C57BL/6 mice: role of metacercarial exosomes in upregulating TLR2 and MUC2 expression in intestinal tissues. Parasitol Res 117, 3309–3314 (2018). https://doi.org/10.1007/s00436-018-6002-8

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  • DOI: https://doi.org/10.1007/s00436-018-6002-8

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